DataVXAdvanced SciencePaper
journal article·2019-11Open access

Gene Delivery to Nonhuman Primate Preimplantation Embryos Using Recombinant Adeno‐Associated Virus

Dan Wang, Yuyu Niu, Lingzhi Ren, Yu Kang, Phillip W. L. Tai, Chenyang Si, Craig A. Mendonca, Hong Ma, Guangping Gao, Weizhi Ji

Advanced Science· Volume 6· Issue 21
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Source abstract

Abstract

Abstract Delivery of genome editing tools to mammalian zygotes has revolutionized animal modeling. However, the mechanical delivery method to introduce genes and proteins to zygotes remains a challenge for some animal species that are important in biomedical research. Here, an approach to achieve gene delivery and genome editing in nonhuman primate embryos is presented by infecting zygotes with recombinant adeno‐associated viruses (rAAVs). Together with previous reports from the authors of this paper and others, this approach is potentially applicable to a broad range of mammals. In addition to genome editing and animal modeling, this rAAV‐based method can facilitate gene function studies in early‐stage embryos.
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Research context

OpenAlex topics: CRISPR and Genetic Engineering · Virus-based gene therapy research · Viral Infectious Diseases and Gene Expression in Insects

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Horae Gene Therapy Center University of Massachusetts Medical School Worcester MA 01605 USA

Department of Microbiology and Physiological Systems University of Massachusetts Medical School Worcester MA 01605 USA

Yunnan Key Laboratory of Primate Biomedicine Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 Yunnan China

Li Weibo Institute for Rare Diseases Research University of Massachusetts Medical School Worcester MA 01605 USA

Funding

University of Massachusetts Medical School · Ministry of Science and Technology of the People's Republic of China

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