DataVXAdvanced SciencePaper
journal article·2026-04Open access

Bioinspired Engineering of Streamlined Skeletal Interoception: Neural Bioprinted Piezoelectric Scaffolds for Neuro‐Vascularized Bone Regeneration

Yingze Su, Haomin Wang, Weixi Liu, Kangming Chen, Yiyang Min, Jinbo Zhu, Xueyang Li, Anning Su, Hao Yang, Lei Yang, Yun Ji, Yuxin Zhang, Jisi Zheng, Chi Yang, Chuanglong He, Tao Li, Shuo Chen, Tao Wu, Xiaodong Chen

Advanced Science· Volume 13· Issue 21
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Source abstract

Abstract

ABSTRACT The skeletal interoception system that orchestrates bone homeostasis and regeneration through a coordinated “afferent‐integration‐efferent” reflex arc has been overlooked in bone tissue engineering. The dorsal root ganglion (DRG) neurons constitute the hubs of this system. Moreover, beyond their canonical role within the interoceptive pathway, these neurons exert a direct effector role by secreting calcitonin gene‐related peptide (CGRP) to promote bone repair. However, current sensory nerve‐targeted strategies in bone tissue engineering remain static, bypassing the dynamic reflex arc and failing to establish an autonomous functional system. To address this, a streamlined interoception unit was engineered using neural bioprinting of DRG neurons within piezoelectric scaffolds. Upon sensing ultrasound (US) stimulation, the piezoelectric poly(l‐lactide) (PLLA) component mediates mechanoelectrical coupling, triggering a Ca 2 + influx‐induced effector response in the incorporated DRG neurons that enhances CGRP secretion and expression. The secreted CGRP subsequently promotes osteogenesis and angiogenesis in vitro and accelerates neuro‐vascularized bone regeneration in a rat femoral condyle defect model. This study established a bioinspired platform with a self‐contained “sensor‐effector” circuit, offering a novel engineered strategy for bone regeneration.
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Research context

Biomaterials

OpenAlex topics: 3D Printing in Biomedical Research · Planarian Biology and Electrostimulation · Bone Tissue Engineering Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Orthopaedics Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China

State Key Lab of Tropic Ocean Engineering Materials and Materials Evaluation Hainan University Haikou People's Republic of China

Department of Orthopaedics Huashan Hospital Fudan University Shanghai People's Republic of China

Department of Otorhinolaryngology Head and Neck Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China

Department of Orthopaedics the Second Hospital of Dalian Medical University Dalian People's Republic of China

Department of Biomedical Engineering University of Shanghai for Science and Technology Shanghai People's Republic of China

Department of Orthopaedics Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China

State Key Laboratory of Advanced Fiber Materials College of Biological Science and Medical Engineering Donghua University Shanghai People's Republic of China

Department of Pain Medicine Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China

Department of Oral Surgery Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai People's Republic of China

Shanghai University of Medicine & Health Sciences Shanghai People's Republic of China

Funding

National Key Research and Development Program of China · National Natural Science Foundation of China · Natural Science Foundation of Hunan Province · China Postdoctoral Science Foundation

References (52)

Show source-provided references
1. 10.1038/s41580‐020‐00279‐w10.1038/s41580‐020‐00279‐w ↗
2. 10.1007/s10856‐014‐5240‐210.1007/s10856‐014‐5240‐2 ↗
3. 10.1302/2046‐3758.73.BJR‐2017‐0270.R110.1302/2046‐3758.73.BJR‐2017‐0270.R1 ↗
4. 10.1186/1749‐799X‐8‐3310.1186/1749‐799X‐8‐33 ↗
5. 10.1016/j.addr.2025.11565610.1016/j.addr.2025.115656 ↗
6. 10.1016/j.biomaterials.2025.12393810.1016/j.biomaterials.2025.123938 ↗
7. 10.1002/adma.20251149710.1002/adma.202511497 ↗
8. 10.1002/adhm.20230092710.1002/adhm.202300927 ↗
9. 10.1038/s41413‐023‐00285‐610.1038/s41413‐023‐00285‐6 ↗
10. 10.1002/advs.20230504210.1002/advs.202305042 ↗
11. 10.1016/j.cmet.2022.09.02510.1016/j.cmet.2022.09.025 ↗
12. 10.1002/advs.20250103910.1002/advs.202501039 ↗
13. 10.1016/j.autneu.2013.05.00510.1016/j.autneu.2013.05.005 ↗
14. 10.1016/j.bone.2010.02.02210.1016/j.bone.2010.02.022 ↗
15. 10.1002/jbmr.382210.1002/jbmr.3822 ↗
16. 10.1038/s41467‐018‐08097‐710.1038/s41467‐018‐08097‐7 ↗
17. 10.1007/s11914‐020‐00624‐010.1007/s11914‐020‐00624‐0 ↗
18. 10.1038/nm.416210.1038/nm.4162 ↗
19. 10.1016/j.biomaterials.2021.12098410.1016/j.biomaterials.2021.120984 ↗
20. 10.1016/j.bjoms.2006.08.00810.1016/j.bjoms.2006.08.008 ↗
21. 10.1016/j.mtbio.2025.10175010.1016/j.mtbio.2025.101750 ↗
22. 10.1039/D5TB00113G10.1039/D5TB00113G ↗
23. 10.1126/sciadv.adg738010.1126/sciadv.adg7380 ↗
24. 10.1016/j.reth.2025.02.01610.1016/j.reth.2025.02.016 ↗
25. 10.1021/acsami.4c1716210.1021/acsami.4c17162 ↗
26. 10.1002/advs.20210300510.1002/advs.202103005 ↗
27. 10.1042/cs070038910.1042/cs0700389 ↗
28. 10.1016/j.ymthe.2025.04.00410.1016/j.ymthe.2025.04.004 ↗
29. 10.1016/j.colsurfb.2025.11454410.1016/j.colsurfb.2025.114544 ↗
30. 10.1021/acsami.3c0923710.1021/acsami.3c09237 ↗
31. 10.1002/adma.20240100910.1002/adma.202401009 ↗
32. 10.1002/adma.20240940010.1002/adma.202409400 ↗
33. Release of Substance P from Peripheral Sensory Nerve Terminals
34. 10.3748/wjg.15.15110.3748/wjg.15.151 ↗
35. 10.1002/adma.20250759010.1002/adma.202507590 ↗
36. 10.1063/5.027670410.1063/5.0276704 ↗
37. 10.3390/mi1208086510.3390/mi12080865 ↗
38. 10.1016/j.biomaterials.2025.12352210.1016/j.biomaterials.2025.123522 ↗
39. 10.1021/acsami.5c0255710.1021/acsami.5c02557 ↗
40. 10.1038/s41467‐023‐41594‐y10.1038/s41467‐023‐41594‐y ↗
41. 10.1038/s41467‐018‐03759‐y10.1038/s41467‐018‐03759‐y ↗
42. 10.1186/s10194‐019‐1037‐510.1186/s10194‐019‐1037‐5 ↗
43. 10.1002/adfm.20210987110.1002/adfm.202109871 ↗
44. 10.1111/j.1749‐6632.1992.tb22767.x10.1111/j.1749‐6632.1992.tb22767.x ↗
45. 10.3390/cells808078410.3390/cells8080784 ↗
46. 10.1002/advs.20240024210.1002/advs.202400242 ↗
47. 10.1038/nprot.2008.22110.1038/nprot.2008.221 ↗
48. 10.1016/j.jot.2014.07.00510.1016/j.jot.2014.07.005 ↗
49. 10.1126/science.275.5302.96410.1126/science.275.5302.964 ↗
50. Simultaneous Isolation of Mesenchymal Stem Cells and Endothelial Progenitor Cells Derived from Murine Bone Marrow
51. 10.1016/j.bioactmat.2021.07.01610.1016/j.bioactmat.2021.07.016 ↗
52. 10.1016/j.biomaterials.2012.06.02110.1016/j.biomaterials.2012.06.021 ↗