Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens

Abstract Molecular diagnosis limitations, including complex treatment processes, low cost‐effectiveness, and operator‐dependent low reproducibility, interrupt the timely prevention of disease spread and the development of medical devices for home and outdoor uses. A newly fabricated gold nanopillar array‐based film is presented for superior photothermal energy conversion. Magnifying the metal film surface‐to‐volume ratio increases the photothermal energy conversion efficiency, resulting in a swift reduction in the gene amplification reaction time. Plasmonic energy‐based ultrafast gene amplification and facile confirmation methodology offer a rapid disease discrimination platform for high‐throughput multiplexed diagnosis. The superior performance of the gold nanopillar arrayed film is demonstrated by measuring the amount of pathogen (Vibrio cholerae) with a sensitivity of 10¹ cfu mL⁻¹ in 5.5 min. The newly engineered gold nanopillar arrayed film can be utilized to diagnose universal pathogens to achieve an increasingly successful complete cure.