DataVXAdvanced SciencePaper
journal article·2024-09Open access

Optogenetic Strategies for Optimizing the Performance of Phospholipids Biosensors

Yuanfa Yao, Xiayan Lou, Luhong Jin, Weiyun Sun, Jingfang Liu, Yunyue Chen, Sunying Cheng, Tengjiao Zhao, Shuwei Ke, Luhao Zhang, Yingke Xu, Lian He, Hanbing Li

Advanced Science· Volume 11· Issue 36
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Source abstract

Abstract

Abstract High‐performance biosensors play a crucial role in elucidating the intricate spatiotemporal regulatory roles and dynamics of membrane phospholipids. However, enhancing the sensitivity and imaging performance remains a significant challenge. Here, optogenetic‐based strategies are presented to optimize phospholipid biosensors. These strategies involves presequestering unbound biosensors in the cell nucleus and regulating their cytosolic levels with blue light to minimize background signal interference in phospholipid detection, particularly under conditions of high expression levels of biosensor. Furthermore, optically controlled phase separation and the SunTag system are employed to generate punctate probes for substrate detection, thereby amplifying biosensor signals and enhancing visualization of the detection process. These improved phospholipid biosensors hold great potential for enhancing the understanding of the spatiotemporal dynamics and regulatory roles of membrane lipids in live cells and the methodological insights in this study might be valuable for developing other high‐performance biosensors.
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Research context

OpenAlex topics: Photoreceptor and optogenetics research · Molecular Communication and Nanonetworks · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Pharmacology College of Pharmaceutical Science Zhejiang University of Technology Hangzhou 310014 China

School of Information Science and Technology Hangzhou Normal University Hangzhou Zhejiang 311121 China

Department of Biomedical Engineering Key Laboratory of Biomedical Engineering of Ministry of Education State Key Laboratory of Extreme Photonics and Instrumentation Zhejiang Provincial Key Laboratory of Cardio‐Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal Zhejiang University Hangzhou 310027 China

Department of Endocrinology Children's Hospital of Zhejiang University School of Medicine National Clinical Research Center for Children's Health Hangzhou Zhejiang 310051 China

Department of Pharmacology Joint Laboratory of Guangdong‐Hong Kong Universities for Vascular Homeostasis and Diseases School of Medicine Southern University of Science and Technology Shenzhen 518055 China

Funding

Natural Science Foundation of Zhejiang Province · Key Technologies Research and Development Program

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