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journal article·2024-12Open access

In Vivo Assessment of Deep Vascular Patterns in Murine Colitis Using Optoacoustic Mesoscopic Imaging

Adrian Buehler, Emma L. Brown, Emmanuel Nedoschill, Markus Eckstein, Petra Ludwig, Felix Wachter, Henriette Mandelbaum, Roman Raming, Mariam‐Eleni Oraiopoulou, Lars‐Philip Paulus, Ulrich Rother, Oliver Friedrich, Markus F. Neurath, Joachim Woelfle, Maximilian J. Waldner, Ferdinand Knieling, Sarah E. Bohndiek, Adrian P. Regensburger

Advanced Science· Volume 11· Issue 45
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Source abstract

Abstract

Abstract The analysis of vascular morphology and functionality enables the assessment of disease activity and therapeutic effects in various pathologies. Raster‐scanning optoacoustic mesoscopy (RSOM) is an imaging modality that enables the visualization of superficial vascular networks in vivo. In murine models of colitis, deep vascular networks in the colon wall can be visualized by transrectal absorber guide raster‐scanning optoacoustic mesoscopy (TAG‐RSOM). In order to accelerate the implementation of this technology in translational studies of inflammatory bowel disease, an image‐processing pipeline for TAG‐RSOM data has been developed. Using optoacoustic data from a murine model of chemically‐induced colitis, different image segmentation methods are compared for visualization and quantification of deep vascular patterns in terms of vascular network length and complexity, blood volume, and vessel diameter. The presented image‐processing pipeline for TAG‐RSOM enables label‐free in vivo assessment of changes in the vascular network in murine colitis with broad applications for inflammatory bowel disease research.
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Research context

OpenAlex topics: Photoacoustic and Ultrasonic Imaging · Thermography and Photoacoustic Techniques · Optical Imaging and Spectroscopy Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Pediatrics and Adolescent Medicine University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen Germany

Cancer Research UK Cambridge Institute University of Cambridge CB2 0RE Cambridge United Kingdom

Institute of Pathology Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen Germany

Department of Vascular Surgery University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91054 Erlangen Germany

Institute of Medical Biotechnology Department of Chemical and Biological Engineering Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91052 Erlangen Germany

Department of Medicine 1 University Hospital Erlangen Friedrich‐Alexander‐Universität (FAU) Erlangen‐Nürnberg 91052 Erlangen Germany

Funding

Else Kröner-Fresenius-Stiftung · Cancer Research UK

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