DataVXAdvanced SciencePaper
journal article·2024-10Open access

Infrared Spectroscopy of SARS‐CoV‐2 Viral Protein: from Receptor Binding Domain to Spike Protein

Tiziana Mancini, Salvatore Macis, Rosanna Mosetti, Nicole Luchetti, Velia Minicozzi, Andrea Notargiacomo, Marialilia Pea, Augusto Marcelli, Giancarlo Della Ventura, Stefano Lupi, Annalisa D'Arco

Advanced Science· Volume 11· Issue 39
Publisher pageOpen full text
Source abstract

Abstract

Abstract Spike (S) glycoprotein is the largest structural protein of SARS‐CoV‐2 virus and the main one involved in anchoring of the host receptor ACE2 through the receptor binding domain (RBD). S protein secondary structure is of great interest for shedding light on various aspects, from functionality to pathogenesis, finally to spectral fingerprint for the design of optical biosensors. In this paper, the secondary structure of SARS‐CoV‐2 S protein and its constituting components, namely RBD, S1 and S2 regions, are investigated at serological pH by measuring their amide I infrared absorption bands through Attenuated Total Reflection Infrared (ATR‐IR) spectroscopy. Experimental data in combination with MultiFOLD predictions, Define Secondary Structure of Proteins (DSSP) web server and Gravy value calculations, provide a comprehensive understanding of RBD, S1, S2, and S proteins in terms of their secondary structure content, conformational order, and interaction with the solvent.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: SARS-CoV-2 and COVID-19 Research · Spectroscopy Techniques in Biomedical and Chemical Research · SARS-CoV-2 detection and testing

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Physics University La Sapienza P.le A. Moro 2 Rome 00185 Italy

Department of Basic and Applied Sciences for Engineering (SBAI) University La Sapienza Via A. Scarpa 16 Rome 00161 Italy

Engineering Department University Campus Bio‐Medico of Rome Via Alvaro del Portillo 21 Rome 00128 Italy

Centre for Life Nano‐ and Neuro‐Science Italian Institute of Technology Viale Regina Elena 291 Rome 00161 Italy

Department of Physics University of Rome Tor Vergata Via della Ricerca Scientifica 1 Rome 00133 Italy

Istituto di fotonica e nanotecnologie – Consiglio nazionale delle ricerche (CNR‐IFN) Rome 00133 Italy

Laboratori Nazionali Frascati National Institute for Nuclear Physics (INFN‐LNF) Via E. Fermi 54 Frascati 00044 Italy

RICMASS Rome International Center for Materials Science Superstripes Rome 00185 Italy

Department of Science University Rome Tre Largo San Leonardo Murialdo 1 Rome 00146 Italy

References (55)

Show source-provided references
1. Cucinotta D. · Acta Biomed. · 2020 · 91 · 157
2. 10.1038/s41598-021-91950-510.1038/s41598-021-91950-5 ↗
3. 10.1088/1478-3975/abac5110.1088/1478-3975/abac51 ↗
4. 10.1128/JVI.77.16.8801-8811.200310.1128/JVI.77.16.8801-8811.2003 ↗
5. 10.1038/s41423-020-0400-410.1038/s41423-020-0400-4 ↗
6. 10.1007/s12250-020-00212-710.1007/s12250-020-00212-7 ↗
7. 10.1016/j.cell.2020.03.04510.1016/j.cell.2020.03.045 ↗
8. 10.1126/science.abb250710.1126/science.abb2507 ↗
9. 10.1021/acscentsci.0c0105610.1021/acscentsci.0c01056 ↗
10. 10.1038/s41467-020-16567-010.1038/s41467-020-16567-0 ↗
11. 10.1038/s41594-019-0233-y10.1038/s41594-019-0233-y ↗
12. 10.1093/glycob/cwaa04210.1093/glycob/cwaa042 ↗
13. 10.1038/s41591-020-0820-910.1038/s41591-020-0820-9 ↗
14. 10.1073/pnas.170730411410.1073/pnas.1707304114 ↗
15. 10.1038/nsmb.329310.1038/nsmb.3293 ↗
16. 10.1016/S1367-5931(99)00021-610.1016/S1367-5931(99)00021-6 ↗
17. 10.1007/s00018-002-8437-310.1007/s00018-002-8437-3 ↗
18. 10.1007/s00232-015-9802-010.1007/s00232-015-9802-0 ↗
19. 10.1038/s41598-019-39382-010.1038/s41598-019-39382-0 ↗
20. 10.1016/S0079-6107(00)00021-310.1016/S0079-6107(00)00021-3 ↗
21. 10.3390/molecules2401018610.3390/molecules24010186 ↗
22. 10.3390/app1102054010.3390/app11020540 ↗
23. 10.3390/ijms2411955010.3390/ijms24119550 ↗
24. 10.1016/j.ijbiomac.2022.08.19710.1016/j.ijbiomac.2022.08.197 ↗
25. 10.1016/j.jviromet.2014.09.01310.1016/j.jviromet.2014.09.013 ↗
26. Kim K. · Environ. Sci. Technol. · 2021 · 55
27. 10.1093/nar/gkad29710.1093/nar/gkad297 ↗
28. 10.3390/nano1105110310.3390/nano11051103 ↗
29. 10.1038/nprot.2015.02410.1038/nprot.2015.024 ↗
30. 10.1016/j.saa.2005.08.02510.1016/j.saa.2005.08.025 ↗
31. 10.1021/acs.jpcb.7b0682610.1021/acs.jpcb.7b06826 ↗
32. 10.1021/jz500794d10.1021/jz500794d ↗
33. 10.1016/j.msec.2012.08.04410.1016/j.msec.2012.08.044 ↗
34. 10.1002/bip.36022121110.1002/bip.360221211 ↗
35. 10.1093/nar/gku102810.1093/nar/gku1028 ↗
36. 10.1038/nrm244610.1038/nrm2446 ↗
37. 10.1111/j.1745-7270.2007.00320.x10.1111/j.1745-7270.2007.00320.x ↗
38. 10.1080/10408398.2021.187158910.1080/10408398.2021.1871589 ↗
39. 10.1016/j.bbamem.2013.04.00810.1016/j.bbamem.2013.04.008 ↗
40. 10.3390/biom1208107110.3390/biom12081071 ↗
41. 10.1016/j.coviro.2021.08.01010.1016/j.coviro.2021.08.010 ↗
42. 10.1038/s41564-020-0688-y10.1038/s41564-020-0688-y ↗
43. 10.1021/acschemneuro.0c0064210.1021/acschemneuro.0c00642 ↗
44. 10.1063/1.140784210.1063/1.1407842 ↗
45. 10.1017/S003358350200381510.1017/S0033583502003815 ↗
46. 10.1021/jp047120410.1021/jp0471204 ↗
47. 10.1021/jp053956a10.1021/jp053956a ↗
48. 10.1002/bip.36025040910.1002/bip.360250409 ↗
49. 10.1021/ja001782z10.1021/ja001782z ↗
50. 10.1021/jp805735510.1021/jp8057355 ↗
51. 10.1073/pnas.190506811610.1073/pnas.1905068116 ↗
52. 10.1006/abio.1999.432010.1006/abio.1999.4320 ↗
53. 10.1007/s00249-021-01507-710.1007/s00249-021-01507-7 ↗
54. 10.1093/bioinformatics/btt47310.1093/bioinformatics/btt473 ↗
55. 10.1002/prot.2026410.1002/prot.20264 ↗