journal article·2024-06Open access

Robotic Actuation‐Mediated Quantitative Mechanogenetics for Noninvasive and On‐Demand Cancer Therapy

Yangyi Liu, Jingjing Li, Yi Zhang, Fan Wang, Juanjuan Su, Chao Ma, Shuyi Zhang, Yanan Du, Chunhai Fan, Hongjie Zhang, Kai Liu

Advanced Science· Volume 11· Issue 23

Abstract

Abstract Cell mechanotransduction signals are important targets for physical therapy. However, current physiotherapy heavily relies on ultrasound, which is generated by high‐power equipment or amplified by auxiliary drugs, potentially causing undesired side effects. To address current limitations, a robotic actuation‐mediated therapy is developed that utilizes gentle mechanical loads to activate mechanosensitive ion channels. The resulting calcium influx precisely regulated the expression of recombinant tumor suppressor protein and death‐associated protein kinase, leading to programmed apoptosis of cancer cell line through caspase‐dependent pathway. In stark contrast to traditional gene therapy, the complete elimination of early‐ and middle‐stage tumors (volume ≤ 100 mm 3 ) and significant growth inhibition of late‐stage tumor (500 mm 3 ) are realized in tumor‐bearing mice by transfecting mechanogenetic circuits and treating daily with quantitative robotic actuation in a form of 5 min treatment over the course of 14 days. Thus, this massage‐derived therapy represents a quantitative strategy for cancer treatment.
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Research context

OpenAlex topics: Cellular Mechanics and Interactions · Erythrocyte Function and Pathophysiology · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Center of Materials Science and Optoelectronics Engineering College of Materials Science and Optoelectronic Technology University of Chinese Academy of Sciences Beijing 100049 China

Engineering Research Center of Advanced Rare Earth Materials (Ministry of Education) Department of Chemistry Tsinghua University Beijing 100084 China

State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

School of Pharmaceutical Sciences Tsinghua University Beijing 100084 China

Department of Biomedical Engineering School of Medicine Tsinghua‐Peking Center for Life Sciences Tsinghua University Beijing 100084 China

Xiangfu Laboratory Jiaxing 314102 China

School of Chemistry and Chemical Engineering New Cornerstone Science Laboratory Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study and National Center for Translational Medicine Shanghai Jiao Tong University Shanghai 200240 China

Funding

National Natural Science Foundation of China · Beijing Nova Program · Youth Innovation Promotion Association of the Chinese Academy of Sciences · Natural Science Foundation of Beijing Municipality · Key Technologies Research and Development Program

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