DataVXAdvanced SciencePaper
journal article·2023-08Open access

Quantification of Surface Tension Effects and Nucleation‐and‐Growth Rates during Self‐Assembly of Biological Condensates

Zsuzsa Sárkány, Fernando Rocha, Anna Bratek‐Skicki, Peter Tompa, Sandra Macedo‐Ribeiro, Pedro M. Martins

Advanced Science· Volume 10· Issue 23
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Abstract

Abstract Liquid‐solid and liquid‐liquid phase separation (PS) drives the formation of functional and disease‐associated biological assemblies. Principles of phase equilibrium are here employed to derive a general kinetic solution that predicts the evolution of the mass and size of biological assemblies. Thermodynamically, protein PS is determined by two measurable concentration limits: the saturation concentration and the critical solubility. Due to surface tension effects, the critical solubility can be higher than the saturation concentration for small, curved nuclei. Kinetically, PS is characterized by the primary nucleation rate constant and a combined rate constant accounting for growth and secondary nucleation. It is demonstrated that the formation of a limited number of large condensates is possible without active mechanisms of size control and in the absence of coalescence phenomena. The exact analytical solution can be used to interrogate how the elementary steps of PS are affected by candidate drugs.
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Research context

OpenAlex topics: RNA Research and Splicing · thermodynamics and calorimetric analyses · Protein Structure and Dynamics

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

IBMC − Instituto de Biologia Molecular e Celular Universidade do Porto Porto 4150–180 Portugal

i3S − Instituto de Investigação e Inovação em Saúde Universidade do Porto Porto 4150–180 Portugal

LEPABE − Laboratory for Process Engineering Environment Biotechnology and Energy Faculdade de Engenharia da Universidade do Porto Porto 4200‐465 Portugal

Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences Niezapominajek 8 Krakow PL30239 Poland

VIB‐VUB Center for Structural Biology Vlaams Instituut voor Biotechnology Brussels 1050 Ixelles Belgium

Structural Biology Brussels (SBB) Bioengineering Sciences Department Vrije Universiteit Brussel (VUB) Brussels B‐1050 Belgium

Institute of Enzymology Research Centre for Natural Sciences Budapest 1117 Hungary

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