A Bidirectional Single‐Cell Migration and Retrieval Chip for Quantitative Study of Dendritic Cell Migration
Source abstract
Abstract
Abstract Dendritic cell (DC) migration is a fundamental step during execution of its adaptive immunity functions. Studying DC migration characteristics is critical for development of DC‐dependent allergy treatments, vaccines, and cancer immunotherapies. Here, a microfluidics‐based single‐cell migration platform is described that enables high‐throughput and precise bidirectional cell migration assays. It also allows selective retrieval of cell subpopulations that have different migratory potentials. Using this microfluidic platform, DC migration is investigated in response to different chemoattractants and inhibitors, quantitatively describe DC migration patterns and retrieve DC subpopulations of different migratory potentials for differential gene expression analysis. This platform opens an avenue for precise characterization of cell migration and potential discovery of therapeutic modulators.
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Research context
OpenAlex topics: Immunotherapy and Immune Responses · RNA Interference and Gene Delivery · T-cell and B-cell Immunology
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Nanomedicine Houston Methodist Research Institute Houston TX 77030 USA
The Third Xiangya Hospital Central South University Changsha 410008 P. R. China
Department of Molecular and Cellular Oncology The University of Texas MD Anderson Cancer Center Houston TX 77030 USA
Department of Breast Surgery Harbin Medical University Cancer Hospital Harbin 150081 P. R. China
Department of Genetics The University of Texas MD Anderson Cancer Center Houston TX 77030 USA
Funding
National Institutes of Health
References (60)
Show source-provided references
1. 10.4161/cc.8.23.999810.4161/cc.8.23.9998 ↗
2. 10.1038/nri.2016.11610.1038/nri.2016.116 ↗
3. 10.1038/s41423-021-00726-410.1038/s41423-021-00726-4 ↗
4. 10.1016/j.critrevonc.2016.09.00210.1016/j.critrevonc.2016.09.002 ↗
5. 10.1016/j.immuni.2010.04.01710.1016/j.immuni.2010.04.017 ↗
6. 10.1126/science.122845610.1126/science.1228456 ↗
7. 10.4049/jimmunol.100235810.4049/jimmunol.1002358 ↗
8. 10.1038/s41423-018-0005-310.1038/s41423-018-0005-3 ↗
9. 10.1016/S0140-6736(10)61226-310.1016/S0140-6736(10)61226-3 ↗
10. 10.1016/j.immuni.2013.07.00410.1016/j.immuni.2013.07.004 ↗
11. 10.1038/s41577-019-0210-z10.1038/s41577-019-0210-z ↗
12. 10.1084/jem.115.3.45310.1084/jem.115.3.453 ↗
13. 10.1083/jcb.75.2.60610.1083/jcb.75.2.606 ↗
14. 10.1242/jcs.99.4.76910.1242/jcs.99.4.769 ↗
15. 10.1242/jcs.52.1.110.1242/jcs.52.1.1 ↗
16. 10.1073/pnas.101492010810.1073/pnas.1014920108 ↗
17. 10.1038/ncomms578710.1038/ncomms5787 ↗
18. 10.1038/srep0998010.1038/srep09980 ↗
19. 10.1038/srep3644010.1038/srep36440 ↗
20. 10.1002/smtd.20170025410.1002/smtd.201700254 ↗
21. 10.1021/acs.analchem.9b0368110.1021/acs.analchem.9b03681 ↗
22. 10.3389/fimmu.2019.0035910.3389/fimmu.2019.00359 ↗
23. 10.1039/b710524j10.1039/b710524j ↗
24. 10.1126/science.115989410.1126/science.1159894 ↗
25. 10.1371/journal.pone.001818310.1371/journal.pone.0018183 ↗
26. 10.1016/j.jim.2015.12.00510.1016/j.jim.2015.12.005 ↗
27. 10.1038/ncb328410.1038/ncb3284 ↗
28. 10.1126/sciimmunol.aak957310.1126/sciimmunol.aak9573 ↗
29. 10.3390/mi1012085110.3390/mi10120851 ↗
30. 10.1073/pnas.131366111110.1073/pnas.1313661111 ↗
31. 10.1039/C5IB00200A10.1039/C5IB00200A ↗
32. 10.1038/ni.f.21210.1038/ni.f.212 ↗
33. 10.1126/science.aba289410.1126/science.aba2894 ↗
34. 10.1002/0471142735.im0732s9910.1002/0471142735.im0732s99 ↗
35. 10.1038/nature0688710.1038/nature06887 ↗
36. 10.1111/imr.1210810.1111/imr.12108 ↗
37. 10.4049/jimmunol.155.7.329210.4049/jimmunol.155.7.3292 ↗
38. 10.1007/s12195-014-0342-y10.1007/s12195-014-0342-y ↗
39. 10.3389/fimmu.2013.0035610.3389/fimmu.2013.00356 ↗
40. 10.3389/fphar.2022.84168710.3389/fphar.2022.841687 ↗
41. 10.1189/jlb.1A0314-170R10.1189/jlb.1A0314-170R ↗
42. 10.1182/blood-2011-10-38340610.1182/blood-2011-10-383406 ↗
43. 10.1182/blood-2008-11-18930810.1182/blood-2008-11-189308 ↗
44. 10.3389/fimmu.2020.0052810.3389/fimmu.2020.00528 ↗
45. 10.4049/jimmunol.174.7.407010.4049/jimmunol.174.7.4070 ↗
46. 10.4049/jimmunol.140196610.4049/jimmunol.1401966 ↗
47. 10.1074/jbc.M50823420010.1074/jbc.M508234200 ↗
48. 10.1016/j.cellimm.2011.09.01510.1016/j.cellimm.2011.09.015 ↗
49. 10.1124/mol.108.05338910.1124/mol.108.053389 ↗
50. 10.1089/scd.2017.019110.1089/scd.2017.0191 ↗
51. 10.1039/C4LC01038H10.1039/C4LC01038H ↗
52. 10.1038/s41551-019-0400-910.1038/s41551-019-0400-9 ↗
53. 10.1002/anie.20190369410.1002/anie.201903694 ↗
54. 10.1038/nprot.2008.21110.1038/nprot.2008.211 ↗
55. 10.1093/nar/gkac19410.1093/nar/gkac194 ↗
56. 10.1146/annurev-cellbio-020520-12062710.1146/annurev-cellbio-020520-120627 ↗
57. 10.1126/science.aad729710.1126/science.aad7297 ↗
58. 10.1126/science.aad761110.1126/science.aad7611 ↗
59. 10.1083/jcb.20190111610.1083/jcb.201901116 ↗
60. 10.1016/j.ceb.2020.01.01110.1016/j.ceb.2020.01.011 ↗