Hydrogel‐Transformable Probiotic Powder for Targeted Eradication of Helicobacter pylori with Enhanced Gastric Mucosal Repair and Microbiota Preservation

Abstract Lactobacillus reuteri ( L. reuteri ) therapies represent a potentially effective approach to eradicating Helicobacter pylori (H. pylori) . However, the difficulty in bacterial viability preservation and harsh gastric environment compromises the survival and on‐target delivery of L. reuteri . This study presents a novel bacterium‐mediated bacterial elimination strategy using an edible L. reuteri @HTP probiotic powder for targeted bacterial elimination. The probiotic powder is obtained by grinding a lyophilized hydrogel composed of L. reuteri , hyaluronic acid (HA), tannic acid (TA), and polyvinyl alcohol (PVA). Upon contact with water, the powder quickly transforms into a hydrogel, enhancing L. reuteri ’s survival in the harsh gastric environment and ensuring selective release at H. pylori ‐infected inflammatory sites. L. reuteri targets and reduces H. pylori colonization while secreting reuterin to eliminate the bacteria. Additionally, TA's antioxidant properties help alleviate inflammation, and HA supports gastric mucosal repair. L. reuteri @HTP powder preserves the integrity of the gut microbiota, facilitating the restoration of a healthy microbiome. In particular, the probiotic powder remains stable at room temperature for at least six months, providing a promising alternative to traditional antibiotics for H. pylori treatment. This strategy combines targeted eradication, mucosal healing, and microbiome restoration, offering a new approach to treating gastric infections.