DataVXAdvanced SciencePaper
journal article·2024-12Open access

Quantum Dots‐caused Retinal Degeneration in Zebrafish Regulated by Ferroptosis and Mitophagy in Retinal Pigment Epithelial Cells through Inhibiting Spliceosome

Naying Zheng, Tingting Liao, Chuchu Zhang, Zheyang Zhang, Sen Yan, Xiaohan Xi, Fengkai Ruan, Chunyan Yang, Qingliang Zhao, Wenbo Deng, Jialiang Huang, Zi‐Tao Huang, Zhi‐Feng Chen, Xiang Wang, Qingming Qu, Zhenghong Zuo, Chengyong He

Advanced Science· Volume 11· Issue 46
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Source abstract

Abstract

Abstract Quantum dots (QDs) are widely used, but their health impact on the visual system is little known. This study aims to elucidate the effects and mechanisms of typical metallic QDs on retinas using zebrafish. Comprehensive histology, imaging, and bulk RNA sequencing reveal that InP/ZnS QDs cause retinal degeneration. Furthermore, single‐cell RNA‐seq reveals a reduction in the number of retinal pigment epithelial cells (RPE) and short‐wave cone UV photoreceptor cells (PR(UV)), accompanied by an increase in middle‐ and long‐wave cone red, green, and blue photoreceptor cells [PR(RGB)]. Mechanistically, after endocytosis by RPE, InP/ZnS QDs inhibit the expression of splicing factor prpf8, resulting in gpx4b mRNA unsplicing, which finally decrease glutathione and induce ferroptosis and mitophagy. The decrease of RPE fails to engulf the damaged outer segments of PR, possibly promoting the differentiation of PR(UV) to PR(RGB). Knockout prpf8 or gpx4b with CRISPR/Cas9 system, the retinal damage is also observed. Whereas, overexpression of prpf8 or gpx4b, or supplement of glutathione can rescue the retinal degenerative damage caused by InP/ZnS QDs. In conclusion, this study illustrates the potential health risks of InP/ZnS QDs on eye development and provides valuable insights into the underlying mechanisms of InP/ZnS QDs‐caused retinal degeneration.
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Research context

OpenAlex topics: Extracellular vesicles in disease · Advanced biosensing and bioanalysis techniques · Retinal Development and Disorders

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Ophthalmology in Xiang'an Hospital of Xiamen University State Key Laboratory of Cellular Stress Biology School of Life Sciences Faculty of Medicine and Life Sciences Xiamen University Xiamen Fujian 361102 China

Department of Chemistry State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials (i‐ChEM) Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM) College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 China

State Key Laboratory of Vaccines for Infectious Diseases Center for Molecular Imaging and Translational Medicine Xiang An Biomedicine Laboratory School of Public Health Xiamen University Xiamen Fujian 361005 China

Key Laboratory of Reproductive Health Research Fujian Province University School of Medicine Xiamen University Xiamen Fujian 361005 China

Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control Guangdong‐Hong Kong‐Macao Joint Laboratory for Contaminants Exposure and Health School of Environmental Science and Engineering Guangdong University of Technology Guangzhou 510006 China

Funding

National Natural Science Foundation of China · Fundamental Research Funds for the Central Universities

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