DataVXAdvanced SciencePaper
journal article·2025-06Open access

Microneedle Patch for In Situ Neutrophil Monitoring

Ziyi Lu, Ruisi Cai, Tianao Xie, Sheng Zhao, Sarun Juengpanich, Win Topatana, Shijie Li, Jiasheng Cao, Jiahao Hu, Tianen Chen, Jiachen Chen, Jicheng Yu, Tao Sheng, Wentao Zhang, Hao Wang, Jiahuan You, Yukai Shan, Yuchao Sun, Ruijing Shen, Zhengjie Zhao, Kangfan Ji, Ziqi Gao, Xinmin Yu, Xiujun Cai, Zhen Gu, Yuqi Zhang, Mingyu Chen

Advanced Science· Volume 12· Issue 23
Publisher pageOpen full text
Source abstract

Abstract

Abstract Monitoring neutrophil levels plays an essential role in diagnosing infections and various diseases, as well as for managing specific treatments such as chemotherapy and radiotherapy. However, the traditional complete blood count technique is invasive and challenging for quick and frequent monitoring of neutrophil. Herein, a button‐like microneedle patch for rapid and convenient detection of neutrophil levels is reported without vascular invasion. This patch consists of a hollow microneedle array with a vacuum chamber for extraction of interstitial fluid, a hydrogel sheet for colorimetric detection of neutrophil level based on neutrophil‐specific enzyme (myeloperoxidase), and a mobile phone application for data recording and quantification. The detection ability is validated both in vitro and in vivo in a chemotherapy‐induced neutropenic rat model. Moreover, this button patch achieves precise measurement of the neutrophil levels in patients’ urine and abdominal drainage fluid. This minimally invasive colorimetric patch can potentiate in situ neutrophil monitoring without blood sampling procedures or in‐lab apparatus, which can serve as a substitution for routine blood counts especially for patients in less developed areas.
Source: crossref · Quality: baseline

Research context

Polymers

OpenAlex topics: Advancements in Transdermal Drug Delivery · Lipid Membrane Structure and Behavior · Protein Interaction Studies and Fluorescence Analysis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of General Surgery, Sir Run Run Shaw Hospital Zhejiang University Hangzhou 310016 China

State Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang Provincial Key Laboratory for Advanced Drug Delivery Systems, College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China

National Engineering Research Center of Innovation and Application of Minimally Invasive Instruments Zhejiang University Hangzhou 310000 China

Jinhua Institute of Zhejiang University Jinhua 321299 China

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering Zhejiang University Hangzhou 310027 China

Institute of Fundamental and Transdisciplinary Research Zhejiang University Hangzhou 310058 China

Department of Burns and Wound Care Center, Second Affiliated Hospital, School of Medicine Zhejiang University Hangzhou 310009 China

Funding

National Natural Science Foundation of China

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