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journal article·2024-10Open access

General Post‐Regulation Strategy of AIEgens’ Photophysical Properties for Intravital Two‐Photon Fluorescence Imaging

Liyun Lin, Jiaxin Liu, Zhengyuan Pan, Wen Pang, Xinyan Jiang, Man Lei, Jucai Gao, Yujie Xiao, Bo Li, Fang Hu, Zhouzhou Bao, Xunbin Wei, Wenbo Wu, Bobo Gu

Advanced Science· Volume 11· Issue 38
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Source abstract

Abstract

Abstract Fluorogens with aggregation‐induced emission (AIEgens) are promising agents for two‐photon fluorescence (TPF) imaging. However, AIEgens’ photophysical properties are fixed and unoptimizable once synthesized. Therefore, it is urgent and meaningful to explore an efficient post‐regulation strategy to optimize AIEgens’ photophysical properties. Herein, a general and efficient post‐regulation strategy is reported. By simply tuning the ratio of inert AIEgens within binary nanoparticles (BNPs), the fluorescence quantum yield and two‐photon absorption cross‐section of functional AIEgens are enhanced by 8.7 and 5.4 times respectively, which are not achievable by conventional strategies, and the notorious phototoxicity is almost eliminated. The experimental results, theoretical simulation, and mechanism analysis demonstrated its feasibility and generality. The BNPs enabled deep cerebrovascular network imaging with ≈1.10 mm depth and metastatic cancer cell detection with single‐cell resolution. Furthermore, the TPF imaging quality is improved by the self‐supervised denoising algorithm. The proposed binary molecular post‐regulation strategy opened a new avenue to efficiently boost the AIEgens’ photophysical properties and consequently TPF imaging quality.
Source: crossref · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Nanoplatforms for cancer theranostics · Luminescence and Fluorescent Materials · Nanocluster Synthesis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 China

Department of Chemistry Institute of Molecular Aggregation Science Tianjin University Tianjin 300072 China

Biomaterials Research Center School of Biomedical Engineering Southern Medical University Guangzhou 510515 China

Department of Neurology Huashan Hospital MOE Frontiers Center for Brain Science State Key Laboratory of Medical Neurobiology Institutes for Translational Brain Research Fudan University Shanghai 200437 China

Shanghai Key Laboratory of Gynecologic Oncology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200127 China

Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing) Peking University Cancer Hospital & Institute Beijing 100142 China

Biomedical Engineering Department and International Cancer Institute Peking University Beijing 100191 China

Funding

National Key Research and Development Program of China · National Natural Science Foundation of China · Shanghai Rising-Star Program · Natural Science Foundation of Shanghai Municipality · Shanghai Jiao Tong University

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