Chromatin Modifier EP400 Regulates Oocyte Quality and Zygotic Genome Activation in Mice
Source abstract
Abstract
Abstract Epigenetic modifiers that accumulate in oocytes, play a crucial role in steering the developmental program of cleavage embryos and initiating life. However, the identification of key maternal epigenetic regulators remains elusive. In the findings, the essential role of maternal Ep400 , a chaperone for H3.3, in oocyte quality and early embryo development in mice is highlighted. Depletion of Ep400 in oocytes resulted in a decline in oocyte quality and abnormalities in fertilization. Preimplantation embryos lacking maternal Ep400 exhibited reduced major zygotic genome activation (ZGA) and experienced developmental arrest at the 2‐to‐4‐cell stage. The study shows that EP400 forms protein complex with NFYA, occupies promoters of major ZGA genes, modulates H3.3 distribution between euchromatin and heterochromatin, promotes transcription elongation, activates the expression of genes regulating mitochondrial functions, and facilitates the expression of rate‐limiting enzymes of the TCA cycle. This intricate process driven by Ep400 ensures the proper execution of the developmental program, emphasizing its critical role in maternal‐to‐embryonic transition.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Epigenetics and DNA Methylation · MicroRNA in disease regulation · RNA Research and Splicing
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Reproductive Health Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China
Department of Gynecology and Obstetrics Zhongnan Hospital of Wuhan University Wuhan Hubei 430071 China
Department of Physiology School of Basic Medicine, Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China
Center for Genomics and Proteomics Research School of Basic Medicine, Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China
Hubei Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation Huazhong University of Science and Technology Wuhan Hubei 430030 China
Department of Biochemistry Osaka Medical and Pharmaceutical University Takatsuki Osaka 569‐1094 Japan
State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China
Funding
National Natural Science Foundation of China · National Basic Research Program of China
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