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journal article·2024-12Open access

3D Micropatterned Traction Force Microscopy: A Technique to Control 3D Cell Shape While Measuring Cell‐Substrate Force Transmission

Laura M. Faure, Manuel Gómez‐González, Ona Baguer, Jordi Comelles, Elena Martínez, Marino Arroyo, Xavier Trepat, Pere Roca‐Cusachs

Advanced Science· Volume 11· Issue 46
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Abstract

Abstract Cell shape and function are intimately linked, in a way that is mediated by the forces exerted between cells and their environment. The relationship between cell shape and forces has been extensively studied for cells seeded on flat 2D substrates, but not for cells in more physiological 3D settings. Here, a technique called 3D micropatterned traction force microscopy (3D‐µTFM) to confine cells in 3D wells of defined shape, while simultaneously measuring the forces transmitted between cells and their microenvironment is demonstrated. This technique is based on the 3D micropatterning of polyacrylamide wells and on the calculation of 3D traction force from their deformation. With 3D‐µTFM, it is shown that MCF10A breast epithelial cells exert defined, reproducible patterns of forces on their microenvironment, which can be both contractile and extensile. Cells switch from a global contractile to extensile behavior as their volume is reduced are further shown. The technique enables the quantitative study of cell mechanobiology with full access to 3D cellular forces while having accurate control over cell morphology and the mechanical conditions of the microenvironment.
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Research context

OpenAlex topics: Cellular Mechanics and Interactions · Microfluidic and Bio-sensing Technologies · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute for Bioengineering of Catalonia (IBEC) Barcelona Institute of Science and Technology (BIST) C. Baldiri Reixac 10‐12 Barcelona 08028 Spain

Department of Biomedical Sciences University of Barcelona C. Casanova 143 Barcelona 08034 Spain

Department of Electronics and Biomedical Engineering University of Barcelona C. Martí Franquès 1 Barcelona 08028 Spain

Centro de Investigación Biomédica en Red en Bioingeniería Biomateriales y Nanomedicina (CIBER‐BBN) Avenida Monforte de Lemos 3‐5 Madrid 28029 Spain

Laboratori de Càlcul Numèric (LaCàN) Universitat Politècnica de Catalunya (UPC) Jordi Girona 1‐3 Barcelona 08036 Spain

Institut de Matemàtiques de la UPC–BarcelonaTech (IMTech) Pau Gargallo 14 Barcelona 08028 Spain

Centre Internacional de Mètodes Numèrics en Enginyeria (CIMNE) Gran Capità S/N Barcelona 08034 Spain

Institució Catalana de Recerca i Estudis Avançats (ICREA) Lluis Companys 23 Barcelona 08010 Spain

Funding

Fundación Bancaria Caixa d'Estalvis i Pensions de Barcelona · H2020 European Research Council · Horizon 2020 · EMBO · Ministerio de Ciencia e Innovación · European Research Council · Generalitat de Catalunya · Fundació la Marató de TV3

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