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journal article·2024-04Open access

Live Organoid Cyclic Imaging

David E. Reynolds, Yusha Sun, Xin Wang, Phoebe Vallapureddy, Jianhua Lim, Menghan Pan, Andres Fernandez Del Castillo, Jonathan C. T. Carlson, Mark A. Sellmyer, MacLean Nasrallah, Zev Binder, Donald M. O'Rourke, Guo‐li Ming, Hongjun Song, Jina Ko

Advanced Science· Volume 11· Issue 14
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Source abstract

Abstract

Abstract Organoids are becoming increasingly relevant in biology and medicine for their physiological complexity and accuracy in modeling human disease. To fully assess their biological profile while preserving their spatial information, spatiotemporal imaging tools are warranted. While previously developed imaging techniques, such as four‐dimensional (4D) live imaging and light‐sheet imaging have yielded important clinical insights, these technologies lack the combination of cyclic and multiplexed analysis. To address these challenges, bioorthogonal click chemistry is applied to display the first demonstration of multiplexed cyclic imaging of live and fixed patient‐derived glioblastoma tumor organoids. This technology exploits bioorthogonal click chemistry to quench fluorescent signals from the surface and intracellular of labeled cells across multiple cycles, allowing for more accurate and efficient molecular profiling of their complex phenotypes. Herein, the versatility of this technology is demonstrated for the screening of glioblastoma markers in patient‐derived human glioblastoma organoids while conserving their viability. It is anticipated that the findings and applications of this work can be broadly translated into investigating physiological developments in other organoid systems.
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Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Click Chemistry and Applications · RNA Interference and Gene Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Bioengineering University of Pennsylvania Philadelphia PA 19104 USA

Department of Neuroscience Mahoney Institute for Neurosciences Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA

Department of Biochemistry & Molecular Biophysics Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA

Center for Systems Biology Massachusetts General Hospital Boston MA 02114 USA

Department of Medicine Massachusetts General Hospital Harvard Medical School Boston MA 02114 USA

Department of Radiology Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA

GBM Translational Center of Excellence Abramson Cancer Center University of Pennsylvania Philadelphia PA 19104 USA

Department of Pathology and Laboratory Medicine University of Pennsylvania Philadelphia PA 19104 USA

Center for Cellular Immunotherapies University of Pennsylvania Philadelphia PA 19104 USA

Department of Neurosurgery Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA

Department of Cell and Developmental Biology Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA

Department of Psychiatry Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA

Institute for Regenerative Medicine University of Pennsylvania Philadelphia PA 19104 USA

The Epigenetics Institute Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA

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