DataVXAdvanced SciencePaper
journal article·2023-08Open access

Direct Quantitation of SARS‐CoV‐2 Virus in Urban Ambient Air via a Continuous‐Flow Electrochemical Bioassay

Fuze Jiang, Bei Liu, Yang Yue, Yile Tao, Zhen Xiao, Meng Li, Zheng Ji, Jiukai Tang, Guangyu Qiu, Martin Spillmann, Junji Cao, Lianjun Zhang, Jing Wang

Advanced Science· Volume 10· Issue 22
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Abstract

Abstract Airborne SARS‐CoV‐2 virus surveillance faces challenges in complicated biomarker enrichment, interferences from various non‐specific matters and extremely low viral load in the urban ambient air, leading to difficulties in detecting SARS‐CoV‐2 bioaerosols. This work reports a highly specific bioanalysis platform, with an exceptionally low limit‐of‐detection (≤1 copy m−3) and good analytical accordance with RT‐qPCR, relying on surface‐mediated electrochemical signaling and enzyme‐assisted signal amplification, enabling gene and signal amplification for accurate identification and quantitation of low doses human coronavirus 229E (HCoV‐229E) and SARS‐CoV‐2 viruses in urban ambient air. This work provides a laboratory test using cultivated coronavirus to simulate the airborne spread of SARS‐CoV‐2, and validate that the platform could reliably detect airborne coronavirus and reveal the transmission characteristics. This bioassay conducts the quantitation of real‐world HCoV‐229E and SARS‐CoV‐2 in airborne particulate matters collected from road‐side and residential areas in Bern and Zurich (Switzerland) and Wuhan (China), with resultant concentrations verified by RT‐qPCR.
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Research context

OpenAlex topics: SARS-CoV-2 detection and testing · Biosensors and Analytical Detection · SARS-CoV-2 and COVID-19 Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Environmental Engineering ETH Zürich Zürich CH‐8093 Switzerland

Advanced Analytical Technologies Empa Dübendorf CH‐8600 Switzerland

Guangzhou Laboratory Guangzhou International Bio Island Guangzhou Guangdong 510005 China

School of Environment Harbin Institute of Technology Harbin 150090 China

Institute of Systems Medicine Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100005 China

Suzhou Institute of Systems Medicine Suzhou 215123 China

Zurich Instruments AG Zürich CH‐8005 Switzerland

School of Geography and Tourism Shaanxi Normal University Xi'an 710119 China

International Joint Research Centre of Shaanxi Province for Pollutant Exposure and Eco‐Environmental Health Xi'an 710062 China

Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung · National Natural Science Foundation of China · China Scholarship Council

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