DataVXAdvanced SciencePaper
journal article·2023-03Open access

Real‐Time Dissection of the Transportation and miRNA‐Release Dynamics of Small Extracellular Vesicles

Hou‐Fu Xia, Zi‐Li Yu, Li‐Juan Zhang, Shu‐Lin Liu, Yi Zhao, Jue Huang, Dan‐Dan Fu, Qi‐Hui Xie, Hai‐Ming Liu, Zhi‐Ling Zhang, Yi‐Fang Zhao, Min Wu, Wei Zhang, Dai‐Wen Pang, Gang Chen

Advanced Science· Volume 10· Issue 7
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Source abstract

Abstract

Abstract Extracellular vesicles (EVs) are cell‐derived membrane‐enclosed structures that deliver biomolecules for intercellular communication. Developing visualization methods to elucidate the spatiotemporal dynamics of EVs’ behaviors will facilitate their understanding and translation. With a quantum dot (QD) labeling strategy, a single particle tracking (SPT) platform is proposed here for dissecting the dynamic behaviors of EVs. The interplays between tumor cell‐derived small EVs (T‐sEVs) and endothelial cells (ECs) are specifically investigated based on this platform. It is revealed that, following a clathrin‐mediated endocytosis by ECs, T‐sEVs are transported to the perinuclear region in a typical three‐stage pattern. Importantly, T‐sEVs frequently interact with and finally enter lysosomes, followed by quick release of their carried miRNAs. This study, for the first time, reports the entire process and detailed dynamics of T‐sEV transportation and cargo‐release in ECs, leading to better understanding of their proangiogenic functions. Additionally, the QD‐based SPT technique will help uncover more secrets of sEV‐mediated cell–cell communication.
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Research context

OpenAlex topics: Extracellular vesicles in disease · MicroRNA in disease regulation · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei‐MOST) and Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan 430079 P. R. China

Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Wuhan University Wuhan 430079 P. R. China

College of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 P. R. China

State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratory of Biosensing and Molecular Recognition Research Center for Analytical Sciences and College of Chemistry Nankai University Tianjin 300071 P. R. China

Department of Prosthodontics School and Hospital of Stomatology Wuhan University Wuhan 430079 P. R. China

TaiKang Center for Life and Medical Sciences Wuhan University Wuhan 430071 P. R. China

Frontier Science Center for Immunology and Metabolism Wuhan University Wuhan 430071 P. R. China

Funding

National Natural Science Foundation of China

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