Under‐Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle‐Based Approach for Microscale Cell Culture
Source abstract
Abstract
Abstract Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply–demand balance of the system. Numerical simulation and experimental validation of oxygen transport within multi‐liquid‐phase, microscale culture systems involving a variety of cell types, including mammalian, fungal, and bacterial cells are presented. Finally, AROM is applied to establish a coculture between cells with disparate oxygen demands—primary intestinal epithelial cells (oxygen consuming) and Bacteroides uniformis (an anaerobic species prevalent in the human gut).
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Research context
OpenAlex topics: 3D Printing in Biomedical Research · Innovative Microfluidic and Catalytic Techniques Innovation · Microfluidic and Capillary Electrophoresis Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Carbone Cancer Center University of Wisconsin‐Madison Madison WI 53705 USA
Department of Biomedical Engineering University of Wisconsin‐Madison Madison WI 53705 USA
Department of Biomedical Engineering University of Mississippi University Madison MS 38677 USA
Department of Surgery University of California San Francisco San Francisco CA 94143 USA
Department of Biochemistry University of Wisconsin‐Madison Madison WI 53706 USA
Department of Chemical and Biological Engineering University of Wisconsin‐Madison Madison WI 53706 USA
Analytical Instrumentation Center‐Mass Spec Facility School of Pharmacy University of Wisconsin‐Madison Madison WI 53705 USA
School of Medicine and Public Health University of Wisconsin‐Madison Madison WI 53726 USA
Department of Bacteriology University of Wisconsin‐Madison Madison WI 53706 USA
Department of Pathology and Laboratory Medicine University of Wisconsin‐Madison Madison WI 53705 USA
Funding
National Science Foundation · American Cancer Society
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