journal article·2023-08Open access

Efficient Metabolic Fingerprinting of Follicular Fluid Encodes Ovarian Reserve and Fertility

Jiao Wu, Chunmei Liang, Xin Wang, Yida Huang, Wanshan Liu, Ruimin Wang, Jing Cao, Xun Su, Tao Yin, Xiaolei Wang, Zhikang Zhang, Lingchao Shen, Danyang Li, Weiwei Zou, Ji Wu, Lihua Qiu, Wen Di, Yunxia Cao, Dongmei Ji, Kun Qian

Advanced Science· Volume 10· Issue 23

Abstract

Abstract Ovarian reserve (OR) and fertility are critical in women's healthcare. Clinical methods for encoding OR and fertility rely on the combination of tests, which cannot serve as a multi‐functional platform with limited information from specific biofluids. Herein, metabolic fingerprinting of follicular fluid (MFFF) from follicles is performed, using particle‐assisted laser desorption/ionization mass spectrometry (PALDI‐MS) to encode OR and fertility. PALDI‐MS allows efficient MFFF, showing fast speed (≈30 s), high sensitivity (≈60 fmol), and desirable reproducibility (coefficients of variation <15%). Further, machine learning of MFFF is applied to diagnose diminished OR (area under the curve of 0.929) and identify high‐quality oocytes/embryos (p < 0.05) by a single PALDI‐MS test. Meanwhile, metabolic biomarkers from MFFF are identified, which also determine oocyte/embryo quality (p < 0.05) from the sampling follicles toward fertility prediction in clinics. This approach offers a powerful platform in women's healthcare, not limited to OR and fertility.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Metabolomics and Mass Spectrometry Studies · Isotope Analysis in Ecology · Mass Spectrometry Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Shanghai Key Laboratory of Gynecologic Oncology Department of Obstetrics and Gynecology Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200127 P. R. China

State Key Laboratory of Systems Medicine for Cancer School of Biomedical Engineering and Institute of Medical Robotics Shanghai Jiao Tong University Shanghai 200030 P. R. China

Division of Cardiology Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200127 P. R. China

Department of Obstetrics and Gynecology the First Affiliated Hospital of Anhui Medical University Hefei 230022 P. R. China

NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract Anhui Medical University Hefei 230032 P. R. China

Key Laboratory of Population Health Across Life Cycle Anhui Medical University Hefei 230032 P. R. China

Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education) Renji Hospital Bio‐X Institutes School of Medicine Shanghai Jiao Tong University Shanghai 200240 P. R. China

Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education Ningxia Medical University Yinchuan 750003 P. R. China

Department of Obstetrics and Gynecology Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200127 P. R. China

Funding

National Natural Science Foundation of China · Science and Technology Commission of Shanghai Municipality · Shanghai Municipal Education Commission

References (56)

Show source-provided references
1. 10.1016/j.fertnstert.2020.09.13410.1016/j.fertnstert.2020.09.134 ↗
2. 10.1007/s10815-017-1058-410.1007/s10815-017-1058-4 ↗
3. 10.1007/s10815-021-02246-610.1007/s10815-021-02246-6 ↗
4. 10.1093/humrep/dey23810.1093/humrep/dey238 ↗
5. 10.1007/s10815-015-0595-y10.1007/s10815-015-0595-y ↗
6. 10.1016/j.ajog.2017.02.02710.1016/j.ajog.2017.02.027 ↗
7. 10.1002/VIW.2020015410.1002/VIW.20200154 ↗
8. 10.1002/smtd.20220026410.1002/smtd.202200264 ↗
9. 10.1002/VIW.2020010210.1002/VIW.20200102 ↗
10. 10.1002/smtd.20200100110.1002/smtd.202001001 ↗
11. 10.1038/s41467-020-17347-610.1038/s41467-020-17347-6 ↗
12. 10.1002/VIW.2020013810.1002/VIW.20200138 ↗
13. 10.1038/s41591-022-01980-310.1038/s41591-022-01980-3 ↗
14. 10.1001/jama.2017.1458810.1001/jama.2017.14588 ↗
15. 10.1186/s12958-021-00825-x10.1186/s12958-021-00825-x ↗
16. Zhang Y. · Front. Endocrinol. · 2020 · 11 · 3389
17. 10.1093/humrep/dex30310.1093/humrep/dex303 ↗
18. 10.3389/fcell.2021.77722410.3389/fcell.2021.777224 ↗
19. 10.1038/s41586-022-04828-510.1038/s41586-022-04828-5 ↗
20. 10.1002/VIW.2020006310.1002/VIW.20200063 ↗
21. 10.1002/smtd.20210122010.1002/smtd.202101220 ↗
22. 10.1002/adfm.20220667010.1002/adfm.202206670 ↗
23. 10.1038/s41587-020-0644-710.1038/s41587-020-0644-7 ↗
24. 10.1126/sciadv.abl492310.1126/sciadv.abl4923 ↗
25. 10.1021/acsanm.2c0185010.1021/acsanm.2c01850 ↗
26. 10.1002/mas.2167010.1002/mas.21670 ↗
27. 10.1002/VIW.2022004110.1002/VIW.20220041 ↗
28. 10.1073/pnas.212224511910.1073/pnas.2122245119 ↗
29. 10.1002/anie.20191306510.1002/anie.201913065 ↗
30. 10.1002/aisy.20210019110.1002/aisy.202100191 ↗
31. 10.1002/adma.20200797810.1002/adma.202007978 ↗
32. 10.1038/nprot.2007.37610.1038/nprot.2007.376 ↗
33. 10.1002/adma.20200090610.1002/adma.202000906 ↗
34. 10.1038/nprot.2011.33510.1038/nprot.2011.335 ↗
35. 10.1161/CIRCULATIONAHA.119.04165810.1161/CIRCULATIONAHA.119.041658 ↗
36. 10.1038/s42003-022-03710-410.1038/s42003-022-03710-4 ↗
37. 10.1016/j.rbmo.2018.10.00610.1016/j.rbmo.2018.10.006 ↗
38. 10.1016/j.fertnstert.2015.05.01710.1016/j.fertnstert.2015.05.017 ↗
39. 10.1016/j.fertnstert.2014.12.09310.1016/j.fertnstert.2014.12.093 ↗
40. 10.1093/humupd/dms04110.1093/humupd/dms041 ↗
41. 10.5653/cerm.2016.43.3.13910.5653/cerm.2016.43.3.139 ↗
42. 10.1080/01443615.2021.200330910.1080/01443615.2021.2003309 ↗
43. 10.1038/s41586-021-04278-510.1038/s41586-021-04278-5 ↗
44. 10.1038/s41420-021-00493-110.1038/s41420-021-00493-1 ↗
45. 10.1093/humupd/dmaa04310.1093/humupd/dmaa043 ↗
46. 10.1093/humrep/deac107.25410.1093/humrep/deac107.254 ↗
47. 10.1016/j.cell.2016.12.02610.1016/j.cell.2016.12.026 ↗
48. 10.1021/acsnano.1c0986410.1021/acsnano.1c09864 ↗
49. 10.1002/VIW.2020008210.1002/VIW.20200082 ↗
50. 10.1038/nbt.418310.1038/nbt.4183 ↗
51. 10.1186/s12958-021-00762-910.1186/s12958-021-00762-9 ↗
52. 10.1038/s41467-021-21121-710.1038/s41467-021-21121-7 ↗
53. 10.1002/jms.406810.1002/jms.4068 ↗
54. 10.1002/adma.20220142210.1002/adma.202201422 ↗
55. 10.1093/nar/gkab38210.1093/nar/gkab382 ↗
56. 10.1038/s41596-022-00710-w10.1038/s41596-022-00710-w ↗