journal article·2025-08Open access

RONIN/HCF1‐TFEB Axis Protects Against D‐Galactose‐Induced Cochlear Hair Cell Senescence Through Autophagy Activation

Yongjie Wei, Yuhua Zhang, Wei Cao, Nan Cheng, Yun Xiao, Yongjun Zhu, Yan Xu, Lei Zhang, Lingna Guo, Jun Song, Su‐hua Sha, Buwei Shao, Fang Ma, Jingwen Yang, Zheng Ying, Zuhong He, Renjie Chai, Qiaojun Fang, Jianming Yang

Advanced Science· Volume 12· Issue 29

Abstract

Abstract Age‐related hearing loss is characterized by senescent inner ear hair cells (HCs) and reduced autophagy. Despite the improved understanding of these processes, detailed molecular mechanisms underlying cochlear HC senescence remain unclear. Transcription Factor EB (TFEB), a key regulator of genes associated with autophagy and lysosomes, crucially affects aging‐related illnesses. However, intricate regulatory networks that influence TFEB activity remain to be thoroughly elucidated. The findings revealed that RONIN (THAP11), through its interaction with host cell factor C1 (HCF1/HCFC1), modulated the transcriptional activity of Tfeb , thus contributing to the mitigation (D‐galatactose [D‐gal]) senescent HC loss. Specifically, RONIN overexpression improved autophagy levels and lysosomal activity and attenuated changes associated with the senescence of HCs triggered by D‐gal. These findings highlight the possibility of using RONIN as a viable therapeutic target to ameliorate presbycusis by enhancing the TFEB function.
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Research context

OpenAlex topics: Antioxidants, Aging, Portulaca oleracea · Pediatric health and respiratory diseases · Hydrogen's biological and therapeutic effects

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Otolaryngology‐Head and Neck Surgery The Second Affiliated Hospital of Anhui Medical University Hefei 230601 China

Department of Pathology and Laboratory Medicine The Medical University of South Carolina Charleston SC 29425 USA

School of Medicine Faculty of Medical & Health Sciences Tel Aviv University Tel Aviv 6997801 Israel

Center for Scientific Research of Anhui Medical University Hefei 230032 China

International Department of Hefei 168 High School Hefei 230601 China

Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences Soochow University Suzhou 215123 China

Department of Otorhinolaryngology‐Head and Neck Surgery Zhongnan Hospital of Wuhan University Wuhan 430071 China

State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck Surgery Zhongda Hospital School of Life Sciences and Technology School of Medicine Advanced Institute for Life and Health Jiangsu Province High‐Tech Key Laboratory for Bio‐Medical Research Southeast University Nanjing 210096 China

Co‐Innovation Center of Neuroregeneration Nantong University Nantong 226001 China

Department of Neurology Aerospace Center Hospital School of Life Science Beijing Institute of Technology Beijing 100081 China

Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Chengdu 610072 China

Southeast University Shenzhen Research Institute Shenzhen 518063 China

Funding

National Natural Science Foundation of China · National Key Research and Development Program of China · Natural Science Foundation of Jiangsu Province · Science and Technology Department of Sichuan Province

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