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journal article·2024-06Open access

Proteomic Identification of Small Extracellular Vesicle Proteins LAMB1 and Histone H4 for Prostate Cancer Diagnosis and Risk Stratification

Bairen Pang, Qi Wang, Haotian Chen, Zhihan Liu, Meng Han, Jie Gong, Liang Yue, Xuan Ding, Suying Wang, Zejun Yan, Yingzhi Chen, David Malouf, Joseph Bucci, Tiannan Guo, Cheng Zhou, Junhui Jiang, Yong Li

Advanced Science· Volume 11· Issue 23
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Source abstract

Abstract

Abstract Diagnosis and stratification of prostate cancer (PCa) patients using the prostate‐specific antigen (PSA) test is challenging. Extracellular vesicles (EVs), as a new star of liquid biopsy, has attracted interest to complement inaccurate PSA screening and invasiveness of tissue biopsy. In this study, a panel of potential small EV (sEV) protein biomarkers is identified from PCa cell lines using label‐free LC‐MS/MS proteomics. These biomarkers underwent further validation with plasma and urine samples from different PCa stages through parallel reaction monitoring‐based targeted proteomics, western blotting, and ELISA. Additionally, a tissue microarray containing cancerous and noncancerous tissues is screened to provide additional evidence of selected sEV proteins associated with cancer origin. Results indicate that sEV protein LAMB1 is highly expressed in human plasma of metastatic PCa patients compared with localised PCa patients and control subjects, while sEV protein Histone H4 is highly expressed in human urine of high‐risk PCa patients compared to low‐risk PCa patients and control subjects. These two sEV proteins demonstrate higher specificity and sensitivity than the PSA test and show promise for metastatic PCa diagnosis, progression monitoring, and risk stratification.
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Research context

OpenAlex topics: Extracellular vesicles in disease · Prostate Cancer Treatment and Research · Inflammatory Biomarkers in Disease Prognosis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Urology The First Affiliated Hospital of Ningbo University Ningbo Zhejiang 315010 China

Ningbo Clinical Research Centre for Urological Disease The First Affiliated Hospital of Ningbo University Ningbo Zhejiang 315010 China

Translational Research Laboratory for Urology The Key Laboratory of Ningbo The First Affiliated Hospital of Ningbo University Ningbo Zhejiang 315010 China

Zhejiang Engineering Research Center of Innovative technologies and diagnostic and therapeutic equipment for urinary system diseases Ningbo Zhejiang 315010 China

Cancer Care Centre St George Hospital Kogarah NSW 2217 Australia

St. George and Sutherland Clinical Campuses School of Clinical Medicine UNSW Sydney Kensington NSW 2052 Australia

Health Science Centre Ningbo University Ningbo Zhejiang 315211 China

Westlake Centre for Intelligent Proteomics Westlake Laboratory of Life Sciences and Biomedicine Hangzhou Zhejiang 310030 China

Key Laboratory of Structural Biology of Zhejiang Province School of Life Sciences Westlake University Hangzhou Zhejiang 310030 China

Department of Pathology Ningbo Diagnostic Pathology Centre Ningbo Zhejiang 315021 China

Department of Urology St George Hospital Kogarah NSW 2217 Australia

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