DataVXAdvanced MaterialsPaper
journal article·2023-09Open access

Reduced Graphene Oxide Electrolyte‐Gated Transistor Immunosensor with Highly Selective Multiparametric Detection of Anti‐Drug Antibodies

Matteo Sensi, Rafael Furlan de Oliveira, Marcello Berto, Marina Palmieri, Emilio Ruini, Pietro Antonio Livio, Andrea Conti, Marcello Pinti, Carlo Salvarani, Andrea Cossarizza, Joan M. Cabot, Jordi Ricart, Stefano Casalini, María Begoña González‐García, Pablo Fanjul‐Bolado, Carlo Augusto Bortolotti, Paolo Samorì, Fabio Biscarini

Advanced Materials· Volume 35· Issue 36
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Source abstract

Abstract

Abstract The advent of immunotherapies with biological drugs has revolutionized the treatment of cancers and auto‐immune diseases. However, in some patients, the production of anti‐drug antibodies (ADAs) hampers the drug efficacy. The concentration of ADAs is typically in the range of 1–10 pm; hence their immunodetection is challenging. ADAs toward Infliximab (IFX), a drug used to treat rheumatoid arthritis and other auto‐immune diseases, are focussed. An ambipolar electrolyte‐gated transistor (EGT) immunosensor is reported based on a reduced graphene oxide (rGO) channel and IFX bound to the gate electrode as the specific probe. The rGO‐EGTs are easy to fabricate and exhibit low voltage operations (≤ 0.3 V), a robust response within 15 min, and ultra‐high sensitivity (10 am limit of detection). A multiparametric analysis of the whole rGO‐EGT transfer curves based on the type‐I generalized extreme value distribution is proposed. It is demonstrated that it allows to selectively quantify ADAs also in the co‐presence of its antagonist tumor necrosis factor alpha (TNF‐α), the natural circulating target of IFX.
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Research context

Energy storageNanomaterialsElectronic materials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Graphene and Nanomaterials Applications · Graphene research and applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Life Sciences University of Modena and Reggio Emilia via Campi 103 Modena 41125 Italy

Université de Strasbourg, CNRS, ISIS 8 allée Gaspard Monge Strasbourg 67000 France

Brazilian Nanotechnology National Laboratory (LNNano) Brazilian Center for Research in Energy and Materials (CNPEM) Campinas 13083‐970 Brazil

Dermatology Unit Surgical Medical and Dental Department of Morphological Sciences Related to Transplant Oncology and Regenerative Medicine University of Modena and Reggio Emilia via del Pozzo 71 Modena 41125 Italy

Rheumatology Unit University of Modena and Reggio Emilia Medical School Azienda Ospedaliero‐Universitaria Policlinico di Modena via del Pozzo 71 Modena 41125 Italy

Department of Medical and Surgical Sciences for Children and Adults University of Modena and Reggio Emilia Via Campi 287 Modena 41125 Italy

Leitat Technology Center Innovació 2 Barcelona 08225 Spain

Dipartimento di Scienze Chimiche University of Padova via Marzolo 1 Padova 35131 Italy

Metrohm DropSens S.L. Vivero Ciencias de la Salud C/Colegio Santo Domingo de Guzmán s/n Oviedo 33010 Spain

Center for Translational Neurophysiology Istituto Italiano di Tecnologia Via Fossato di Mortara 17–19 Ferrara 44121 Italy

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