DataVXAdvanced MaterialsPaper
journal article·2018-08Open access

DNA Nanotweezers and Graphene Transistor Enable Label‐Free Genotyping

Michael T. Hwang, Zejun Wang, Jinglei Ping, Deependra Kumar Ban, Zi Chao Shiah, Leif Antonschmidt, Joon Lee, Yushuang Liu, Abhijith G. Karkisaval, Alan T. Charlie Johnson, Chunhai Fan, Gennadi Glinsky, Ratnesh Lal

Advanced Materials· Volume 30· Issue 34
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Source abstract

Abstract

Abstract Electronic DNA‐biosensor with a single nucleotide resolution capability is highly desirable for personalized medicine. However, existing DNA‐biosensors, especially single nucleotide polymorphism (SNP) detection systems, have poor sensitivity and specificity and lack real‐time wireless data transmission. DNA‐tweezers with graphene field effect transistor (FET) are used for SNP detection and data are transmitted wirelessly for analysis. Picomolar sensitivity of quantitative SNP detection is achieved by observing changes in Dirac point shift and resistance change. The use of DNA‐tweezers probe with high‐quality graphene FET significantly improves analytical characteristics of SNP detection by enhancing the sensitivity more than 1000‐fold in comparison to previous work. The electrical signal resulting from resistance changes triggered by DNA strand‐displacement and related changes in the DNA geometry is recorded and transmitted remotely to personal electronics. Practical implementation of this enabling technology will provide cheaper, faster, and portable point‐of‐care molecular health status monitoring and diagnostic devices.
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Research context

NanomaterialsElectronic materials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Molecular Junctions and Nanostructures · Nanowire Synthesis and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Materials Science and Engineering Program University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA

Division of Physical Biology and Bioimaging Center Shanghai Synchrotron Radiation Facility CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Shanghai 201800 China

Department of Physics and Astronomy University of Pennsylvania Philadelphia PA 19104 USA

Department of Mechanical and Aerospace Engineering University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA

Department of Electrical and Computer Engineering University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA

NMR Based Structural Biology Max Planck Institute for Biophysical Chemistry Am Fassberg 11 37077 Göttingen Germany

School of Life Science Inner Mongolia Agricultural University 306 Zhaowuda Road Hohhot 010018 China

Institute of Engineering in Medicine University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA

Department of Bioengineering University of California San Diego 9500 Gilman Drive La Jolla CA 92093 USA

Funding

National Institute on Drug Abuse · National Institute on Aging · National Institute of Environmental Health Sciences

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