DataVXAdvanced Healthcare MaterialsPaper
journal article·2023-08Open access

A Non‐G‐Quadruplex DNA Aptamer Targeting NCL for Diagnosis and Therapy in Bladder Cancer

Yunyi Liu, Bei Hu, Xiaming Pei, Juan Li, Dan Qi, Yuxi Xu, Hailong Ou, Yatao Wu, Lei Xue, Jason H. Huang, Erxi Wu, Xiaoxiao Hu

Advanced Healthcare Materials· Volume 12· Issue 20
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Abstract

Abstract Bladder cancer (BC) is a highly aggressive malignant tumor affecting the urinary system, characterized by metastasis and a poor prognosis that often leads to limited therapeutic success. This study aims to develop a novel DNA aptamer for the diagnosis and treatment of BC using a tissue‐based systematic evolution of ligands by an exponential enrichment (SELEX) process. By using SELEX, this work successfully generates a new aptamer named TB‐5, which demonstrates a remarkable and specific affinity for nucleolin (NCL) in BC tissues and displays marked biocompatibility both in vitro and in vivo. Additionally, this work shows that NCL is a reliable tissue‐specific biomarker in BC. Moreover, according to circular dichroism spectroscopy, TB‐5 forms a non‐G‐quadruplex structure, distinguishing it from the current NCL–targeting aptamer AS1411, and exhibits a distinct binding region on NCL compared to AS1411. Notably, this study further reveals that TB‐5 activates NCL function by promoting autophagy and suppressing the migration and invasion of BC cells, which occurs by disrupting mRNA transcription processes. These findings highlight the critical role of NCL in the pathological examination of BC and warrant more comprehensive investigations on anti‐NCL aptamers in BC imaging and treatment.
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Research context

Biomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · RNA modifications and cancer

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology Molecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan Province Hunan University Changsha Hunan 410082 China

Department of Urology Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine. Changsha Hunan 410013 China

Department of Neurosurgery and Neuroscience Institute Baylor Scott & White Health Temple TX 76508 USA

Department of Pathology Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine. Changsha Hunan 410013 China

Department of Medical Education Texas A&M University School of Medicine College Station TX 77843 USA

Department of Pharmaceutical Sciences Texas A&M University School of Pharmacy College Station TX 77843 USA

LIVESTRONG Cancer Institutes and Department of Oncology Dell Medical School The University of Texas at Austin Austin TX 78712 USA

Research Institute of Hunan University in Chongqing Chongqing 401120 China

Shenzhen Research Institute Hunan University Shenzhen Guangdong 518000 China

Hunan Yonghe‐sun Biotechnology Co. Ltd. Changsha Hunan 410082 China

Funding

National Natural Science Foundation of China · Natural Science Foundation of Chongqing

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