Enhanced antimicrobial efficacy of a vancomycin/zinc oxide/chitosan nanocomposite via Bacillus licheniformis-mediated biomodification
Source abstract
Abstract
Abstract Recognition of antimicrobial resistance (AMR) is crucial for a strong publication. Drug-resistant microbes, such as Candida albicans , methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli , Proteus mirabilis , and Klebsiella pneumoniae , pose a significant health threat. There is an urgent need for innovative and synergistic therapies. The new engineered nanocomposite system, zinc oxide/chitosan nanocomposite loaded with vancomycin (VA/ZnO/CS), directly addresses this challenge by aiming to enhance or restore the efficacy of existing drugs. Zinc oxide nanoparticles (ZnO NPs) were biosynthesized using Bacillus licheniformis ATCC 4527, and then combined with chitosan (CS) and vancomycin (VA) through a green chemical method. The nanocomposite that was produced was characterized using various techniques. The results of UV–Vis spectroscopy showed an adsorption peak at 348 nm. The material matrix of the nanocomposite contains ZnO NPs and numerous active groups, as indicated by the results of X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). Images captured by transmission electron microscopy (TEM) showed that the VA/ZnO/CS particles were spherical with an average size of 78 ± 2.3 nm. The mean crystallite size of the nanocomposite was calculated using the Scherrer equation from the XRD data (79.38 nm) which closely matched the dimensions of the ZnO core observed in the TEM images (78 ± 2.3 nm). The antimicrobial activity of VA/ZnO/CS was tested against Bacillus cereus ATCC 14,579, MRSA ATCC 33,592, P. mirabilis AUF1, Klebsiella pneumoniae ATCC 11,296, and Candida albicans ATCC 10,231. Compared to common drugs like fluconazole and vancomycin, VA/ZnO/CS demonstrated significantly higher levels of biocidal activity in the agar well-diffusion test, minimum inhibitory concentration (MIC), and minimum microbicidal concentration (MMC). The antimicrobial activity was found to be dependent on the dose of nanocomposite with higher doses resulting in increased antimicrobial inhibition. The prepared nanocomposite achieved a complete biocidal effect against the investigated microorganisms with 5–15 µg/ml, while conventional drugs required 25–30 µg/ml. The powerful antimicrobial action of VA/ZnO/CS was demonstrated by the TEM micrographs of C. albicans showing malformations and distortions of cell structure, including cell wall destruction and the emergence of vacuoles. Based on the results, the green synergy between ZnO/CS nanocomposite and VA will provide an effective biomaterial for treating infections and microbial diseases.
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Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Antimicrobial agents and applications · Bacterial biofilms and quorum sensing
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (100+)
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1. James R, Hardefeldt LY, Ierano C, Charani E, Dowson L, Elkins S, et al. Antimicrobial stewardship from a one health perspective. Nat Rev Microbiol. 2025. https://doi.org/10.1038/s41579-025-01233-3.10.1038/s41579-025-01233-3 ↗
2. Tang KWK, Millar BC, Moore JE. Antimicrobial resistance (AMR). Br J Biomed Sci. 2023;80:11387. https://doi.org/10.3389/bjbs.2023.11387.10.3389/bjbs.2023.11387 ↗
3. Aslam B, Asghar R, Muzammil S, Shafique M, Siddique AB, Khurshid M, et al. AMR and sustainable development goals: at a crossroads. Glob Health. 2024;20(1):73. https://doi.org/10.1186/s12992-024-01046-8.10.1186/s12992-024-01046-8 ↗
4. Nazir A, Nazir A, Zuhair V, Aman S, Sadiq SUR, Hasan AH, et al. The global challenge of antimicrobial resistance: mechanisms, case studies, and mitigation approaches. Health Sci Rep. 2025;8(7):e71077. https://doi.org/10.1002/hsr2.71077.10.1002/hsr2.71077 ↗
5. Poulain D. Candida albicans, plasticity and pathogenesis. Crit Rev Microbiol. 2015;41(2):208–17. https://doi.org/10.3109/1040841X.2013.813904.10.3109/1040841X.2013.813904 ↗
6. Logan C, Martin-Loeches I, Bicanic T. Invasive candidiasis in critical care: challenges and future directions. Intensive Care Med. 2020;46(11):2001–14. https://doi.org/10.1007/s00134-020-06240-x.10.1007/s00134-020-06240-x ↗
7. Branda F, Petrosillo N, Ceccarelli G, Giovanetti M, De Vito A, Madeddu G, et al. Antifungal agents in the 21st century: advances, challenges, and future perspectives. Infect Dis Rep. 2025;17(4):91. https://doi.org/10.3390/idr17040091.10.3390/idr17040091 ↗
8. Sinha S, Upadhyay LSB. Understanding antimicrobial resistance (AMR) mechanisms and advancements in AMR diagnostics. Diagn Microbiol Infect Dis. 2025;113(2):116949. https://doi.org/10.1016/j.diagmicrobio.2025.116949.10.1016/j.diagmicrobio.2025.116949 ↗
9. Elshobary ME, Badawy NK, Ashraf Y, Zatioun AA, Masriya HH, Ammar MM, et al. Combating antibiotic resistance: mechanisms, multidrug-resistant pathogens, and novel therapeutic approaches: an updated review. Pharmaceuticals. 2025;18(3):402. https://doi.org/10.3390/ph18030402.10.3390/ph18030402 ↗
10. Altarac D, Gutch M, Mueller J, Ronsheim M, Tommasi R, Perros M. Challenges and opportunities in the discovery, development, and commercialization of pathogen-targeted antibiotics. Drug Discov Today. 2021;26(9):2084–9. https://doi.org/10.1016/j.drudis.2021.02.014.10.1016/j.drudis.2021.02.014 ↗
11. Theuretzbacher U. The global resistance problem and the clinical antibacterial pipeline. Nat Rev Microbiol. 2025;23(8):491–508. https://doi.org/10.1038/s41579-025-01169-8.10.1038/s41579-025-01169-8 ↗
12. Bothe H, Chhajed S, Bothe S. Antimicrobial resistance and its societal implications: a comprehensive review. Int J Pathog Res. 2024;13(6):202–18. https://doi.org/10.9734/ijpr/2024/v13i6334.10.9734/ijpr/2024/v13i6334 ↗
13. Bharti S, Kumar A. Nanotechnology in targeted delivery of antimicrobials and overcoming resistance. Bionanoscience. 2024;15(1):20. https://doi.org/10.1007/s12668-024-01654-z.10.1007/s12668-024-01654-z ↗
14. Elshikiby L, Baka Z, El-Zahed M. Antimicrobial activity of biosynthesized selenium nanoparticles using Pseudomonas aeruginosa isolate. Sci J Damietta Fac Sci. 2025;15(2):1. https://doi.org/10.21608/sjdfs.2025.387130.1231.10.21608/sjdfs.2025.387130.1231 ↗
15. Baka Z, Elshikiby L, El-Zahed M. Synthesis, characterization and biological applications of green synthesized se NPs using locally isolated multidrug-resistant Escherichia coli. Sci J Damietta Fac Sci. 2025;15(2):1–13. https://doi.org/10.21608/sjdfs.2025.387519.1232.10.21608/sjdfs.2025.387519.1232 ↗
16. Elshikiby LA, Baka ZAM, El-Zahed MM. Biological activities of optimized biosynthesized selenium nanoparticles using Proteus mirabilis PQ350419 alone or combined with chitosan and ampicillin against common multidrug-resistant bacteria. Microb Cell Fact. 2025;24(1):159. https://doi.org/10.1186/s12934-025-02783-0.10.1186/s12934-025-02783-0 ↗
17. El-Fawal MF, El-Fallal AA, Abou-Dobara MI, El-Sayed AKA, El-Zahed MM. In vitro antibacterial activity of myco-synthesized selenium nanoparticles against a multidrug-resistant E. coli isolated from clinical specimens. Discov Mater. 2025;5(1):130. https://doi.org/10.1007/s43939-025-00330-7.10.1007/s43939-025-00330-7 ↗
18. Salman MF, Al-Mudallal NHAL, Ahmed ME. Cytotoxic effect of biogenic selenium nanoparticles using bacteriocin of Acinetobacter baumannii isolated from burns and wound infections. Iraq J Sci. 2025;66(4):1535–49. https://doi.org/10.24996/ijs.2025.66.4.13.10.24996/ijs.2025.66.4.13 ↗
19. Ahmed ME, Hamza Faiq N, Almutairi HH, Alam MW. Biosynthesized ZnO–CuO nanocomposite for biofilm formation of Proteus mirabilis upon LuxS gene expression. Inorganics (Basel). 2025;13(2):65. https://doi.org/10.3390/inorganics13020065.10.3390/inorganics13020065 ↗
20. Ahmed ME, Alzahrani KK, Fahmy NM, Almutairi HH, Almansour ZH, Alam MW. Colistin-conjugated selenium nanoparticles: a dual-action strategy against drug-resistant infections and cancer. Pharmaceutics. 2025;17(5):556. https://doi.org/10.3390/pharmaceutics17050556.10.3390/pharmaceutics17050556 ↗
21. Majumder DD, Banerjee R, Ulrichs CH, Mewis I, Goswami A. Nano-materials: science of bottom-up and top-down. IETE Tech Rev. 2007;24(1):9–25. https://doi.org/10.4103/02564602.10876577.10.4103/02564602.10876577 ↗
22. Guo X, Zhang Q, Ding X, Shen Q, Wu C, Zhang L, et al. Synthesis and application of several sol–gel-derived materials via sol–gel process combining with other technologies: a review. J Solgel Sci Technol. 2016;79(2):328–58. https://doi.org/10.1007/s10971-015-3935-6.10.1007/s10971-015-3935-6 ↗
23. Salem SS, Fouda A. Green synthesis of metallic nanoparticles and their prospective biotechnological applications: an overview. Biol Trace Elem Res. 2021;199(1):344–70. https://doi.org/10.1007/s12011-020-02138-3.10.1007/s12011-020-02138-3 ↗
24. El-Zahed AAZ, Khalifa ME, El-Zahed MM, Baka ZA. Biological synthesis and characterization of antibacterial manganese oxide nanoparticles using Bacillus subtilis ATCC6633. Sci J Damietta Fac Sci. 2023;13(3):79–87. https://doi.org/10.21608/sjdfs.2023.242279.1136.10.21608/sjdfs.2023.242279.1136 ↗
25. Baka ZA, El-Zahed MM. Antifungal activity of silver/silicon dioxide nanocomposite on the response of faba bean plants (Vicia faba L.) infected by botrytis cinerea. Bioresour Bioprocess. 2022;9(1):102. https://doi.org/10.1186/s40643-022-00591-7.10.1186/s40643-022-00591-7 ↗
26. Baka ZAM, El-Zahed MM. Phenolic acids and defense-related enzymes in host-pathogen interaction in Senecio aegyptius and the rust fungus, Puccinia lagenophorae. Arch Phytopathol Plant Prot. 2023;56:721–752.10.1080/03235408.2023.2216368 ↗
27. Fayed RM, Mohamed Baka ZA, Farouk BH, El-Zahed MM. Antibacterial and cytotoxic activities of a newly green synthesized ZnO/Se nanocomposite combined with Washingtonia robusta H. Wendl fruit extract. Biocatal Agric Biotechnol. 2025;64:103500. https://doi.org/10.1016/j.bcab.2025.103500.10.1016/j.bcab.2025.103500 ↗
28. Mohd Yusof H, Rahman A, Mohamad R, Zaidan UH, Samsudin AA. Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties. Sci Rep. 2020;10(1):1–13. https://doi.org/10.1038/s41598-020-76402-w.10.1038/s41598-020-76402-w ↗
29. Alam H, Khatoon N, Khan MA, Husain SA, Saravanan M, Sardar M. Synthesis of selenium nanoparticles using probiotic bacteria Lactobacillus acidophilus and their enhanced antimicrobial activity against resistant bacteria. J Clust Sci. 2020;31(5):1003–11. https://doi.org/10.1007/S10876-019-01705-6.10.1007/S10876-019-01705-6 ↗
30. Mohamed EA, El-Zahed MM. Anticandidal applications of selenium nanoparticles biosynthesized with Limosilactobacillus fermentum (OR553490). Discov Nano. 2024;19(1):115. https://doi.org/10.1186/s11671-024-04055-z.10.1186/s11671-024-04055-z ↗
31. Muras A, Romero M, Mayer C, Otero A. Biotechnological applications of Bacillus licheniformis. Crit Rev Biotechnol. 2021;41(4):609–27. https://doi.org/10.1080/07388551.2021.1873239.10.1080/07388551.2021.1873239 ↗
32. Ramirez-Olea H, Reyes-Ballesteros B, Chavez-Santoscoy RA. Potential application of the probiotic Bacillus licheniformis as an adjuvant in the treatment of diseases in humans and animals: a systematic review. Front Microbiol. 2022;13:993451. https://doi.org/10.3389/fmicb.2022.993451.10.3389/fmicb.2022.993451 ↗
33. Abinaya M, Gnanaprakasam P, Govindarajan M, Wadaan MA, Mahboob S, Wadaan AM, et al. Antibacterial and antibiofilm potential of microbial polysaccharide overlaid zinc oxide nanoparticles and selenium nanowire. Fermentation. 2022;8(11):637. https://doi.org/10.3390/fermentation8110637.10.3390/fermentation8110637 ↗
34. Hassan ME, Hassan RR, Diab KA, El-Nekeety AA, Hassan NS, Abdel-Wahhab MA. Nanoencapsulation of thyme essential oil: a new avenue to enhance its protective role against oxidative stress and cytotoxicity of zinc oxide nanoparticles in rats. Environ Sci Pollut Res. 2021;28(37):52046–63. https://doi.org/10.1007/s11356-021-14427-y.10.1007/s11356-021-14427-y ↗
35. Zhang R, Carlsson F, Edman M, Hummelgård M, Jonsson B-G, Bylund D, et al. Escherichia coli bacteria develop adaptive resistance to antibacterial ZnO nanoparticles. Adv Biosyst. 2018;2(5):1800019. https://doi.org/10.1002/adbi.201800019.10.1002/adbi.201800019 ↗
36. Wang X, Yang F, Zhao J, Xu Y, Mao D, Zhu X, et al. Bacterial exposure to ZnO nanoparticles facilitates horizontal transfer of antibiotic resistance genes. NanoImpact. 2018;10:61–7. https://doi.org/10.1016/j.impact.2017.11.006.10.1016/j.impact.2017.11.006 ↗
37. Gao Y, Arokia Vijaya Anand M, Ramachandran V, Karthikkumar V, Shalini V, Vijayalakshmi S, et al. Biofabrication of zinc oxide nanoparticles from Aspergillus niger, their antioxidant, antimicrobial and anticancer activity. J Clust Sci. 2019;30(4):937–46. https://doi.org/10.1007/s10876-019-01551-6.10.1007/s10876-019-01551-6 ↗
38. Mohd Yusof H, Abdul Rahman N, Mohamad R, Zaidan UH, Samsudin AA. Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties. Sci Rep. 2020;10(1):19996. https://doi.org/10.1038/s41598-020-76402-w.10.1038/s41598-020-76402-w ↗
39. Shahverdi AR, Minaeian S, Shahverdi HR, Jamalifar H, Nohi A-A. Rapid synthesis of silver nanoparticles using culture supernatants of enterobacteria: a novel biological approach. Process Biochem. 2007;42(5):919–23. https://doi.org/10.1016/j.procbio.2007.02.005.10.1016/j.procbio.2007.02.005 ↗
40. Al-Naamani L, Dobretsov S, Dutta J, Burgess JG. Chitosan-zinc oxide nanocomposite coatings for the prevention of marine biofouling. Chemosphere. 2017;168:408–17. https://doi.org/10.1016/j.chemosphere.2016.10.033.10.1016/j.chemosphere.2016.10.033 ↗
41. Clinical and Laboratory Standards. Performance standards for antimicrobial susceptibility testing. Performance standards for antimicrobial susceptibility testing: approved standard- twenty-seven edition, clinical and laboratory standards Institute. Wayne, PA: Clin Lab Stand Inst; 2017.
42. CLSI. Performance standards for antimicrobial susceptibility testing: approved standard-twenty-seven edition. Wayne: Clin Lab Stand Inst; 2017.
43. Clinical and Laboratory Standards. Reference method for broth Dilution antifungal susceptibility testing of yeasts; approved standard M27-A3. Wayne, PA: Clin Lab Stand Inst; 2008.
44. Clinical and Laboratory Standards. Methods for Dilution antimicrobial susceptibility test for bacteria that grow aerobically. Wayne, PA: Clin Lab Stand Inst; 2000.
45. Espinel-Ingroff A, Fothergill A, Peter J, Rinaldi MG, Walsh TJ. Testing conditions for determination of minimum fungicidal concentrations of new and established antifungal agents for Aspergillus spp.: NCCLS collaborative study. J Clin Microbiol. 2002;40(9):3204–8.10.1128/JCM.40.9.3204-3208.2002 ↗
46. O’connor BP. SPSS and SAS programs for determining the number of components using parallel analysis and velicer’s MAP test. Behav Res Methods Instrum Comput. 2000;32:396–402.10.3758/BF03200807 ↗
47. Singh R, Shedbalkar UU, Wadhwani SA, Chopade BA. Bacteriagenic silver nanoparticles: synthesis, mechanism, and applications. Appl Microbiol Biotechnol. 2015;99(11):4579–93. https://doi.org/10.1007/s00253-015-6622-1.10.1007/s00253-015-6622-1 ↗
48. Yusof HM, Mohamad R, Zaidan UH. Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4. Microb Cell Fact. 2020;19(1):1–17.10.1186/s12934-019-1269-8 ↗
49. Markus J, Mathiyalagan R, Kim Y-J, Abbai R, Singh P, Ahn S, et al. Intracellular synthesis of gold nanoparticles with antioxidant activity by probiotic Lactobacillus kimchicus DCY51T isolated from Korean Kimchi. Enzyme Microb Technol. 2016;95:85–93. https://doi.org/10.1016/j.enzmictec.2016.08.018.10.1016/j.enzmictec.2016.08.018 ↗
50. Ali AA, Asif MA, Mashrai AM, Khanam HK. Green synthesis of ZnO nanoparticles using Bacillus subtilis and their catalytic performance in the one-pot synthesis of steroidal thiophenes. Eur Chem Bull. 2014;3(9):939–45.
51. Jayaseelan C, Rahuman AA, Kirthi AV, Marimuthu S, Santhoshkumar T, Bagavan A, et al. Novel microbial route to synthesize ZnO nanoparticles using Aeromonas hydrophila and their activity against pathogenic bacteria and fungi. Spectrochim Acta Mol Biomol Spectrosc. 2012;90:78–84. https://doi.org/10.1016/j.saa.2012.01.006.10.1016/j.saa.2012.01.006 ↗
52. Salman JAS, Kadhim AA, Haider AJ. Biosynthesis, characterization and antibacterial effect of ZnO nanoparticles synthesized by Lactobacillus spp. J Glob Pharma Technol. 2018;10(03):348–55.
53. Stroyuk OL, Dzhagan VM, Shvalagin VV, Kuchmiy SY. Size-dependent optical properties of colloidal ZnO nanoparticles charged by photoexcitation. J Phys Chem C. 2010;114(1):220–5. https://doi.org/10.1021/jp908879h.10.1021/jp908879h ↗
54. Chen M, Straatsma TP, Fang Z, Dixon DA. Structural and electronic property study of ZnOn, 168: transition from zinc oxide molecular clusters to ultrasmall nanoparticles. J Phys Chem C. 2016;120(36):20400–18. https://doi.org/10.1021/acs.jpcc.6b06730.10.1021/acs.jpcc.6b06730 ↗
55. Dutta RK, Nenavathu BP, Gangishetty MK, Reddy AVR. Studies on antibacterial activity of ZnO nanoparticles by ROS induced lipid peroxidation. Colloids Surf B Biointerfaces. 2012;94:143–50. https://doi.org/10.1016/j.colsurfb.2012.01.046.10.1016/j.colsurfb.2012.01.046 ↗
56. Al-Harbi LM, Alsulami QA, Farea MO, Rajeh A. Tuning optical, dielectric, and electrical properties of polyethylene oxide/carboxymethyl cellulose doped with mixed metal oxide nanoparticles for flexible electronic devices. J Mol Struct. 2023;1272:134244. https://doi.org/10.1016/j.molstruc.2022.134244.10.1016/j.molstruc.2022.134244 ↗
57. Kołodziejczak-Radzimska A, Jesionowski T. Zinc oxide—from synthesis to application: a review. Materials. 2014;7(4):2833–81. https://doi.org/10.3390/ma7042833.10.3390/ma7042833 ↗
58. Rajabairavi N, Raju CS, Karthikeyan C, Varutharaju K, Nethaji S, Hameed ASH, et al. Biosynthesis of novel zinc oxide nanoparticles (ZnO NPs) using endophytic bacteria Sphingobacterium thalpophilum. In: Recent trends in materials science and applications. Springer; 2017. pp. 245–54. https://doi.org/10.1007/978-3-319-44890-9_23.10.1007/978-3-319-44890-9_23 ↗
59. Abd El-Nour AT, Abou-Dobara MI, El-Sayed AKA, El-Zahed MM. Extracellular biosynthesis and antimicrobial activity of Bacillus subtilis ATCC 6633 zinc oxide nanoparticles. Sci J Damietta Fac Sci. 2023;13(2):39–47. https://doi.org/10.21608/sjdfs.2023.173178.1064.10.21608/sjdfs.2023.173178.1064 ↗
60. EL-Ghwas DE. Characterization and biological synthesis of zinc oxide nanoparticles by new strain of Bacillus foraminis. Biodiversitas. 2022;23(1). https://doi.org/10.13057/biodiv/d230159.10.13057/biodiv/d230159 ↗
61. Abd El-Nour AT, Abou-Dobara MI, El-Sayed AKA, El-Zahed MM. Antibacterial activity of optimized extracellular biosynthesized zinc oxide nanoparticles using Corynebacterium sp. ATCC 6931. Sci J Damietta Fac Sci. 2023;13(3):63–70. https://doi.org/10.21608/sjdfs.2023.231788.1129.10.21608/sjdfs.2023.231788.1129 ↗
62. Shanmugam R, Munusamy T, Jayakodi S, Al-Ghanim KA, Nicoletti M, Sachivkina N, et al. Probiotic-bacteria (Lactobacillus fermentum)-wrapped zinc oxide nanoparticles: biosynthesis, characterization, and antibacterial activity. Fermentation. 2023;9(5):413. https://doi.org/10.3390/fermentation9050413.10.3390/fermentation9050413 ↗
63. Phogat P, Shreya, Jha R, Singh S. Harnessing ZnO morphologies in energy application and sustainable development. Phys Scr. 2024;99(10):102004. https://doi.org/10.1088/1402-4896/ad7990.10.1088/1402-4896/ad7990 ↗
64. Wong-Ng W, McMurdie HF, Hubbard CR, Mighell AD. JCPDS-ICDD research associateship (cooperative program with NBS/NIST). J Res Natl Inst Stand Technol. 2001;106(6):1013–28. https://doi.org/10.6028/jres.106.052.10.6028/jres.106.052 ↗
65. Harish Prashanth K. Solid state structure of chitosan prepared under different N-deacetylating conditions. Carbohydr Polym. 2002;50(1):27–33. https://doi.org/10.1016/S0144-8617(01)00371-X.10.1016/S0144-8617(01)00371-X ↗
66. Priyadarshi R, Kim S-M, Rhim J-W. Carboxymethyl cellulose-based multifunctional film combined with zinc oxide nanoparticles and grape seed extract for the preservation of high-fat meat products. Sustain Mater Technol. 2021;29:e00325. https://doi.org/10.1016/j.susmat.2021.e00325.10.1016/j.susmat.2021.e00325 ↗
67. Abd-Elraoof WA, Tayel AA, Shaymaa W, Abukhatwah OMW, Diab AM, Abonama OM, et al. Characterization and antimicrobial activity of a chitosan-selenium nanocomposite biosynthesized using Posidonia oceanica. RSC Adv. 2023;13(37):26001–14.10.1039/D3RA04288J ↗
68. Mahdi ZS, Talebnia Roshan F, Nikzad M, Ezoji H. Biosynthesis of zinc oxide nanoparticles using bacteria: a study on the characterization and application for electrochemical determination of bisphenol A. Inorg Nano-Metal Chem. 2021;51(9):1249–57. https://doi.org/10.1080/24701556.2020.1835962.10.1080/24701556.2020.1835962 ↗
69. Djearamane S, Xiu L-J, Wong L-S, Rajamani R, Bharathi D, Kayarohanam S, et al. Antifungal properties of zinc oxide nanoparticles on Candida albicans. Coatings. 2022;12(12):1864. https://doi.org/10.3390/coatings12121864.10.3390/coatings12121864 ↗
70. Sirelkhatim A, Mahmud S, Seeni A, Kaus NHM, Ann LC, Bakhori SKM, et al. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nanomicro Lett. 2015;7(3):219–42. https://doi.org/10.1007/s40820-015-0040-x.10.1007/s40820-015-0040-x ↗
71. Gondal MA, Drmosh QA, Yamani ZH, Saleh TA. Synthesis of ZnO nanoparticles by laser ablation in liquid and their annealing transformation into ZnO nanoparticles. Appl Surf Sci. 2009;256(1):298–304. https://doi.org/10.1016/j.apsusc.2009.08.019.10.1016/j.apsusc.2009.08.019 ↗
72. Mehta M, Allen-Gipson D, Mohapatra S, Kindy M, Limayem A. Study on the therapeutic index and synergistic effect of chitosan-zinc oxide nanomicellar composites for drug-resistant bacterial biofilm Inhibition. Int J Pharm. 2019;565:472–80. https://doi.org/10.1016/j.ijpharm.2019.05.003.10.1016/j.ijpharm.2019.05.003 ↗
73. Rehman S, Jermy BR, Akhtar S, Borgio JF, Abdul Azeez S, Ravinayagam V, et al. Isolation and characterization of a novel thermophile; Bacillus haynesii, applied for the green synthesis of ZnO nanoparticles. Artif Cells Nanomed Biotechnol. 2019;47(1):2072–82. https://doi.org/10.1080/21691401.2019.1620254.10.1080/21691401.2019.1620254 ↗
74. Berg JM, Romoser A, Banerjee N, Zebda R, Sayes CM. The relationship between pH and zeta potential of ∼ 30 nm metal oxide nanoparticle suspensions relevant to in vitro toxicological evaluations. Nanotoxicology. 2009;3(4):276–83. https://doi.org/10.3109/17435390903276941.10.3109/17435390903276941 ↗
75. Iqtedar M, Riaz H, Kaleem A, Abdullah R, Aihetasham A, Naz S, et al. Biosynthesis, optimization and characterization of ZnO nanoparticles using Bacillus cereus MN181367 and their antimicrobial activity against multidrug resistant bacteria. Rev Mex Ing Quim. 2020;19(Sup 1):253–66.10.24275/rmiq/Bio1605 ↗
76. Abdelhakim HK, El-Sayed ER, Rashidi FB. Biosynthesis of zinc oxide nanoparticles with antimicrobial, anticancer, antioxidant and photocatalytic activities by the endophytic Alternaria tenuissima. J Appl Microbiol. 2020;128(6):1634–46. https://doi.org/10.1111/jam.14581.10.1111/jam.14581 ↗
77. Ramachandran T, Manoharan D, Natesan S, Rajaram SK, Karuppiah P, Shaik MR, et al. Synthesis and structural characterization of selenium nanoparticles–Bacillus sp. MKUST-01 exopolysaccharide (SeNPs–EPS) conjugate for biomedical applications. Biomedicines. 2023;11(9):2520. https://doi.org/10.3390/biomedicines11092520.10.3390/biomedicines11092520 ↗
78. Kyu Aung K, Thida Htun K. Preparations and characterizations of chitosan, ZnO nanoparticle and chitosan-ZnO nanocomposite. J Myanmar Acad Arts Sci. 2020;18:247–258.
79. Ali SA, Ali ES, Hamdy G, Badawy MSEM, Ismail AR, El-Sabbagh IA, et al. Enhancing physical characteristics and antibacterial efficacy of chitosan through investigation of microwave-assisted chemically formulated chitosan-coated ZnO and chitosan/ZnO physical composite. Sci Rep. 2024;14(1):9348. https://doi.org/10.1038/s41598-024-58862-6.10.1038/s41598-024-58862-6 ↗
80. Yassin MT, Mostafa AA-F, Al-Askar AA, Al-Otibi FO. Facile green synthesis of zinc oxide nanoparticles with potential synergistic activity with common antifungal agents against multidrug-resistant candidal strains. Cryst (Basel). 2022;12(6):774. https://doi.org/10.3390/cryst12060774.10.3390/cryst12060774 ↗
81. Benitez LL, Carver PL. Adverse effects associated with long-term administration of azole antifungal agents. Drugs. 2019;79(8):833–53. https://doi.org/10.1007/s40265-019-01127-8.10.1007/s40265-019-01127-8 ↗
82. Bellanger X, Schneider R, Dezanet C, Arroua B, Balan L, Billard P, et al. Zn2+ leakage and photo-induced reactive oxidative species do not explain the full toxicity of ZnO core quantum Dots. J Hazard Mater. 2020;396:122616. https://doi.org/10.1016/j.jhazmat.2020.122616.10.1016/j.jhazmat.2020.122616 ↗
83. Wright GD, Walsh CT. D-alanyl-D-alanine ligases and the molecular mechanism of vancomycin resistance. Acc Chem Res. 1992;25(10):468–73. https://doi.org/10.1021/ar00022a006.10.1021/ar00022a006 ↗
84. Baka ZA, Abou-Dobara MI, El-Sayed AKA, El-Zahed MM. Synthesis, characterization and antimicrobial activity of chitosan/Ag nanocomposite using Escherichia coli D8. Sci J Damietta Fac Sci. 2019;9(1):1–6. https://doi.org/10.21608/sjdfs.2019.194816.10.21608/sjdfs.2019.194816 ↗
85. El-Zahed MM, Abou-Dobara MI, El-Khodary MM, Mousa MMA. Antimicrobial activity and nanoremediation of heavy metals using biosynthesized CS/GO/ZnO nanocomposite by Bacillus subtilis ATCC 6633 alone or immobilized in a macroporous cryogel. Microb Cell Fact. 2024;23(1):278. https://doi.org/10.1186/s12934-024-02535-6.10.1186/s12934-024-02535-6 ↗
86. El-Zahed MM, Baka ZAM, Abou-Dobara MI, El-Sayed AKA. Biogenic synthesis of chitosan/silver nanocomposite by Escherichia coli D8 (MF062579) and its antibacterial activity. Jordan J Biol Sci. 2023;16(2):279–88.10.54319/jjbs/160212 ↗
87. Govindasamy GA, Mydin RBSMN, Harun NH, Effendy WNFWE, Sreekantan S. Giant milkweed plant-based copper oxide nanoparticles for wound dressing application: physicochemical, bactericidal and cytocompatibility profiles. Chem Pap. 2023;77(2):1181–200. https://doi.org/10.1007/s11696-022-02513-5.10.1007/s11696-022-02513-5 ↗
88. Saravanabava J, Raja K, Indirani BS, Jayaseelan R, Kamalakkannan J. Characterization and applications of a novel semiconductor ZnO–CuCrO nanocomposite material through the co-precipitation method. 2022. p. 050003. https://doi.org/10.1063/5.007682810.1063/5.0076828 ↗
89. Govindasamy GA, Sreekantan S, Saharudin KA, Poliah R, Ong MT, Thavamany PJ, et al. Composition-dependent physicochemical and bactericidal properties of dual Cu–TiO2 nanoparticles incorporated in polypropylene. Bionanoscience. 2024;14(2):770–82. https://doi.org/10.1007/s12668-024-01339-7.10.1007/s12668-024-01339-7 ↗
90. Govindasamy GA, Sreekantan S, Saharudin KA, Ong MT, Thavamany PJ, Sahgal G, et al. Effect of compositions and heat treatments of polypropylene/PP-g-MAH/CuO–TiO2 composites on thermal, crystallization and antimicrobial properties. Bionanoscience. 2024;14(3):2678–90. https://doi.org/10.1007/s12668-024-01453-6.10.1007/s12668-024-01453-6 ↗
91. Dananjaya SHS, Kumar RS, Yang M, Nikapitiya C, Lee J, De Zoysa M. Synthesis, characterization of ZnO-chitosan nanocomposites and evaluation of its antifungal activity against pathogenic Candida albicans. Int J Biol Macromol. 2018;108:1281–8. https://doi.org/10.1016/j.ijbiomac.2017.11.046.10.1016/j.ijbiomac.2017.11.046 ↗
92. Wang Y, Zhang Q, Zhang C, Li P. Characterisation and cooperative antimicrobial properties of chitosan/nano-ZnO composite nanofibrous membranes. Food Chem. 2012;132(1):419–27. https://doi.org/10.1016/j.foodchem.2011.11.015.10.1016/j.foodchem.2011.11.015 ↗
93. Mahmoud LM, Ramadan AMM, Elfayoumy A, Soliman E, Mohammed MA. Antibacterial activity of zinc oxide nanoparticles against some multidrug-resistant strains of Escherichia coli and Staphylococcus aureus isolated from milk and milk products. Menoufia Vet Med J. 2025;1(1):1–16. https://doi.org/10.21608/vmmj.2024.330449.1019.10.21608/vmmj.2024.330449.1019 ↗
94. El-Khawaga AM, Elsayed MA, Gobara M, Suliman AA, Hashem AH, Zaher AA, et al. Green synthesized ZnO nanoparticles by Saccharomyces cerevisiae and their antibacterial activity and photocatalytic degradation. Biomass Convers Biorefin. 2025;15(2):2673–84. https://doi.org/10.1007/s13399-023-04827-0.10.1007/s13399-023-04827-0 ↗
95. Mu Q, Jiang G, Chen L, Zhou H, Fourches D, Tropsha A, et al. Chemical basis of interactions between engineered nanoparticles and biological systems. Chem Rev. 2014;114(15):7740–81. https://doi.org/10.1021/CR400295A.10.1021/CR400295A ↗
96. Ivask A, Bondarenko O, Jepihhina N, Kahru A. Profiling of the reactive oxygen species-related ecotoxicity of CuO, ZnO, TiO2, silver and fullerene nanoparticles using a set of Recombinant luminescent Escherichia coli strains: differentiating the impact of particles and solubilised metals. Anal Bioanal Chem. 2010;398(2):701–16.10.1007/s00216-010-3962-7 ↗
97. El-Fawal MF, El-Fallal AA, Abou-Dobara MI, El-Sayed AKA, El-Zahed MM. Preparation, characterization, and antibacterial activity of newly biosynthesized ampicillin/chitosan/selenium nanocomposite (AMP/CS/SENC) using Fusarium fujikuroi PP794203 against multidrug-resistant Escherichia coli PP797596. J Microbiol Biotechnol Food Sci. 2024;14(3):e11608. https://doi.org/10.55251/jmbfs.11608.10.55251/jmbfs.11608 ↗
98. Dhlamini KS, Selepe CT, Ramalapa B, Tshweu L, Ray SS. Reimagining chitosan-based antimicrobial biomaterials to mitigate antibiotic resistance and alleviate antibiotic overuse: a review. Macromol Mater Eng. 2024;309(9):2400018. https://doi.org/10.1002/mame.202400018.10.1002/mame.202400018 ↗
99. Alfinaikh RS, Alamry KA, Hussein MA. Sustainable and biocompatible hybrid materials-based sulfated polysaccharides for biomedical applications: a review. RSC Adv. 2025;15(6):4708–67. https://doi.org/10.1039/D4RA07277D.10.1039/D4RA07277D ↗
100. Kausar R, Khan A, Jamil B, Shahzad Y, ul-Haq I. Development and pharmacological evaluation of vancomycin loaded chitosan films. Carbohydr Polym. 2021;256:117565. https://doi.org/10.1016/j.carbpol.2020.117565.10.1016/j.carbpol.2020.117565 ↗