Evaluation of silver nitroprusside nanoparticles: selective toxicity against MCF-7 cells and in vivo antitumor activity
Source abstract
Abstract
A number of different metallic nanoparticles have been extensively investigated in recent years owing to their diverse potential biomedical and cancer applications, antibacterial activities, and chemical properties. Here, silver nitroprusside nanoparticles (AgNPs) were prepared from silver nitrate and sodium nitroprusside, and their anticancer activity was evaluated. AgNPs were prepared and characterized using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray dispersive energy spectroscopy (EDX), and transmission electron microscopy (TEM). In vitro tests were performed using two breast cancer cell lines, a non-malignant breast epithelial cell line (MCF-10A), and a breast cancer cell line (MCF-7). The results obtained from cytotoxicity assays (MTT and resazurin assays) and bright-field microscopy revealed that AgNPs (3.0 μg/mL) exhibited preferential selectivity for non-malignant breast epithelial cells and were toxic to tumorigenic cells (MCF-7), with preferential cell death via apoptosis. Furthermore, in vivo experiments using a murine breast tumor model demonstrated that AgNP treatment significantly inhibited tumor growth without systemic toxicity, confirming its selective antitumor activity. These results suggest that AgNPs have potential applications in breast cancer treatment. In this study, we focused on the development and application of silver nitroprusside nanoparticles in in vitro and in vivo models to evaluate their antitumor activity and effects in different models of breast cancer.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Nanoparticles: synthesis and applications · Metal complexes synthesis and properties · Nanoparticle-Based Drug Delivery
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (67)
Show source-provided references
1. Khan I, Saeed K, Khan I. Nanoparticles: properties, applications and toxicities. Arab J Chem. 2019;12:908–31.10.1016/j.arabjc.2017.05.011 ↗
2. Ryabchikova E. Advances in nanomaterials in biomedicine. Nanomater. 2021;11:1–5. https://doi.org/10.3390/nano11010118.10.3390/nano11010118 ↗
3. Aziz SG-G, Aziz SG-G, Akbarzadeh A. Advances in Silver Nanotechnology: an update on biomedical applications and future perspectives. Drug Res. 2017;67:198–203. https://doi.org/10.1055/s-0042-112810.10.1055/s-0042-112810 ↗
4. Anjum S, Ishaque S, Fatima H, Farooq W, Hano C, Abbasi BH, et al. Emerging applications of nanotechnology in healthcare systems: grand challenges and perspectives. Pharmaceuticals. 2021;14:1–27. https://doi.org/10.3390/ph14080707.10.3390/ph14080707 ↗
5. Janicka M, Gubernator J. Use of nanotechnology for improved pharmacokinetics and activity of immunogenic cell death inducers used in cancer chemotherapy. Expert Opin Drug Deliv. 2017;14:1059–75. https://doi.org/10.1080/17425247.2017.1266333.10.1080/17425247.2017.1266333 ↗
6. Kumar A, Pandey AN, Jain SK. Nasal-nanotechnology: revolution for efficient therapeutics delivery. Drug Deliv. 2016;23:671–83. https://doi.org/10.3109/10717544.2014.920431.10.3109/10717544.2014.920431 ↗
7. Saka R, Chella N. Nanotechnology for delivery of natural therapeutic substances: a review. Environ Chem Lett. 2021;19:1097–106. https://doi.org/10.1007/s10311-020-01103-9.10.1007/s10311-020-01103-9 ↗
8. Sindhwani S, Chan WCW. Nanotechnology for modern medicine: next step towards clinical translation. J Intern Med. 2021;290:486–98. https://doi.org/10.1111/joim.13254.10.1111/joim.13254 ↗
9. World Health Organization (WHO). Breast Cancer: Fact Sheet. World Health Organization, Geneva, Switzerland, 2023. Available at: https://www.who.int/news-room/fact-sheets/detail/breast-cancer
10. International Agency for Research on Cancer (IARC). Press Release No. 361: IARC Global Cancer Observatory Predicts More Than 35 Million New Cancer Cases in 2050. February 2025. Available at: https://www.iarc.who.int/wp-content/uploads/2025/02/pr361_E.pdf
11. Obidiro O, Battogtokh G, Akala EO. Triple negative breast cancer treatment options and limitations: future outlook. Pharmaceutics. 2023;15:1796. https://doi.org/10.3390/pharmaceutics15071796.10.3390/pharmaceutics15071796 ↗
12. Surya C, Lakshminarayana ABV, Ramesh SH, Kunjiappan S, Theivendren P, Kumar ASK, et al. Advancements in breast cancer therapy: the promise of copper nanoparticles (CuNPs). J Trace Elem Med Biol. 2024;86:127526. https://doi.org/10.1016/j.arabjc.2021.103246.10.1016/j.arabjc.2021.103246 ↗
13. Aw MS, Kurian M, Losic D. Polymeric micelles for multidrug delivery and combination therapy. Chem Eur J. 2013;19:12586–601. https://doi.org/10.1002/chem.201302097.10.1002/chem.201302097 ↗
14. Ventola CL. The nanomedicine revolution: part 1: emerging concepts. Pharm Ther. 2012;37:512–25.
15. Mitchell MJ, Billingsley MM, Haley RM, Wechsler ME, Peppas NA, Langer R. Engineering precision nanoparticles for drug delivery. Nat Rev Drug Discov. 2021;20:101–24. https://doi.org/10.1038/s41573-020-0090-8.10.1038/s41573-020-0090-8 ↗
16. Yao Y, Zhou Y, Liu L, Xu Y, Chen Q, Wang Y, et al. Nanoparticle-based drug delivery in cancer therapy and its role in overcoming drug resistence. Front Mol Biosci. 2020;7:1–14. https://doi.org/10.3389/fmolb.2020.00193.10.3389/fmolb.2020.00193 ↗
17. Li X, Jian M, Sun Y, Zhu Q, Wang Z. The peptide functionalized inorganic nanoparticles for cancer-related bionalytical and biomedical applications - review. Molecules. 2021;26:3228–58. https://doi.org/10.3390/molecules26113228.10.3390/molecules26113228 ↗
18. Sun M, Wang T, Li L, Li X, Zhai Y, Zhang J, et al. The application of inorganic nanoparticles in molecular targeted cancer therapy: EGFR targeting. Front Pharmacol. 2021;12:1–15. https://doi.org/10.3389/fphar.2021.702445.10.3389/fphar.2021.702445 ↗
19. Song W, Jia P, Zhang T, Dou K, Liu L, Ren Y, et al. Cell membrane-camouflaged inorganic nanoparticles for cancer therapy—review. J Nanobiotechnol. 2022;20:1–29. https://doi.org/10.1186/s12951-022-01475-w.10.1186/s12951-022-01475-w ↗
20. Li H, Qu X, Qian W, Song Y, Wang C, Liu W. Andrographolide-loaded solid lipid nanoparticles enhance anticancer activity against head and neck cancer and precancerous cells. Oral Dis. 2022;28:142–9. https://doi.org/10.1111/odi.13751.10.1111/odi.13751 ↗
21. Akanda M, Getti G, Nandi U, Mithu MS, Douroumis D. Bioconjugated solid lipid nanoparticles (SLNs) for targeted prostate cancer therapy. Int J Pharm. 2021;599:120416–28. https://doi.org/10.1016/j.ijpharm.2021.120416.10.1016/j.ijpharm.2021.120416 ↗
22. Grewal IK, Singh S, Arora S, Sharma N. Polymeric nanoparticles for breast cancer therapy: a comprehensive review. Biointerface Res Appl Chem. 2021;11:11151–71. https://doi.org/10.33263/BRIAC114.1115111171.10.33263/BRIAC114.1115111171 ↗
23. C.F. Freitas, A.L. Tessaro, D.S. Pellosi, (2022) Polymeric Nanoparticles for Treranostic Treatment of Cancer, S. Jana, S. Jana (Eds), Functional Biomaterials, Drug Delivery and Biomedical Applications. Springer, Singapore. (pp. 149-185)10.1007/978-981-16-7152-4_6 ↗
24. Jayme CC, Pires AF, Fernandes DS, Tedesco AC. DNA polymer films used as drug delivery systems to early-stage diagnose and treatment of breast cancer using 3D tumor spheroids as a model. Photodiagnosis Photodyn Ther. 2022;37:102575–87. https://doi.org/10.1016/j.pdpdt.2021.102575.10.1016/j.pdpdt.2021.102575 ↗
25. Sokolov K, Tam J, Travis K, Larson T, Aaron J, Harrison N, et al. Cancer imaging and therapy with metal nanoparticles. IEEE Eng Med Biol Soc. 2009. https://doi.org/10.1109/IEMBS.2009.5333430.10.1109/IEMBS.2009.5333430 ↗
26. Fekrazad R, Naghdi N, Nokhbatolfoghahaei H, Bagheri H. The combination of laser therapy and metal nanoparticles in cancer treatment originated from epithelial tissues: a literature review. J Lasers Med Sci. 2016;7:62–75. https://doi.org/10.15171/jlms.2016.13.10.15171/jlms.2016.13 ↗
27. Parvanian S, Mostafavi SM, Aghashiri M. Multifunctional nanoparticle developments in cancer diagnosis and treatment. Sens Biosens Res. 2017;13:81–7. https://doi.org/10.1016/j.sbsr.2016.08.002.10.1016/j.sbsr.2016.08.002 ↗
28. Kovács D, Igaz N, Keskeny C, Bélteky P, Tóth T, Gáspár R, et al. Silver nanoparticles defeat p53-positive and p53-negative osteosarcoma cells by triggering mitochondrial stress and apoptosis. Sci Rep. 2016;6:1–13. https://doi.org/10.1038/srep27902.10.1038/srep27902 ↗
29. Rao BR, Kotcherlakota R, Nethi SK, Puvvada N, Bojja N, Chaudhuri A, et al. Ag2[Fe(CN)5NO] nanoparticles exhibit anti-bacterial activity and wound healing properties. ACS Biomater Sci Eng. 2018. https://doi.org/10.1021/acsbiomaterials.8b00759.10.1021/acsbiomaterials.8b00759 ↗
30. Swanner J, Fahrenholtz CD, Tenvooren I, Bernish BW, Sears JJ, Hooker A, et al. Silver nanoparticles selectively treat triple-negative breast cancer cells without affecting non-malignant breast epithelial cells in vitro and in vivo. FASEB BioAdv. 2019. https://doi.org/10.1096/fba.2019-00021.10.1096/fba.2019-00021 ↗
31. Faheem MM, Bhagat M, Sharma P, Anand R. Induction of p53mediated mitochondrial apoptosis and cell cycle arrest in human breast cancer cells by plant mediated synthesis of silver nanoparticles from Bergenia ligulata (whole plant). Int J Pharm. 2022;619:121710–23. https://doi.org/10.1016/j.ijpharm.2022.121710.10.1016/j.ijpharm.2022.121710 ↗
32. Medici S, Peana M, Crisponi G, Nurchi VM, Lachowicz JI, Remelli M, et al. Silver coordination compounds: a new horizon in medicine. Coord Chem Rev. 2016;327:349–59. https://doi.org/10.1016/j.ccr.2016.05.015.10.1016/j.ccr.2016.05.015 ↗
33. Blanco J, Lafuente D, Gómez M, García T, Domingo JL, Sánchez DJ. Polyvinyl pyrrolidone-coated silver nanoparticles in a human lung cancer cells: time-and dose-dependent influence over p53 and caspase-3 protein expression and epigenetic effects. Arch Toxicol. 2017;91:651–66. https://doi.org/10.1007/s00204-016-1773-0.10.1007/s00204-016-1773-0 ↗
34. Thapa RK, Kim JH, Jeong JH, Shin BS, Choi HG, Yong CS, et al. Silver nanoparticle-embedded graphene oxide-methotrexate for targeted cancer treatment. Colloids Surf B Biointerfaces. 2017;153:95–103. https://doi.org/10.1016/j.colsurfb.2017.02.012.10.1016/j.colsurfb.2017.02.012 ↗
35. Gomes HIO, Martins CSM, Prior JAV. Silver nanoparticles as carriers of anticancer drugs for efficient target treatment of cancer cells. Nanomater. 2021;11:964–95. https://doi.org/10.3390/nano11040964.10.3390/nano11040964 ↗
36. Miranda RR, Sampaio I, Zucolotto V. Exploring silver nanoparticles for cancer therapy and diagnosis. Colloids Surf B Biointerfaces. 2022;210:112254–67. https://doi.org/10.1016/j.colsurfb.2021.112254.10.1016/j.colsurfb.2021.112254 ↗
37. Choudhari SK, Chaudhary M, Bagde S, Gadbail AR, Joshi V. Nitric oxide and cancer: a review. World J Surg Oncol. 2013;11:2–11. https://doi.org/10.1186/1477-7819-11-118.10.1186/1477-7819-11-118 ↗
38. Yang Y, Huang Z, Li L. Advanced nitric oxide donors: chemical structure of NO drugs, NO nanomedicines and biomedical applications. Nanoscale. 2021;13:444–59. https://doi.org/10.1039/D0NR07484E.10.1039/D0NR07484E ↗
39. Sahebnasagh A, Saghafi F, Negintaji S, Hu T, Shabani-Borujeni M, Safdari M, et al. Nitric Oxide and Immune Responses in Cancer: Searching for New Therapeutic Strategies. Curr Med Chem. 2022;29:1561–95. https://doi.org/10.2174/0929867328666210707194543.10.2174/0929867328666210707194543 ↗
40. Thomas DD, Ridnour LA, Isenberg JS, Flores-Santana W, Switzer CH, Donzellie S, et al. The chemical biology of nitric oxide. implications in cellular signaling. Free Rad Biol Med. 2008;45:18–31. https://doi.org/10.1016/j.freeradbiomed.2008.03.020.10.1016/j.freeradbiomed.2008.03.020 ↗
41. Burke AJ, Sullivan FJ, Giles FJ, Glynn SA. The yin and yang of nitric oxide in cancer progression. Carcinogenesis. 2013;34:503–12. https://doi.org/10.1093/carcin/bgt034.10.1093/carcin/bgt034 ↗
42. Fukumura D, Kashiwagi S, Jain RK. The role of nitric oxide in tumour progression. Nat Rev Cancer. 2006;6:521–34. https://doi.org/10.1038/nrc1910.10.1038/nrc1910 ↗
43. Cardnell RJ, Mikkelsen RB. Nitric oxide synthase inhibition enhances the antitumor effect of radiation in treating squamous carcinoma xenografts. PLoS ONE. 2011;6:1–9. https://doi.org/10.1371/journal.pone.0020147.10.1371/journal.pone.0020147 ↗
44. Vo V, Van MN, Lee HI, Kim JM, Kim Y, Kim SJ. A new route for obtaining Prussian blue nanoparticles. Mater Chem Phys. 2008;107:6–8. https://doi.org/10.1016/j.matchemphys.2007.07.002.10.1016/j.matchemphys.2007.07.002 ↗
45. Dos Santos VS, Maraldi VA, Bonfim KS, Souza TR, Nakamura APR, Magossi MS, et al. Voltammetric behavior of zinc hexacyanoferrate (III) nanoparticles and their application in the detection of N-acetylcysteine. Int J Electrochem Sci. 2017;12:7142–53. https://doi.org/10.20964/2017.08.06.10.20964/2017.08.06 ↗
46. Rezende N, Jayme CC, Brassesco MS, Tedesco AC, Oliveira HF. Standardization of a resazurin-based assay for the evaluation of metabolic activity in oral squamous carcinoma and glioblastoma cells. Photodiagnosis Photodyn Ther. 2019;26:371–4. https://doi.org/10.1016/j.pdpdt.2019.05.004.10.1016/j.pdpdt.2019.05.004 ↗
47. Le Naour A, Rossary A, Vasson MP. EO771, is it a well-characterized cell line for mouse mammary cancer model? Limit and uncertainty. Cancer Med. 2020;9:8074–85. https://doi.org/10.1002/cam4.3295.10.1002/cam4.3295 ↗
48. Jayme CC, Pires AF, Tedesco AC. Development of DNA polymer films as a drug delivery system for the treatment of oral cancer. Drug Deliv Transl Res. 2020;10:1612–25. https://doi.org/10.1007/s13346-020-00801-9.10.1007/s13346-020-00801-9 ↗
49. Patterson AL. The Scherrer formula for X-ray particle size determination. Phys Rev. 1939;56:978–82. https://doi.org/10.1103/PhysRev.56.978.10.1103/PhysRev.56.978 ↗
50. Bilgic E, Tuncel N, Koca T. Radio-sensitivity on MCF-7 cells of silver nanoparticles synthesized by Silybum marianum. Inorg Nano-Metal Chem. 2021. https://doi.org/10.1080/24701556.2021.1987460.10.1080/24701556.2021.1987460 ↗
51. Slator C, Molphy Z, McKee V, Long C, Brown T, Kellett A. Di-copper metallodrugs promote NCI-60 chemotherapy via singlet oxygen and superoxide production with tandem TA/TA and AT/AT oligonucleotide discrimination. Nucleic Acids Res. 2018;46:2733–50. https://doi.org/10.1093/nar/gky105.10.1093/nar/gky105 ↗
52. Upanan S, Yodkeeree S, Thippraphan P, Punfa W, Wongpoomchai R, Limtrakul P. The proanthocyanidin-rich fraction obtained from red rice germ and bran extract induces HepG2 hepatocellular carcinoma cell apoptosis. Molecules. 2019;24:813. https://doi.org/10.3390/molecules24040813.10.3390/molecules24040813 ↗
53. Murabayashi D, Mochizuki M, Tamaki Y, Nakahara T. Practical methods for handling human periodontal ligament stem cells in serum-free and serum-containing culture conditions under hypoxia: implications for regenerative medicine. Hum Cell. 2017;30:169–80. https://doi.org/10.1007/s13577-017-0161-2.10.1007/s13577-017-0161-2 ↗
54. Mochizuki M, Nakahara T. Establishment of xenogeneic serum-free culture methods for handling human dental pulp stem cells using clinically oriented in-vitro and in-vivo conditions. Stem Cell Res Ther. 2018;9:25. https://doi.org/10.1186/s13287-018-1011-1.10.1186/s13287-018-1011-1 ↗
55. Nachat-Kappes R, Pinel A, Combe K, Lamas B, Farges M-C, Rossary A, et al. Effects of enriched environment on COX-2, leptin and eicosanoids in a mouse model of breast cancer. PLoS ONE. 2012;7:e51525–39. https://doi.org/10.1371/journal.pone.0051525.10.1371/journal.pone.0051525 ↗
56. Mishra D, Patel V, Banerjee D. Nitric oxide and S-nitrosylation in cancers: emphasis on breast cancer. Breast Cancer Basic Clin Res. 2020;14:1–9. https://doi.org/10.1177/1178223419882688.10.1177/1178223419882688 ↗
57. Putti TC, Pinder SE, Elston CW, Lee AHS, Ellis IO. Breast pathology practice: most common problems in a consultation service. Histopathology. 2005;47:445–57. https://doi.org/10.1111/j.1365-2559.2005.02246.x.10.1111/j.1365-2559.2005.02246.x ↗
58. Ahmeda A, Mahdavi B, Zaker F, Kaviani S, Hosseini S, Zangeneh MM, et al. Green formulation and chemical characterization of Lens culinaris seed aqueous extract conjugated gold nanoparticles for the treatment of acute myeloid leukemia in comparison to mitoxantrone in a leukemic mouse model. Appl Organomet Chem. 2020;34:e5369. https://doi.org/10.1002/aoc.5369.10.1002/aoc.5369 ↗
59. Nejad FS, Haghi M, Alizade-Harakiyan M, Ahmadi A. Investigating the efficacy of silver Anethum graveolens nano-extract in colon cancer radiotherapy. ChemistrySelect. 2025;10:e202405345. https://doi.org/10.1002/slct.202405345.10.1002/slct.202405345 ↗
60. Abbasi N, Ghaneialvar H, Moradi R, Zangeneh MM, Zangeneh A. Formulation and characterization of a novel cutaneous wound healing ointment by silver nanoparticles containing Citrus lemon leaf: a chemobiological study. Arab J Chem. 2021;14:103246. https://doi.org/10.1016/j.arabjc.2021.103246.10.1016/j.arabjc.2021.103246 ↗
61. Ahmeda A, Zangeneh A, Zangeneh MM. Characterization and anti-acute T cell leukemia properties of silver nanoparticles synthesized by a green approach for bioremediation applications: introducing a new chemotherapeutic drug for clinical trial studies. Appl Organomet Chem. 2020;34:e5374. https://doi.org/10.1002/aoc.5374.10.1002/aoc.5374 ↗
62. Bai J, Gongsun X, Xue L, Zangeneh MM. Introducing a modern chemotherapeutic drug formulated by iron nanoparticles for the treatment of human lung cancer. J Exp Nanosci. 2021;16:397–409. https://doi.org/10.1080/17458080.2021.1998460.10.1080/17458080.2021.1998460 ↗
63. Li Y, Li N, Jiang W, Ma G, Zangeneh MM. In situ decorated Au NPs on pectin-modified Fe3O4 NPs as a novel magnetic nanocomposite (Fe3O4/Pectin/Au) for catalytic reduction of nitroarenes and investigation of its anti-human lung cancer activities. Int J Biol Macromol. 2020;163:2162–71. https://doi.org/10.1016/j.ijbiomac.2020.09.102.10.1016/j.ijbiomac.2020.09.102 ↗
64. J. Liu; A. Zangeneh; M.M. Zangeneh; B. Guo Antioxidant, cytotoxicity, anti-human esophageal squamous cell carcinoma, anti-human Caucasian esophageal carcinoma, anti-adenocarcinoma of the gastroesophageal junction and anti-distal esophageal adenocarcinoma properties of gold nanoparticles green-synthesized by Rhus coriaria L. fruit aqueous extract. J. Exp. Nanosci. 15 (2020) 202–216. https://doi.org/10.1080/17458080.2020.176667510.1080/17458080.2020.1766675 ↗
65. Zangeneh A, Zangeneh MM, Moradi R. Ethnomedicinal plant-extract-assisted green synthesis of iron nanoparticles using Allium saralicum extract, and their antioxidant, cytotoxicity, antibacterial, antifungal and cutaneous wound-healing activities. Appl Organomet Chem. 2020;34:e5247. https://doi.org/10.1002/aoc.5247.10.1002/aoc.5247 ↗
66. Han Y, Gao Y, Cao X, Zangeneh MM, Liu S, Li J. Ag NPs on chitosan-alginate coated magnetite for synthesis of indazolo[2,1-b]phthalazines and human lung protective effects against α-Guttiferin. Int J Biol Macromol. 2020;164:2974–86. https://doi.org/10.1016/j.ijbiomac.2020.08.183.10.1016/j.ijbiomac.2020.08.183 ↗
67. Ryan J, Jacob P, Lee A, Gagnon Z, Pavel IE. Biodistribution and toxicity of antimicrobial ionic silver (Ag+) and silver nanoparticle (AgNP+) species after oral exposure, in Sprague-Dawley rats. Food Chem Toxicol. 2022;166:113228–39. https://doi.org/10.1016/j.fct.2022.113228.10.1016/j.fct.2022.113228 ↗