Copper-doped zinc oxide nanoparticles as efficient antimicrobial agents: bridging electronic properties with biological properties
Source abstract
Abstract
Zinc oxide nanoparticles (ZnO NPs) have already shown potential applications as antimicrobial agents. However, the large band gap and charge carrier recombination of ZnO NPs reduce the production of reactive oxygen species, which limits their antibacterial activity; therefore, further modifications of their structural and electronic properties are required. Herein, Cu doping of ZnO NPs has been carried out by a microwave-assisted method to modify their structural, electronic, and biological properties. The structural and morphological analysis of the prepared Cu-doped ZnO NPs confirmed that Cu is well incorporated into the ZnO NPs lattice, and the morphology is quasi-spherical with a diameter range of 20-25 nm. The changes in the band gap and defect states in ZnO NPs, formed upon Cu doping, were confirmed by UV-visible and theoretical analysis. The antimicrobial potential of ZnO and Cu-doped ZnO NPs against S. aureus, E. coli, P. aeruginosa, and C. albicans has been evaluated by measuring the zone of inhibition, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The results showed an enhancement of the antimicrobial potential of ZnO NPs upon Cu doping, which could be related to modifications of the electronic structure that could potentially affect redox properties.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: ZnO doping and properties · Nanoparticles: synthesis and applications · Infectious Aortic and Vascular Conditions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (41)
Show source-provided references
1. Coque TM, Cantón R, Pérez-Cobas AE, Fernández-de-Bobadilla MD, Baquero F. Antimicrobial resistance in the global health network: known unknowns and challenges for efficient responses in the 21st century. Volume 11. Microorganisms; 2023. p. 1050.10.3390/microorganisms11041050 ↗
2. Elfadadny A, Ragab RF, AlHarbi M, Badshah F, Ibáñez-Arancibia E, Farag A, Hendawy AO, De los Ríos-Escalante PR, Aboubakr M, Zakai SA. Antimicrobial resistance of Pseudomonas aeruginosa: navigating clinical impacts, current resistance trends, and innovations in breaking therapies. Front Microbiol. 2024;15:1374466.10.3389/fmicb.2024.1374466 ↗
3. Sati H, Carrara E, Savoldi A, Hansen P, Garlasco J, Campagnaro E, Boccia S, Castillo-Polo JA, Magrini E, Garcia-Vello P. The WHO Bacterial Priority Pathogens List 2024: a prioritisation study to guide research, development, and public health strategies against antimicrobial resistance. Lancet Infect Dis. 2025;25:1033–43.10.1016/S1473-3099(25)00118-5 ↗
4. Patel JK, Patel A, Bhatia D. Introduction to nanomaterials and nanotechnology, Emerging technologies for nanoparticle manufacturing. Springer; 2021. pp. 3–23.10.1007/978-3-030-50703-9_1 ↗
5. Ussia M, Privitera V, Scalese S. Unlocking the potential and versatility of quantum dots: from biomedical to environmental applications and smart micro/nanorobots. Adv Mater Interfaces. 2024;11:2300970.10.1002/admi.202300970 ↗
6. Lebaka VR, Ravi P, Reddy MC, Thummala C, Mandal TK. Zinc oxide nanoparticles in modern science and technology: multifunctional roles in healthcare, environmental remediation, and industry. Nanomaterials. 2025;15:754.10.3390/nano15100754 ↗
7. Xie J, Li H, Zhang T, Song B, Wang X, Gu Z. Recent advances in ZnO nanomaterial-mediated biological applications and action mechanisms. Nanomaterials. 2023;13:1500.10.3390/nano13091500 ↗
8. Al-Naymi HAS, Al-Musawi MH, Mirhaj M, Valizadeh H, Momeni A, Pajooh AMD, Shahriari-Khalaji M, Sharifianjazi F, Tavamaishvili K, Kazemi N. Exploring nanobioceramics in wound healing as effective and economical alternatives. Heliyon. 2024;10:e38497.10.1016/j.heliyon.2024.e38497 ↗
9. Puspasari V, Ridhova A, Hermawan A, Amal MI, Khan MM. ZnO-based antimicrobial coatings for biomedical applications. Bioprocess Biosyst Eng. 2022;45:1421–45.10.1007/s00449-022-02733-9 ↗
10. Danial EN, Hjiri M, Abdel-Wahab MS, Alonizan N, El Mir L, Aida M. Antibacterial activity of In-doped ZnO nanoparticles. Inorg Chem Commun. 2020;122:108281.10.1016/j.inoche.2020.108281 ↗
11. Archana P, Janarthanan B, Bhuvana S, Rajiv P, Sharmila S. Concert of zinc oxide nanoparticles synthesized using Cucumis melo by green synthesis and the antibacterial activity on pathogenic bacteria. Inorg Chem Commun. 2022;137:109255.10.1016/j.inoche.2022.109255 ↗
12. Iheme CI, Elemike EE, Igwe CU, Ujowundu FN, Ogbonna CU, Uche ZC, Okoro OC, Hossain I, Onwudiwe DC. Synthesis, characterization, radical scavenging properties of zinc oxide nanoparticles and inhibitory effect of ZnONPs-ciprofloxacin nanoconjugates on multidrug-resistant Staphylococcus aureus (MRSA) enzyme. Inorg Chem Commun. 2024;160:111864.10.1016/j.inoche.2023.111864 ↗
13. Islam F, Shohag S, Uddin MJ, Islam MR, Nafady MH, Akter A, Mitra S, Roy A, Emran TB, Cavalu S. Exploring the journey of zinc oxide nanoparticles (ZnO-NPs) toward biomedical applications. Materials. 2022;15:2160.10.3390/ma15062160 ↗
14. Lakshmi Prasanna V, Vijayaraghavan R. Insight into the mechanism of antibacterial activity of ZnO: surface defects mediated reactive oxygen species even in the dark. Langmuir. 2015;31:9155–62.10.1021/acs.langmuir.5b02266 ↗
15. Ayanwale AP, Estrada-Capetillo BL, Reyes-López SY. Antifungal activity and cytotoxicity study of ZrO2-ZnO bimetallic nanoparticles. Inorg Chem Commun. 2021;134:108954.10.1016/j.inoche.2021.108954 ↗
16. Rao SM, Kotteeswaran S, Visagamani AM. Green synthesis of zinc oxide nanoparticles from camellia sinensis: Organic dye degradation and antibacterial activity. Inorg Chem Commun. 2021;134:108956.10.1016/j.inoche.2021.108956 ↗
17. Rani N, Rani S, Patel H, Yadav S, Saini M, Rawat S, Saini K. Characterization and investigation of antioxidant and antimicrobial activity of zinc oxide nanoparticles prepared using leaves extract of Nyctanthes arbor-tristis. Inorg Chem Commun. 2023;150:110516.10.1016/j.inoche.2023.110516 ↗
18. Tuama AN, Alzubaidi LH, Jameel MH, Abass KH, bin Mayzan MZH, Salman ZN. Impact of electron–hole recombination mechanism on the photocatalytic performance of ZnO in water treatment: A review. J Solgel Sci Technol. 2024;110:792–806.10.1007/s10971-024-06385-x ↗
19. Guntu RK. Photocatalysis and irradiative TL defect center analysis studies of Cu2 + ionic ZnO nano lattice. J Mol Liq. 2024;401:124685.10.1016/j.molliq.2024.124685 ↗
20. Nisar P, Ali N, Rahman L, Ali M, Shinwari ZK. Antimicrobial activities of biologically synthesized metal nanoparticles: an insight into the mechanism of action. J Biol Inorg Chem. 2019;24:929–41.10.1007/s00775-019-01717-7 ↗
21. Zhang B, Yang Z, Yang S, Xu Y, Tang X, Mao H, Dai R, Liang X. Construction of ZnO/Cu2O composites for enhanced antibacterial activity and analysis of antibacterial mechanism. Inorg Chem Commun. 2024;162:112182.10.1016/j.inoche.2024.112182 ↗
22. Singh A, Wan F, Yadav K, Salvi A, Thakur P, Thakur A. Synergistic effect of ZnO nanoparticles with Cu2 + doping on antibacterial and photocatalytic activity. Inorg Chem Commun. 2023;157:111425.10.1016/j.inoche.2023.111425 ↗
23. Bhuyan T, Khanuja M, Sharma R, Patel S, Reddy M, Anand S, Varma A. A comparative study of pure and copper (Cu)-doped ZnO nanorods for antibacterial and photocatalytic applications with their mechanism of action. J Nanopart Res. 2015;17:288.10.1007/s11051-015-3093-3 ↗
24. Harish V, Ansari MM, Tewari D, Gaur M, Yadav AB, García-Betancourt M-L, Abdel-Haleem FM, Bechelany M, Barhoum A. Nanoparticle and nanostructure synthesis and controlled growth methods. Nanomaterials. 2022;12:3226.10.3390/nano12183226 ↗
25. Karimi M, Sadeghi E, Bigdeli SK, Zahedifar M. Synthesis, feasibility study of production of singlet oxygen and hydroxyl radical and performance in antibacterial activity of ZnS: Eu QDs. J Compos Compd. 2022;4:77–82.
26. Karimi M, Sadeghi E, Bigdeli SK, Zahedifar M. Optical properties, singlet oxygen, and free radical production ability with different UV irradiations and antimicrobial inhibitors against various bacterial species of ZnO: Eu nanoparticles. Radiat Phys Chem. 2023;212:111132.10.1016/j.radphyschem.2023.111132 ↗
27. Selmi A, Fkiri A, Bouslimi J, Besbes H. Improvement of dielectric properties of ZnO nanoparticles by Cu doping for tunable microwave devices. J Mater Sci: Mater Electron. 2020;31:18664–72.
28. Vidyasagar C, Hosamani G, Kariyajjanavar P, Yathisha R, Nayaka YA. One-pot microwave synthesis and effect of Cu2 + ions on structural properties of Cu-ZnO nano crystals. Mater Today: Proc. 2018;5:22171–80.
29. Manoharan RK, Mahalingam S, Gangadaran P, Ahn Y-H. Antibacterial and photocatalytic activities of 5-nitroindole capped bimetal nanoparticles against multidrug resistant bacteria. Colloids Surf B. 2020;188:110825.10.1016/j.colsurfb.2020.110825 ↗
30. Tsogoo A, Tsedev N, Gibaud A, Daniel P, Kassiba A, Fukuda M, Kusano Y, Azuma M, Tsogbadrakh N, Ragchaa G. Experimental and ab initio studies on the structural, magnetic, photocatalytic, and antibacterial properties of Cu-doped ZnO nanoparticles. RSC Adv. 2023;13:1256–66.10.1039/D2RA07204A ↗
31. Khan MM, Harunsani MH, Tan AL, Hojamberdiev M, Poi YA, Ahmad N. Antibacterial studies of ZnO and Cu-doped ZnO nanoparticles synthesized using aqueous leaf extract of Stachytarpheta jamaicensis. BioNanoScience. 2020;10:1037–48.10.1007/s12668-020-00775-5 ↗
32. Ben Elkamel I, Hamdaoui N, Mezni A, Ajjel R, Beji L. Synthesis and characterization of Cu doped ZnO nanoparticles for stable and fast response UV photodetector at low noise current. J Mater Sci: Mater Electron. 2019;30:9444–54.
33. BM A, Dennison MS. Integrating microwave-assisted green synthesis, DFT simulations, and biological activity evaluation of copper-doped zinc oxide nanoparticles. Sci Rep. 2025;15:1–26.
34. Arunpandian M, Marnadu R, Kannan R, Kannan SK, Arputhavalli GJ, Arockiam SI, Mahmoud ZM, Shkir M, AlFaify S, Sreedevi G. Fabrication of Cu/ZnO system: a dual performer as photocatalyst and luminescent material. Inorg Chem Commun. 2021;134:109022.10.1016/j.inoche.2021.109022 ↗
35. Noman MT, Amor N, Petru M. Synthesis and applications of ZnO nanostructures (ZONSs): A review. Crit Rev Solid State Mater Sci. 2022;47:99–141.10.1080/10408436.2021.1886041 ↗
36. Saranya R, Mubarak Ali MB, Jeyalakshmi K. Enhanced catalytic and biological activities of Cu doped ZnO nanoparticles synthesized by microwave assisted method. Indian J Chem Technol (IJCT). 2022;29:288–95.
37. Prasad V, Simiyon GG, Mammen AE, Jayaprakash N. Microwave assisted synthesis, characterization and photo-catalytic study of Cu/ZnO nanocomposite. Rasayan J Chem. 2019;12:860–5.10.31788/RJC.2019.1225226 ↗
38. Van Den Berg R, Prieto G, Korpershoek G, Van Der Wal LI, Van Bunningen AJ, Lægsgaard-Jørgensen S, De Jongh PE, De Jong KP. Structure sensitivity of Cu and CuZn catalysts relevant to industrial methanol synthesis. Nat Commun. 2016;7:13057.10.1038/ncomms13057 ↗
39. Al-Obaidi MT, Rashid TM, Rahmah MI. Compositional tuning of Au/ZnO nanoparticles for superior antibacterial and photocatalytic activities. Trans Indian Inst Met. 2025;78:239.10.1007/s12666-025-03720-w ↗
40. Ahmed AM, Rahmah MI. Synthesis and evaluation of PVP-stabilized platinum-doped ZnO nanoparticles: A dual approach for photocatalytic and antibacterial applications. BioNanoScience. 2025;15:332.10.1007/s12668-025-01977-5 ↗
41. Rahmah MI, Qasim HB. A novel method to prepare antibacterial ZnO nanoflowers. Appl Phys A. 2022;128:998.10.1007/s00339-022-06161-0 ↗