DataVXAdvanced Functional MaterialsPaper
journal article·2025-07Open access

A Fully Automated Point‐of‐Care Device Using Organic Electrochemical Transistor‐Enhanced CRISPR/Cas12a for Amplification‐Free Nucleic Acid Detection

Jing Chen, Deqi Yang, Daizong Ji, Bihan Guo, Yuqian Guo, Huiping Lin, Ru Zhang, Zhiqiang Chang, Yuhan Lu, Guoqi Zhu, Lei Zhao, Thanyada Rungrotmongkol, Xinxin Lu, Qinjuan Ren, Wenjuan Wu, Ya Zhang, Yin Fang

Advanced Functional Materials· Volume 35· Issue 27
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Abstract

Abstract Conventional nucleic acid detection methods often involve complex, time‐consuming amplification procedures and rely on sophisticated equipment, rendering them incapable of serving end customers or community hospitals. Here, a miniaturized and automated point‐of‐care (POC) molecular diagnostic device is described that amplification‐free detects, via organic electrochemical transistors (OECTs) enhanced CRISPR/Cas12a system, achieving disease‐associated nucleic acids in real sample at attomolar level within 55 min. The device enables automated sample‐to‐answer analysis via the structural integration of a microfluidic control system, microfluidic chip, and wireless transmission module. Moreover, the device can rapidly distinguish severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) positive and negative patients with an accuracy of 97.4% using 13 pharyngeal swab samples. Besides, it is also shown that the device can detect various nucleic acid including in Staphylococcus aureus (S. aureus) and tumor cell by merely redesigning the gRNA regions of the CRISPR/Cas12a. Overall, this work provides an automated and customizable POC device that may facilitate diseases digital surveillance.
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Research context

Electronic materials

OpenAlex topics: CRISPR and Genetic Engineering · Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Research Center for Translational Medicine Medical Innovation Center and State Key Laboratory of Cardiology Shanghai East Hospital The Institute for Biomedical Engineering & Nano Science School of Medicine Tongji University Shanghai 200120 China

State Key Laboratory of Food Science and Resources School of Food Science and Technology Nanchang University Nanchang 330047 China

Department of Laboratory Medicine Shanghai East Hospital School of Medicine Tongji University Shanghai 200120 China

Tongji Hospital School of Medicine Tongji University Shanghai 200120 China

Center of Excellence in Biocatalyst and Sustainable Biotechnology Department of Biochemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand

Key Laboratory of Arrhythmias of the Ministry of Education of China Shanghai East Hospital affiliated to Tongji University Shanghai 200120 China

Funding

National Key Research and Development Program of China · National Natural Science Foundation of China · Fundamental Research Funds for the Central Universities · Science and Technology Commission of Shanghai Municipality

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