Lonicera japonica ‐Derived Nanovesicles Ameliorate Acute Lung Injury by Suppressing Neutrophil Extracellular Traps Formation in Mice
Source abstract
Abstract
ABSTRACT Acute lung injury (ALI) is a lethal respiratory disease. The inflammatory storm triggered by neutrophil extracellular traps (NETs) is the critical pathogenesis of pulmonary injury. But the effective clinical treatments remain limited for ALI. Hence, developing a pulmonary‐targeting nano‐drug is urgent in the clinic. Lonicera japonica ‐derived nanovesicles (LDNVs) was isolated and characterized. Distribution of LDNVs were enriched in the pulmonary neutrophils. LDNVs restored the body weight, physical activity and attenuated the pulmonary injury of LPS‐induced ALI mice. The inflammatory cytokines and neutrophil‐associated chemokines were decreased with LDNVs treatment. LDNVs also decreased pulmonary accumulation of neutrophils and NETs. The LC3B II was suppressed and p62 was enhanced by LDNVs treatment. Finally, LDNVs suppressed the translocation of LC3 in nuclear and lysosomal damage. But these therapeutic effects of LDNVs in ALI and NETs were both blocked by a neutrophils and NETs activator, phorbol myristate acetate (PMA). LDNVs attenuate LPS‐induced ALI by inhibiting NETs formation. These effects may be associated with LDNVs suppressing the autophagic flux.
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Research context
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Affiliations
The Third Clinical Medical College of Guangzhou University of Chinese Medicine Guangzhou University of Chinese Medicine Guangzhou China
The Third Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou University of Chinese Medicine Guangzhou China
School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine Guangzhou China
China Resources Sanjiu Medical & Pharmaceutical CO., LTD Shenzhen China
Artemisinin Research Center of Guangzhou University of Chinese Medicine Guangzhou University of Chinese Medicine Guangzhou China
The Second Affiliated Hospital of Guangzhou University of Chinese Medicine Guangzhou University of Chinese Medicine Guangzhou China
Sci‐tech Industrial Park Guangzhou University of Chinese Medicine Guangzhou China
Funding
National Natural Science Foundation of China
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