Poly(dimethylsiloxane) elastomers with tethered peptide ligands for cell adhesion studies
Source abstract
Abstract
Poly(dimethylsiloxane) (PDMS) is the choice of material for a wide range of biological and non-biological applications because of its chemical inertness, non-toxicity, ease of handling and commercial availability. However, PDMS exhibits uncontrolled protein adsorption and cell adhesion and it has proved difficult to functionalize to present bioactive ligands. We present a facile strategy for functional surface modification of PDMS using commercial reagents to engineer polymer brushes of oligo(ethylene glycol) methacrylate that prevent cell adhesion and can be functionalized to display bioadhesive ligands. The polymer brushes resist biofouling and prevent cell adhesion and bioadhesive peptides can be tethered either uniformly or constrained to micropatterned domains using standard peptide chemistry approaches. This approach is relevant to various biomedical and biotechnological applications.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Polymer Surface Interaction Studies · Nanofabrication and Lithography Techniques · Surface Modification and Superhydrophobicity
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (31)
Show source-provided references
1. Local force and geometry sensing regulate cell functions10.1038/nrm1890 ↗
2. Cell fate regulation by coupling mechanical cycles to biochemical signaling pathways10.1016/j.ceb.2009.01.002 ↗
3. Environmental sensing through focal adhesions10.1038/nrm2593 ↗
4. Integrins: bidirectional, allosteric signaling machines10.1016/S0092-8674(02)00971-6 ↗
5. Functional atlas of the integrin adhesome10.1038/ncb0807-858 ↗
6. Get a grip: integrins in cell–biomaterial interactions10.1016/j.biomaterials.2005.05.029 ↗
7. Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering10.1038/nbt1055 ↗
8. Designing materials for biology and medicine10.1038/nature02388 ↗
9. Matrix elasticity directs stem cell lineage specification10.1016/j.cell.2006.06.044 ↗
10. Tensional homeostasis and the malignant phenotype10.1016/j.ccr.2005.08.010 ↗
11. Vascular smooth muscle cell durotaxis depends on substrate stiffness gradient strength10.1016/j.bpj.2009.06.021 ↗
12. ECM compliance regulates osteogenesis by influencing MAPK signaling downstream of RhoA and ROCK10.1359/jbmr.081240 ↗
13. Focal adhesion kinase is involved in mechanosensing during fibroblast migration10.1073/pnas.201201198 ↗
14. The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold10.1163/156856298X00325 ↗
15. Synthesis and physicochemical characterization of end-linked poly(ethylene glycol)-co-peptide hydrogels formed by Michael-type addition10.1021/bm025744e ↗
16. Regulating myoblast phenotype through controlled gel stiffness and degradation10.1089/ten.2006.0356 ↗
17. Tunable mechanics of peptide nanofiber gels
18. Polyacrylamide hydrogels for cell mechanics: steps toward optimization and alternative uses10.1016/S0091-679X(07)83002-0 ↗
19. Effects of fibronectin and laminin on structural, mechanical and transport properties of 3D collageneous network10.1007/s10856-006-0686-5 ↗
20. The use of poly(ethylene glycol) hydrogels to investigate the impact of ECM chemistry and mechanics on smooth muscle cells10.1016/j.biomaterials.2006.05.012 ↗
21. Cells on chips10.1038/nature05063 ↗
22. Developing optofluidic technology through the fusion of microfluidics and optics10.1038/nature05060 ↗
23. The origins and the future of microfluidics10.1038/nature05058 ↗
24. A facile method for permanent and functional surface modification of poly(dimethylsiloxane)10.1021/ja071384x ↗
25. Integrin specificity and enhanced cellular activities associated with surfaces presenting a recombinant fibronectin fragment compared to RGD supports10.1016/j.biomaterials.2006.06.027 ↗
26. Surface-initiated atom transfer radical polymerization of oligo(ethylene glycol) methyl methacrylate from a mixed self-assembled monolayer on gold10.1002/adfm.200500426 ↗
27. Micropatterned surfaces with controlled ligand tethering10.1002/jbm.a.32140 ↗
28. Characterization of EGF coupling to aminated silicone rubber surfaces10.1002/bit.21083 ↗
29. Surface tethering of phosphorylcholine groups onto poly(dimethylsiloxane) through swelling–deswelling methods with phospholipids moiety containing ABA-type block copolymers10.1016/j.biomaterials.2007.11.039 ↗
30. A high-density PEG interfacial layer alters the response to an EGF tethered polydimethylsiloxane surface10.1163/156856208786140346 ↗
31. A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands10.1039/C39900001526 ↗