Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance
Source abstract
Abstract
We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein output from small sample sizes, we used bioreactor culture before protein extraction and gel electrophoresis. Mass spectroscopy was used for protein identification. A number of distinct proteins were observed to exhibit significant changes in expression. These changes in protein expression suggest that the cells respond to tailored grooved topographies, with alterations in their proteome concurrent with changes in osteoprogenitor phenotype.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Cellular Mechanics and Interactions · Muscle Physiology and Disorders · Bone health and treatments
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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