Ultrastructural observations and growth of occluding crystals in carious dentine
Source abstract
Abstract
The aim of the present study was to investigate the ultrastructural mechanisms involved in the formation of caries-induced intratubular dentine. Conventional, high resolution and scanning transmission electron microscopy, electron diffraction and energy-dispersive X-ray spectroscopy techniques were used to study the ultrastructure of the inorganic phase in the transparent zone of carious dentine. The results demonstrated that the bulk of the inorganic phase in caries-induced intratubular dentine had an apatite crystal structure with the presence of additional Mg-substituted beta-TCP (beta-tricalcium phosphate) phase in the carious region. Highly oriented apatite crystallites observed in intratubular dentine demonstrated a regulated biomineralization process during the formation of inorganic phase in this region, whereas Mg beta-TCP crystals were presumably formed purely via "dissolution/precipitation" mechanism. The study demonstrated the importance of "dissolution/precipitation" process and the growth kinetics of Mg-substituted beta-TCP crystals in understanding the process of formation of calcium-phosphate crystallites in carious intratubular dentine.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Bone Tissue Engineering Materials · Dental materials and restorations · Endodontics and Root Canal Treatments
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (63)
Show source-provided references
1. Dentin–pulp complex responses to carious lesions10.1159/000092235 ↗
2. A structural analysis of approximal enamel caries lesions and subjacent dentin reactions10.1111/j.1600-0722.1995.tb00006.x ↗
3. The response of deciduous dentine to caries studied by correlated light and electron microscopy10.1159/000259610 ↗
4. Pulp–dentin biology in restorative dentistry. Part 4: Dental caries – characteristics of lesions and pulpal reactions
5. Human dentine sclerosis: a microradiographic survey10.1016/0003-9969(74)90211-8 ↗
6. The ultrastructure and hardness of the transparent layer of human carious dentin10.1177/00220345830620011701 ↗
7. Qualitative and quantitative assessment of intratubular dentin formation in human natural carious lesions10.1007/s002230020023 ↗
8. Crystallographic studies of the role of Mg as a stabilizing impurity in β-Ca3(PO4)2. The crystal structure of pure β-Ca3(PO4)210.1016/0022-4596(74)90030-9 ↗
9. Crystallographic studies of the role of Mg as a stabilizing impurity in β-Ca3(PO4)2. II. Refinement of Mg-containing β-Ca3(PO4)210.1016/0022-4596(77)90002-0 ↗
10. Possible physico-chemical processes in human dentin caries10.1177/00220345870660081401 ↗
11. A transmission electron microscopy study of mineralization in age-induced transparent dentin10.1016/j.biomaterials.2005.05.059 ↗
12. Ultrastructural examination of dentin using focused ion-beam cross-sectioning and transmission electron microscopy10.1016/j.micron.2005.05.011 ↗
13. Minimal intervention dentistry – a review10.1111/j.1875-595X.2000.tb00540.x ↗
14. Two layers of carious dentin: diagnosis and treatment
15. Temperature dependent setting kinetics and mechanical properties of β-TCP–pyrophosphoric acid bone cement10.1039/b507056m ↗
16. Steps in solution growth: dynamics of kinks, bunching and turbulence10.1016/j.jcrysgro.2004.10.094 ↗
17. Odontoblasts: the cells forming and maintaining dentine10.1016/j.biocel.2004.01.006 ↗
18. Dental mineralization
19. Odontoblast function seen as the response of dentinal tissue to dental caries10.1177/08959374010150011701 ↗
20. The extent and distribution of intratubular collagen fibrils in human dentine10.1016/0003-9969(91)90045-V ↗
21. Morphological analysis and chemical content of natural dentin carious lesion zones10.1016/S0940-9602(03)80099-7 ↗
22. Markov · 1995
23. Landau · 1980
24. Landau · 1981
25. Quantitative analysis of the mineral content of sound and carious primary dentine using BSE imaging10.1016/j.archoralbio.2003.08.006 ↗
26. Ultrastructure of dentine carious lesions10.1016/j.archoralbio.2007.08.007 ↗
27. Correlating the mechanical properties to the mineral content of carious dentine—a comparative study using an ultra-micro indentation system (UMIS) and SEM-BSE signals10.1016/j.archoralbio.2003.12.005 ↗
28. International Centre for Diffraction Data. Powder Diffraction File 09-0432; 2005.
29. International Centre for Diffraction Data. Powder Diffraction File 09-0169; 2005.
30. Ultrastructure of dentine carious lesions
31. The dentin substrate: structure and properties related to bonding10.1016/S0300-5712(96)00065-6 ↗
32. Driessens · 1990
33. Chemical characterization of silicon-substituted hydroxyapatite10.1002/(SICI)1097-4636(19990315)44:4<422::AID-JBM8>3.0.CO;2-# ↗
34. Physico-chemical properties and solubility behaviour of multi-substituted hydroxyapatite powders containing silicon
35. In vitro study of remineralization of dentin: effects of ions on mineral induction by decalcified dentin matrix10.1159/000073398 ↗
36. Physico-chemical properties and solubility behaviour of multi-substituted hydroxyapatite powders containing silicon10.1016/j.msec.2006.11.009 ↗
37. Van Rensburg · 1995
38. Noncollagenous proteins and proteoglycans in dentinogenesis
39. Comparative study of in vitro caries-like lesions and natural caries lesions at crown margins10.1111/j.1532-849X.2007.00220.x ↗
40. Transmission electron microscopy of lattice planes in human alveolar bone apatite crystals10.1007/BF02556695 ↗
41. The nature and functional significance of dentin extracellular matrix proteins
42. Dentin matrix protein 1 initiates hydroxyapatite formation in vitro10.1080/713713637 ↗
43. Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 110.1038/nmat945 ↗
44. Phosphorylation of phosphophoryn is crucial for its function as a mediator of biomineralization10.1074/jbc.M500159200 ↗
45. Addadi · 1989
46. Apatite precipitation after incubation of biphasic calcium-phosphate ceramic in various solutions: influence of seed species and proteins10.1002/(SICI)1097-4636(19981215)42:4<530::AID-JBM8>3.0.CO;2-6 ↗
47. The phosphoprotein of the dentin matrix10.1021/bi00860a017 ↗
48. Chemical character of proteins in rat incisors10.1016/0005-2795(72)90266-8 ↗
49. Purification and some properties of the phosphoprotein from rat incisors10.1016/0005-2795(76)90302-0 ↗
50. Mechanism of calcification: role of collagen fibrils and collagen-phosphoprotein complexes in vitro and in vivo10.1002/ar.1092240205 ↗
51. Chemical and crystallographic events in the caries process10.1177/00220345900690S113 ↗
52. Dynamics of the roughening transition10.1103/PhysRevLett.40.733 ↗
53. Statistical properties of steps on crystal surfaces10.1063/1.435833 ↗
54. Formation of crystals in solutions10.1080/00107518908225517 ↗
55. The surface energy of various biomaterials coated with adhesion molecules used in cell culture10.1016/j.colsurfb.2006.11.021 ↗
56. The advance velocity of steps under the influence of volume and surface diffusion, by direct and indirect incorporation of growth units10.1016/0022-0248(82)90027-6 ↗
57. Schlichting · 1968
58. Layer–spiral growth of crystals10.3367/UFNr.0073.196102c.0277 ↗
59. On the dislocation theory of evaporation of crystals10.1080/14786435608238124 ↗
60. Surface tension as a motivation for sintering
61. The use of classical macroscopic concept in surface energy problems
62. Estimation of electrolyte-crystal–aqueous-solution interfacial tension10.1016/0022-0248(83)90443-8 ↗
63. Ion association. VI. Interactions between calcium, magnesium, inorganic phosphate, citrate and protein in normal human plasma10.1172/JCI104306 ↗