Construction of caged polyplexes with a reversible intracellular unpacking property to improve stability and transfection
Source abstract
Abstract
Cross-linking of protein macromonomers accompanies the assembly of viral particles, which provides the virus with high stability in the host. Following inspiration, caged polyplexes were fabricated via a biomimetic cross-linker. Thiolated polyethylenimine was synthesized and showed sufficient DNA condensation ability. Spherical particles with a diameter of about 150nm were formed at an N/P ratio of 10. Shell-cross-linked polyplexes were then constructed by the oxidation of thiol groups in air. All the results indicate that the cross-linking shell via disulfide bonds could improve the stability of polyplexes in the physiological condition and showed a reversible unpacking property at the intracellular GSH concentration. By selecting the proper preparation conditions, polyplexes caged via a biomimetic cross-linker could efficiently release DNA for transfection.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques · Bacteriophages and microbial interactions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (29)
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