Chitosan microparticles incorporating gold as an enhanced contrast flow tracer in dynamic X-ray imaging

In situ monitoring of a biofluid can provide important information on circulatory disorders and a basic understanding on the metabolic mechanisms of living organisms. X-ray imaging has significant advantages as one of the most popular diagnostic tools to seethrough various biological systems. Particle traced velocity field measurement is one of the most popular methods for quantitative analysis of dynamic flow motion. In this study we have developed chitosan microparticles incorporating gold nanoparticles (AuNP) as a new enhanced contrast flow tracer for dynamic X-ray imaging. Gold is a useful material possessing high X-ray absorption ability and also biocompatibility. We chose chitosan as an AuNP delivery system because it can effectively trap AuNPs at high yield. In particular, the unique gold ion reduction ability of and compatibility with surface-modified chitosans are effectively utilized. The physical properties of the Au-chitosan microparticles can be controlled by varying the molecular weight of the chitosan employed and the AuNP incorporation methodology. The environment of the particles and the type of applied X-ray essentially determine the imaging efficiency. The designed chitosan microparticles incorporating Au have been successfully applied to track the digestive mechanisms occurring in delicate insects such as live mosquitoes.