Effect of nanofiber orientation of electrospun nanofibrous scaffolds on cell growth and elastin expression of muscle cells
Source abstract
Abstract
Tissue regeneration after smooth muscle tissue injury is a pivotal issue in tissue engineering. Good artificial scaffolds to continuously form long thin spindle-shaped smooth muscle cells in the damaged muscle tissues are important for tissue regeneration. In this work, poly(lactide-co-glycolide) (PLGA) and poly(ϵ-caprolactone) (PCL) were used to fabricate aligned or random electrospun nanofibrous scaffolds (ENSs) by using electrospinning technique. The cell growth and elastin expression of human vascular smooth muscle cells (HVSMCs) on these membranes were analyzed. Smooth PLGA/PCL film was used as control. The experimental results showed that the aligned ENS could maintain cell shapes of HVSMCs during the culture process. During the HVSMCs proliferation process, elastin expression firstly increase due to cell proliferation, and then decrease due to elastin degradation by elastase secreted by the cells. All these results suggest that aligned PLGA/PCL ENS can be a promising candidate for cell regeneration after smooth muscle tissue injury.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Tissue Engineering and Regenerative Medicine · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (59)
Show source-provided references
1. Regulation and modulation of calcium channels in cardiac, skeletal, and smooth muscle cells10.1152/physrev.1994.74.2.365 ↗
2. The extracellular matrix guides the orientation of the cell division axis10.1038/ncb1307 ↗
3. Extracellular matrix remodelling and cellular differentiation10.1016/S0955-0674(99)00026-5 ↗
4. In vitro reconstructed tissues on hyaluronan-based temporary scaffolding10.1023/A:1008960413362 ↗
5. Tissue engineering on matrix: future of autologous tissue replacement10.1007/s00281-011-0258-8 ↗
6. Electrospun nanofibrous polyurethane membrane as wound dressing10.1002/jbm.b.10058 ↗
7. Biodegradable electrospun fibers for drug delivery10.1016/S0168-3659(03)00372-9 ↗
8. Antibody-functionalized peptidic membranes for neutralization of allogeneic skin10.1016/j.actbio.2014.08.003 ↗
9. Retaining antibodies in tumors with a self-assembling injectable system10.1021/mp300504z ↗
10. The inhibition of tumor growth and metastasis by self-assembled nanofibers of taxol10.1016/j.biomaterials.2012.04.047 ↗
11. Ultralight, soft polymer sponges by self-assembly of short electrospun fibers in colloidal dispersions10.1002/adfm.201500001 ↗
12. Supramolecular peptide vaccines: tuning adaptive immunity10.1016/j.coi.2015.06.007 ↗
13. Electrospun nanofibrous structure: a novel scaffold for tissue engineering10.1002/jbm.10167 ↗
14. A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells10.1016/j.biomaterials.2004.03.005 ↗
15. Aligned biodegradable nanofibrous structure: a potential scaffold for blood vessel engineering10.1016/S0142-9612(03)00593-3 ↗
16. Carbon nanotube networks on different platforms10.1016/j.carbon.2014.07.060 ↗
17. Microporous polymeric 3D scaffolds templated by the layer-by-layer self-assembly10.1002/marc.201400253 ↗
18. Polyurethane/poly(lactic-co-glycolic) acid composite scaffolds fabricated by thermally induced phase separation10.1016/j.biomaterials.2006.12.032 ↗
19. Effect of poly(ethylene glycol) on the properties and foaming behavior of macroporous poly(lactic acid)/sodium chloride scaffold10.1002/app.41181 ↗
20. Fabrication of poly(lactic acid)/graphene cxide foams with highly oriented and elongated cell structure via unidirectional foaming using supercritical carbon dioxide10.1021/ie503434q ↗
21. Thermal, mechanical and thermomechanical properties of tough electrospun poly (imide-co-benzoxazole) nanofiber belts10.1039/C5NJ01040C ↗
22. A review on polymer nanofibers by electrospinning and their applications in nanocomposites10.1016/S0266-3538(03)00178-7 ↗
23. Nanofibers and Electrospinning
24. Strong, light, multifunctional fibers of carbon nanotubes with ultrahigh conductivity10.1126/science.1228061 ↗
25. Increased solubility, liquid-crystalline phase, and selective functionalization of single-walled carbon nanotube polyelectrolyte dispersions10.1021/nn4011544 ↗
26. Macroscopic nanotube fibers spun from single-walled carbon nanotube polyelectrolytes10.1021/nn502552q ↗
27. Carbon nanotubides: an alternative for dispersion, functionalization and composites fabrication10.1039/C5NR03504J ↗
28. Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering10.1016/j.biomaterials.2004.06.051 ↗
29. Two-in-one composite fibers with side-by-side arrangement of silk fibroin and poly(l-lactide) by electrospinning
30. Structure and process relationship of electrospun bioabsorbable nanofiber membranes10.1016/S0032-3861(02)00275-6 ↗
31. Characterization of nano-structured poly(ϵ-caprolactone) nonwoven mats via electrospinning10.1016/S0032-3861(02)00820-0 ↗
32. Incorporation and controlled release of a hydrophilic antibiotic using poly(lactide-co-glycolide)-based electrospun nanofibrous scaffolds10.1016/j.jconrel.2004.04.009 ↗
33. Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds10.1016/j.biomaterials.2006.02.037 ↗
34. Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation10.1016/j.biomaterials.2003.08.042 ↗
35. The effect of gelatin incorporation into electrospun poly(l-lactide-co-ϵ-caprolactone) fibers on mechanical properties and cytocompatibility10.1016/j.biomaterials.2007.12.029 ↗
36. Electrospun chitosan-P(LLA-CL) nanofibers for biomimetic extracellular matrix10.1163/156856208784089661 ↗
37. Fabrication of silk fibroin blended P(LLA-CL) nanofibrous scaffolds for tissue engineering10.1002/jbm.a.32504 ↗
38. Fabrication of PLA/PEG/MWCNT electrospun nanofibrous scaffolds for anticancer drug delivery10.1002/app.41286 ↗
39. In vitro evaluation of electrospun nanofiber scaffolds for vascular graft application10.1002/jbm.a.31287 ↗
40. Fabrication of large pores in electrospun nanofibrous scaffolds for cellular infiltration: a review10.1089/ten.teb.2011.0390 ↗
41. Mesoporous silica-layered biopolymer hybrid nanofibrous scaffold: a novel nanobiomatrix platform for therapeutics delivery and bone regeneration10.1021/acsami.5b00692 ↗
42. Fouling-tolerant nanofibrous polymer membranes for water treatment10.1021/am503874b ↗
43. Preparation and characterization of rana chensinensis skin extract/poly(ϵ-caprolactone) electrospun membranes as antibacterial fibrous mats
44. Unusual and superfast temperature-triggered actuators10.1002/adma.201502133 ↗
45. Nanofiber alignment and direction of mechanical strain affect the ECM production of human ACL fibroblast10.1016/j.biomaterials.2004.04.037 ↗
46. Nanofiber orientation and surface functionalization modulate human mesenchymal stem cell behavior in vitro10.1089/ten.tea.2012.0426 ↗
47. Electrospinning of small diameter 3-D nanofibrous tubular scaffolds with controllable nanofiber orientations for vascular grafts10.1007/s10856-010-4164-8 ↗
48. The effect of poly(l-lactic acid) nanofiber orientation on osteogenic responses of human osteoblast-like MG63 cells10.1016/j.jmbbm.2011.01.008 ↗
49. Grafting of gelatin on electrospun poly (caprolactone) nanofibers to improve endothelial cell spreading and proliferation and to control cell orientation10.1089/ten.2005.11.1149 ↗
50. In vitro cell performance on hydroxyapatite particles/poly(l-lactic acid) nanofibrous scaffolds with an excellent particle along nanofiber orientation10.1016/j.actbio.2011.02.021 ↗
51. Interaction of human smooth muscle cells with nanofibrous scaffolds: effect of fiber orientation on cell adhesion, proliferation, and functional gene expression10.1002/jbm.a.35360 ↗
52. Guiding the orientation of smooth muscle cells on random and aligned polyurethane/collagen nanofibers10.1177/0885328214529002 ↗
53. Poly(d,l-lactide- co-glycolide)/montmorillonite nanoparticles for improved oral delivery of exemestane10.3109/02652048.2012.746749 ↗
54. pH-sensitive nanoparticles of poly(l-histidine)-poly(lactide-co-glycolide)-tocopheryl polyethylene glycol succinate for anti-tumor drug delivery10.1016/j.actbio.2014.09.014 ↗
55. Characterization of neural stem cells on electrospun poly(l-lactic acid) nanofibrous scaffold10.1163/1568562042459733 ↗
56. Novel biodegradable electrospun nanofibrous P (DLLA-CL) balloons for the treatment of vertebral compression fractures10.1016/j.nano.2012.12.003 ↗
57. The differential effects of aligned electrospun PHBHHx fibers on adipogenic and osteogenic potential of MSCs through the regulation of PPARγ signaling10.1016/j.biomaterials.2011.09.089 ↗
58. 3D Artificial bones for bone repair prepared by computed tomography-guided fused deposition modeling for bone repair10.1021/am502716t ↗
59. Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: synthetic ECM analogs for tissue engineering10.1016/S0142-9612(01)00051-5 ↗