PEO-b-PCL grafted niosomes: The cooperativilty of amphiphilic components and their properties in vitro and in vivo

Niosomes belong to drug delivery systems and consist mainly of non-ionic surfactants and cholesterol. In this study, we designed and developed systems composed of non-ionic surfactants i.e. Tween 80, Span 80 and cholesterol with and without poly(ethylene oxide)-b-poly(ε-caprolactone) (PEO-b-PCL) block copolymer, using different molar ratios of the above components. The nanosystems were formed by the thin-film hydration method with purified water as dispersion medium. Several physicochemical techniques were utilized in order to study the physicochemical and morphological characteristics of the prepared assemblies. The results showed that the presence of the block copolymer alters significantly the size and morphology of neat surfactant/cholesterol niosomes. The ageing studies also revealed that the stability is strongly dependent on the nature and the molar ratios of the components. Moreover, neither of the studied nanosystems exhibited elevated signs of cellular toxicity in vitro nor acute systemic toxicity in vivo short-term experiments. This investigation covers a new field of drug delivery platforms those of niosomes composed by different biomaterials i.e. surfactants and block copolymers.