Enhanced synergetic antibacterial activity by a reduce graphene oxide/Ag nanocomposite through the photothermal effect
Source abstract
Abstract
Multidrug-resistant (MDR) bacterial strains have led to notable heathy threats to human beings. The demand for the development of effective antibacterial materials is increasing. Silver nanoparticles (AgNPs) and graphene-based nanomaterials are two major types of nanomaterials that are studied to inhibit and/or kill bacteria. In this study, by combining the excellent photothermal effect of graphene and antibacterial activity of AgNPs, we have applied reduced graphene oxide/silver (RGO/Ag) nanocomposite to destroy the MDR bacteria. The antibacterial activity of the RGO/Ag nanocomposite was systematically investigated using a regular bacterium of Escherichia coli (E. coli) and an MDR bacterium of Klebsiella pneumoniae (Kp). Compared with AgNPs, graphene oxide (GO) and RGO, the RGO/Ag nanocomposite showed significant higher antibacterial efficiency for both regular bacteria and MDR bacteria. Under a near-infrared (NIR) irradiation (0.30 W/cm2 for 10 min), the RGO/Ag nanocomposite demonstrated an enhanced synergetic antibacterial activity through the photothermal effect. Nearly 100 % of E. coli and Kp were killed by the treatment of 15 μg/mL and 30 μg/mL of RGO/Ag nanocomposite, respectively. Moreover, a membrane integrity assay and a reactive oxygen species (ROS) assay demonstrated that the RGO/Ag nanocomposite under NIR irradiation induced the cell membrane disruption and generation of ROS, providing possible mechanisms for their high antibacterial activity besides the photothermal effect. Finally, the cytotoxicity of the RGO/Ag nanocomposites toward different mammalian cells was studied, the cell viabilities retained above 60 % at higher concentrations of RGO/Ag, indicating that the RGO/Ag nanocomposites may be a low cytotoxic, efficient antibacterial agent with the irradiation.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Graphene and Nanomaterials Applications · Nanoplatforms for cancer theranostics · Nanoparticles: synthesis and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (76)
Show source-provided references
1. Antimicrobial resistance and antibiotic stewardship programs in the ICU: insistence and persistence in the fight against resistance. A position statement from ESICM/ESCMID/WAAAR round table on multi-drug resistance10.1007/s00134-017-5036-1 ↗
2. Multidrug-resistant Klebsiella pneumoniae: challenges for treatment, prevention and infection control10.1080/14787210.2018.1522249 ↗
3. Drugs for bad bugs: confronting the challenges of antibacterial discovery10.1038/nrd2201 ↗
4. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study10.1016/S1473-3099(10)70143-2 ↗
5. Iodine-stabilized Cu nanoparticle chitosan composite for antibacterial applications10.1021/am201586w ↗
6. A review on the application of inorganic nano-structured materials in the modification of textiles: focus on anti-microbial properties10.1016/j.colsurfb.2010.03.029 ↗
7. Antimicrobial applications of nanotechnology: methods and literature
8. Facile synthesis of antibacterial chitosan/CuO bio-nanocomposite hydrogel beads
9. Synthesis of β-cyclodextrin-based dendrimer as a novel encapsulation agent10.1002/pi.4637 ↗
10. Antifungal activity and mode of action of silver nano-particles on Candida albicans10.1007/s10534-008-9159-2 ↗
11. Synthesis and antibacterial activity of silver nanoparticles with different sizes10.1007/s11051-008-9428-6 ↗
12. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment10.1007/s11051-010-9900-y ↗
13. Single-walled carbon nanotubes exhibit strong antimicrobial activity10.1021/la701067r ↗
14. Graphene-based photothermal agent for rapid and effective killing of bacteria10.1021/nn304782d ↗
15. Nanostructured hybrid transparent conductive films with antibacterial properties10.1021/nn300852f ↗
16. Antibacterial activity of graphite, graphite oxide, graphene oxide, and reduced graphene oxide: membrane and oxidative stress10.1021/nn202451x ↗
17. Nanotechnology in plant disease management: DNA-directed silver nanoparticles on graphene oxide as an antibacterial against xanthomonas perforans10.1021/nn4034794 ↗
18. Photodynamic antibacterial effect of graphene quantum dots10.1016/j.biomaterials.2014.02.014 ↗
19. Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets10.1038/nnano.2013.125 ↗
20. A single rapid route for the synthesis of reduced graphene oxide with antibacterial activities10.1039/c3ra47573e ↗
21. Characterization of enhanced antibacterial effects of novel silver nanoparticles
22. Antimicrobial effects of silver nanoparticles10.1016/j.nano.2006.12.001 ↗
23. Two dimensional soft material: new faces of graphene oxide10.1021/ar300047s ↗
24. Graphitic design: prospects of graphene-based nanocomposites for solar energy conversion, storage, and sensing10.1021/ar300248f ↗
25. Biomedical applications of graphene and graphene oxide10.1021/ar300159f ↗
26. New horizons for diagnostics and therapeutic applications of graphene and graphene oxide10.1002/adma.201203229 ↗
27. The chemistry of graphene oxide10.1039/B917103G ↗
28. Nano-graphene in biomedicine: theranostic applications10.1039/C2CS35342C ↗
29. Antibacterial mechanisms of graphene-based composite nanomaterials10.1039/C6NR08733G ↗
30. Promises, facts and challenges for graphene in biomedical applications10.1039/C7CS00363C ↗
31. Surface grafting of reduced graphene oxide using nanocrystalline cellulose via click reaction10.1007/s11051-014-2474-3 ↗
32. Magnetic sweet graphene nanosheets: preparation, characterization and application in removal of methylene blue10.1007/s13762-015-0885-z ↗
33. Preparation of efficient magnetic biosorbents by clicking carbohydrates onto graphene oxide10.1007/s10853-015-9082-1 ↗
34. Graphene oxide as an optical biosensing platform: a progress report10.1002/adma.201805043 ↗
35. Discovery of hepatitis C virus NS3 helicase inhibitors by a multiplexed, high-throughput helicase activity assay based on graphene oxide10.1002/anie.201209222 ↗
36. Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy10.1021/ja2010175 ↗
37. Tumor vasculature targeting and imaging in living mice with reduced graphene oxide10.1016/j.biomaterials.2013.01.047 ↗
38. Strong two-photon-induced fluorescence from photostable, biocompatible nitrogen-doped graphene quantum dots for cellular and deep-tissue imaging10.1021/nl400368v ↗
39. Photothermally triggered cytosolic drug delivery via endosome disruption using a functionalized reduced graphene oxide10.1021/nn403096s ↗
40. Gold nanoclusters and graphene nanocomposites for drug delivery and imaging of cancer cells10.1002/anie.201105573 ↗
41. Multifunctional graphene oxide-based triple stimuli-responsive nanotheranostics10.1002/adfm.201400221 ↗
42. Multifunctional mesoporous silica-coated graphene nanosheet used for chemo-photothermal synergistic targeted therapy of glioma10.1021/ja312221g ↗
43. Doxorubicin loaded carboxymethyl cellulose/graphene quantum dot nanocomposite hydrogel films as a potential anticancer drug delivery system10.1016/j.msec.2018.02.010 ↗
44. β-Cyclodextrin grafted magnetic graphene oxide applicable as cancer drug delivery agent: synthesis and characterization10.1016/j.matchemphys.2018.07.022 ↗
45. Quantum-dot-tagged reduced graphene oxide nanocomposites for bright fluorescence bioimaging and photothermal therapy monitored in situ10.1002/adma.201104070 ↗
46. NIR-triggered synergic photo-chemothermal therapy delivered by reduced graphene oxide/carbon/mesoporous silica nanocookies10.1002/adfm.201301763 ↗
47. Using graphene oxide high near-infrared absorbance for photothermal treatment of alzheimer’s disease10.1002/adma.201104864 ↗
48. Development and antibacterial performance of novel polylactic acid-graphene oxide-silver nanoparticle hybrid nanocomposite mats prepared by electrospinning10.1021/acsbiomaterials.6b00766 ↗
49. Enhanced antibacterial activity through the controlled alignment of graphene oxide nanosheets10.1073/pnas.1710996114 ↗
50. Polymers in our daily life10.15171/bi.2017.09 ↗
51. Novel pH sensitive nanocarrier agents based on citric acid dendrimers containing conjugated beta-cyclodextrins
52. Synthesis of magnetic citric-acid-functionalized graphene oxide and its application in the removal of methylene blue from contaminated water10.1002/pi.4769 ↗
53. Sweet graphene I: toward hydrophilic graphene nanosheets via click grafting alkyne-saccharides onto azide-functionalized graphene oxide10.1016/j.carres.2014.06.012 ↗
54. Application of hybrid organic/inorganic dendritic ABA type triblock copolymers as new nanocarriers in drug delivery systems10.1080/00914037.2010.531824 ↗
55. Preparation of gold nanoparticles in the presence of citric acid-based dendrimers containing periphery hydroxyl groups10.1016/j.matchemphys.2011.03.080 ↗
56. Enhanced photothermal effect of plasmonic nanoparticles coated with reduced graphene oxide10.1021/nl4014315 ↗
57. Photothermally enhanced photodynamic therapy delivered by nano-graphene oxide10.1021/nn201560b ↗
58. Synergistic effect of chemo-photothermal therapy using PEGylated graphene oxide10.1016/j.biomaterials.2011.07.071 ↗
59. The preparation of graphene oxide-silver nanocomposites: the effect of silver loads on gram-positive and gram-negative antibacterial activities10.3390/nano8030163 ↗
60. Electron transfer directed antibacterial properties of graphene oxide on metals10.1002/adma.201702149 ↗
61. Graphene oxide–silver nanocomposite as a highly effective antibacterial agent with species-specific mechanisms10.1021/am4005495 ↗
62. A novel wound dressing based on ag/graphene polymer hydrogel: effectively kill bacteria and accelerate wound healing10.1002/adfm.201304202 ↗
63. The use of polyethyleneimine-modified reduced graphene oxide as a substrate for silver nanoparticles to produce a material with lower cytotoxicity and long-term antibacterial activity10.1016/j.carbon.2012.02.002 ↗
64. One-pot synthesis of Ag-iron oxide/reduced graphene oxide nanocomposite via hydrothermal treatment10.1016/j.colsurfa.2014.01.058 ↗
65. Shape selective silver nanostructures decorated amine-functionalized graphene: a promising antibacterial platform10.1016/j.colsurfa.2018.02.031 ↗
66. Stable nanocomposite based on pegylated and silver nanoparticles loaded graphene oxide for long-term antibacterial activity10.1021/acsami.7b03987 ↗
67. Enhanced antibacterial activity of silver-decorated sandwich-like mesoporous silica/reduced graphene oxide nanosheets through photothermal effect
68. One-pot synthesis of reduced graphene oxide/metal (oxide) composites10.1021/acsami.7b12539 ↗
69. The use of a glucose-reduced graphene oxide suspension for photothermal cancer therapy10.1039/c2jm31396k ↗
70. Ultrasmall reduced graphene oxide with high near-infrared absorbance for photothermal therapy10.1021/ja2010175 ↗
71. Enhanced photothermal effect of plasmonic nanoparticles coated with reduced graphene oxide10.1021/nl4014315 ↗
72. Protein-assisted fabrication of nano-reduced graphene oxide for combined in vivo photoacoustic imaging and photothermal therapy10.1016/j.biomaterials.2013.03.090 ↗
73. Antibacterial gold nanoparticle-based photothermal killing of vancomycin-resistant bacteria10.2217/nnm-2017-0380 ↗
74. Au-Ag core-shell nanoparticles for simultaneous bacterial imaging and synergistic antibacterial activity10.1016/j.nano.2016.09.003 ↗
75. Atg7 deficiency intensifies inflammasome activation and pyroptosis in pseudomonas sepsis10.4049/jimmunol.1601196 ↗
76. Decreased levels of miR-224 and the passenger strand of miR-221 increase MBD2, suppressing maspin and promoting colorectal tumor growth and metastasis in mice10.1053/j.gastro.2013.06.008 ↗