Fabrication and characterization of high molecular keratin based nanofibrous membranes for wound healing
Source abstract
Abstract
Keratin is widely used in the biomaterial application, but the keratin prepared by the physical or chemical approach has relatively low molecular weight and mechanical properties. Here we report the preparation of high molecular keratin (HMK) with molecular weight of 120 kDa via multi-enzyme cascade pathway and its application in wound healing. Briefly, we prepared the soluble keratin from wool by keratinase and improved the molecular weight of keratin by transglutaminase (TGase). The HMK was coelectrospun with poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) and the prepared nanofibrous mats demonstrated improved mechanical properties. Ag nanoparticles (AgNPs) were synthesized on the nanofibers via in situ bioreduction, using the above-mentioned keratinase as the reducing agent. It is demonstrated that the PHBV/HMK/AgNPs nanofibrous mats possess favorable antibacterial properties and good biocompatibility. Moreover, in vivo wound healing assessment, the PHBV/HMK/AgNPs membrane displayed better wound healing ability than the control group. These results indicate that PHBV/HMK/AgNPs mats exhibit significant potential in tissue engineering.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Dyeing and Modifying Textile Fibers · Silk-based biomaterials and applications · Skin Protection and Aging
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (45)
Show source-provided references
1. A review of keratin-based biomaterials for biomedical applications10.3390/ma3020999 ↗
2. Keratin films for ocular surface reconstruction: evaluation of biocompatibility in an in-vivo model10.1016/j.biomaterials.2014.11.038 ↗
3. Healing response of rat pulp treated with an injectable keratin hydrogel
4. Preparation and comparative characterization of keratin–chitosan and keratin–gelatin composite scaffolds for tissue engineering applications10.1016/j.msec.2012.02.023 ↗
5. Structural and functional evaluation of oxygenating keratin/silk fibroin scaffold and initial assessment of their potential for urethral tissue engineering10.1016/j.biomaterials.2016.01.032 ↗
6. Recombinant human hair keratin nanoparticles accelerate dermal wound healing10.1021/acsami.9b01725 ↗
7. Synthesis and fabrication of a keratin-conjugated insulin hydrogel for the enhancement of wound healing10.1016/j.colsurfb.2018.12.020 ↗
8. Biological evaluation of human hair keratin scaffolds for skin wound repair and regeneration10.1016/j.msec.2012.10.011 ↗
9. Chitosan and gelatin-based electrospun fibers for bone tissue engineering10.1016/j.ijbiomac.2019.04.115 ↗
10. Microwave-assisted chemical-free hydrolysis of wool keratin10.1177/0040517512452948 ↗
11. An integrated electrodialysis-biocatalysis-spray-drying process for efficient recycling of keratin acid hydrolysis industrial wastewater10.1016/j.cej.2016.05.046 ↗
12. Acoustic cavitation assisted alkaline hydrolysis of wool based keratins to produce organic amendment fertilizers10.1021/acssuschemeng.6b00298 ↗
13. Bacterial keratinases: useful enzymes for bioprocessing agroindustrial wastes and beyond10.1007/s11947-007-0025-y ↗
14. A review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources10.1016/S0960-8524(98)00033-9 ↗
15. Microbial keratinases and their prospective applications: an overview10.1007/s00253-005-0239-8 ↗
16. Mechanical properties and water vapor permeability of edible films made from fractionated soy proteins with ultrafiltration10.1016/j.lwt.2004.03.009 ↗
17. Crosslinking food proteins for improved functionality10.1146/annurev.food.080708.100841 ↗
18. Cross-linking and film-forming properties of transglutaminase-modified collagen fibers tailored by denaturation temperature10.1016/j.foodchem.2018.07.223 ↗
19. Application of transglutaminase to derivatize proteins: 1. Studies on soluble proteins and preliminary results on wool
20. Influence of transglutaminase or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) on the properties of fish-skin gelatin films10.1016/j.foodhyd.2007.07.006 ↗
21. Properties of deamidated gluten films enzymatically cross-linked10.1021/jf0003505 ↗
22. Skin tissue engineering – in vivo and in vitro applications10.1016/j.addr.2011.01.005 ↗
23. Layered nanofiber sponge with an improved capacity for promoting blood coagulation and wound healing10.1016/j.biomaterials.2019.03.008 ↗
24. The exosome – a naturally secreted nanoparticle and its application to wound healing10.1002/adma.201504009 ↗
25. Development of biomaterial scaffold for nerve tissue engineering: biomaterial mediated neural regeneration10.1186/1423-0127-16-108 ↗
26. Biostable electrospun microfibrous scaffolds mitigate hypertrophic scar contraction in an immune-competent murine model10.1016/j.actbio.2015.12.025 ↗
27. Tissue-response and in-vivo degradation of selected polyhydroxyacids - polylactides (Pla), poly(3-hydroxybutyrate) (Phb), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (Phb/Va)10.1002/jbm.820270904 ↗
28. Review – properties of blends and composites based on poly(3-hydroxy)butyrate (PHB) and poly(3-hydroxybutyrate-hydroxyvalerate) (PHBV) copolymers10.1023/A:1004740522751 ↗
29. Development of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fibers for skin tissue engineering: effects of topography, mechanical, and chemical stimuli10.1021/bm200618w ↗
30. Enzymatic assay of hydroxybutyric acid monomer formation in poly(beta-hydroxybutyrate) degradation studies10.1016/0142-9612(95)93263-D ↗
31. Deep infection of cemented total hip arthroplasties caused by coagulase-negative staphylococci10.1302/0301-620X.71B5.2584258 ↗
32. Methicillin-resistant staphylococcus-epidermidis in infection of hip arthroplasties10.1302/0301-620X.76B5.8083259 ↗
33. Results of 2-stage reimplantation for infected total knee arthroplasty10.1016/S0883-5403(98)90071-7 ↗
34. Results of periprosthetic hip and knee infections caused by resistant bacteria10.1097/00003086-200211000-00021 ↗
35. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus10.1002/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO;2-3 ↗
36. Silver bromide nanoparticle/polymer composites: dual action tunable antimicrobial materials10.1021/ja061442z ↗
37. Singh · Bio-nanoparticles: biosynthesis and sustainable biotechnological implications · 201510.1002/9781118677629 ↗
38. Comparative study of antimicrobial activity of AgBr and Ag nanoparticles (NPs)10.1371/journal.pone.0119202 ↗
39. Mining and expression of a metagenome-derived keratinase responsible for biosynthesis of silver nanoparticles10.1021/acsbiomaterials.7b00687 ↗
40. Electrospun polyurethane/keratin/AgNP biocomposite mats for biocompatible and antibacterial wound dressings10.1039/C5TB02358K ↗
41. Chromatographic and electrophoretic investigations of the properties of unprotected low-sulphur wool keratins10.1016/S0021-9673(01)81778-7 ↗
42. In vitro indentation to determine the mechanical properties of epidermis10.1016/j.jbiomech.2011.01.015 ↗
43. Electrospun PHBV nanofibers containing HA and bredigite nanoparticles: fabrication, characterization and evaluation of mechanical properties and bioactivity10.1016/j.compscitech.2015.11.006 ↗
44. In vitro degradation of porous degradable and bioactive PHBV/wollastonite composite scaffolds10.1016/j.polymdegradstab.2004.09.001 ↗
45. Evaluation of the effects of keratin on physical, mechanical and biological properties of poly(3-hydroxybutyrate) electrospun scaffold: potential application in bone tissue engineering10.1016/j.eurpolymj.2020.109502 ↗