An electrostatic self-assembly approach to prepare tebuconazole nanoparticles with improved sustained release and enhanced antifungal activity

Compared with the traditional pesticides, the nanopesticides (NPs) exhibit better sustained release performance, higher utilization efficiency and reduction of environmental pollution. In this study, the antifungal tebuconazole (TEB) loaded nanoparticles (TEB NPs) were prepared by electrostatic self-assembly of the positively charged poly dimethyl diallyl ammonium chloride (PDADMAC) and sulfobutylether-β-cyclodextrin (SCD) inclusion complex (TEB-SCD) with negative charge. The water solubility and thermal stability of TEB were significantly improved after forming the inclusion complex. The blank NPs and TEB NPs were both characterized by particle size, zeta potential, scanning electron microscopy (SEM), sustained release and antifungal activity. The average particle size of the TEB NPs were 411.75 ± 61.65 nm, and the polydispersity index (PDI) showed low values (< 0.3). The TEB NPs were stable for at least 28 days at 25 °C. Compared with pure TEB, TEB NPs showed the sustained release properties. In addition, TEB NPs exhibited better antifungal activity than TEB industrial concentrate (TC, 98%) and commercially available TEB suspension concentrate (SC) with the 96.33 ± 13.52% antifungal rate of fusarium graminearum. The results indicated that the TEB NPs can improve the antifungal activity and reduce environmental pollution.