Interface-driven Sr-morin complexation at Langmuir monolayers for bioactive coating design
Source abstract
Abstract
Flavonoid-metal complexes are widely studied because of their interesting luminescent behavior and biological activity. Despite the extensive exploration of flavonoid-metal coordination processes in solution, the formation of complexes using the flavonoid molecule inserted in a lipid membrane has been little investigated. This effect could provide important insight into the biological activity of flavonoids at lipid membranes and could represent an attractive strategy to design supramolecular structures. Here, we studied the complexation between Sr2+ and morin inserted in an octadecylphosphonic acid (OPA) Langmuir monolayer. This is a relevant system due to the synergism imposed by the association of the Sr2+ ability to control bone formation/resorption with the morin antioxidative effect. Morin incorporation into the OPA monolayers and further Sr2+ complexation were monitored by surface pressure isotherms. Electronic absorption spectroscopy and fluorescence techniques showed Sr-morin complexation both in solution and at the air-liquid interface. Although morin complexation has been described to occur only at basic pH, the specific thermodynamic properties at the air-liquid interface drove metal complexation. LB films were deposited on Ti surfaces, and the resulting OPA/Sr-morin coatings exhibited high surface free energy and increase on its polar component. This optimized surface feature supported further serum protein adsorption and osteoblast growth and differentiation, indicating that these lipid-based coatings are promising for bioactive coating design. This study paves the way for the use of this lipid-based coating in the design of implants for faster osteointegration. Moreover, flavonoid-metal complexation at membranes could also help to shed light on the biological role played by flavonoids.
Source: europepmc · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Force Microscopy Techniques and Applications · Computational Drug Discovery Methods · thermodynamics and calorimetric analyses
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (63)
Show source-provided references
1. Properties and applications of flavonoid metal complexes10.1039/C5RA05069C ↗
2. Flavonoids: an overview10.1017/jns.2016.41 ↗
3. Lipid oxidation and improving the oxidative stability10.1039/b922183m ↗
4. Flavonoids as antioxidants10.1021/np9904509 ↗
5. Iron chelation by the powerful antioxidant flavonoid quercetin10.1021/jf060986h ↗
6. Iron-binding properties of plant phenolics and cranberry’s bio-effects
7. Synthesis, spectral and electrochemical properties of Al(III) and Zn(II) complexes with flavonoids10.1016/j.saa.2004.07.029 ↗
8. Synthesis, characterization, spectroscopic and antioxidation studies of Cu(II)–morin complex10.1016/j.molstruc.2009.12.037 ↗
9. Oxidative DNA cleavage induced by an iron(III) flavonoid complex: synthesis, crystal structure and characterization of chlorobis(flavonolato)(methanol) iron(III) complex10.1016/j.jinorgbio.2006.01.036 ↗
10. New complexes of La(III), Ce(III), Pr(III), Nd(III), Sm(III), Eu(III) and Gd(III) ions with morin10.1016/j.jinorgbio.2007.01.005 ↗
11. Synthesis and evaluation of antioxidant and antibacterial properties of morin complexes10.1080/00958972.2011.590192 ↗
12. The effects of strontium on bone mineral: a review on current knowledge and microanalytical approaches10.1016/j.micron.2015.10.006 ↗
13. Dual effect of strontium ranelate: stimulation of osteoblast differentiation and inhibition of osteoclast formation and resorption in vitro10.1016/j.bone.2007.08.043 ↗
14. Flavonoid quercetin decreases osteoclastic differentiation induced by RANKL via a mechanism involving NF k B and AP-110.1002/jcb.20071 ↗
15. The flavonoid fisetin promotes osteoblasts differentiation through Runx2 transcriptional activity10.1002/mnfr.201300836 ↗
16. Synthesis of Sr–morin complex and its in vitro response: decrease in osteoclast differentiation while sustaining osteoblast mineralization ability10.1039/C8TB02045K ↗
17. Sr 2+ -substituted CaCO 3 nanorods: impact on the structure and bioactivity10.1021/acs.cgd.8b00017 ↗
18. Lipid-mediated growth of SrCO3/CaCO3 hybrid films as bioactive coatings for Ti surfaces10.1016/j.msec.2019.02.023 ↗
19. Accelerated bone ingrowth by local delivery of strontium from surface functionalized titanium implants10.1016/j.biomaterials.2013.04.031 ↗
20. The flavonoid quercetin inhibits titanium dioxide (TiO2)-induced chronic arthritis in mice10.1016/j.jnutbio.2017.10.010 ↗
21. Improvement in film preparation and fluorescence decay measurements of Ru(bpy) derivative for determination of photoinduced electron transfer rates in heterogeneous Langmuir-Blodgett films10.1016/0040-6090(92)90229-5 ↗
22. Aluminum ion complex formation with 3-hydroxyflavone in Langmuir and Langmuir - Blodgett films10.1016/S0927-7757(01)00963-3 ↗
23. Formation of thin luminescent Eu 3 þ -LB films by in situ coordination with Langmuir monolayers10.1016/j.jlumin.2011.12.081 ↗
24. Photoaccelerated complex formation of a novel Langmuir−Blodgett film with transition metal ions and its ion selectivity10.1021/jp962836n ↗
25. The formation and stability of self-assembled monolayers of octadecylphosphonic acid on titania10.1016/j.colsurfa.2005.12.057 ↗
26. Infrared spectra of alkylphosphonic acid bound to aluminium surfaces10.1002/masy.200750837 ↗
27. Surface modification of titanium with phosphonic acid to improve bone bonding: characterization by XPS and ToF-SIMS10.1021/la010908i ↗
28. Biomimetic collagen/phospholipid coatings improve formation of hydroxyapatite nanoparticles on titanium10.1016/j.msec.2017.03.204 ↗
29. Formation of carbonated hydroxyapatite films on metallic surfaces using dihexadecyl phosphate-LB film as template10.1016/j.colsurfb.2014.03.029 ↗
30. Different compact hybrid Langmuir-Blodgett-film coatings modify biomineralization and the ability of osteoblasts to grow10.1002/jbm.b.34069 ↗
31. Optical absorption and fluorescence spectroscopy studies of Artepillin C, the major component of green propolis10.1016/j.saa.2018.02.059 ↗
32. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays10.1016/0022-1759(83)90303-4 ↗
33. Estrogen and phenol red free medium for osteoblast culture: study of the mineralization ability10.1007/s10616-015-9844-2 ↗
34. Confocal laser scanning microscopy in orthopaedic research10.1016/j.proghi.2005.02.001 ↗
35. Bio-inspired synthesis of hybrid tube-like structures based on CaCO 3 and type I-collagen10.1039/C6RA18984A ↗
36. Size effect of hydroxyapatite nanoparticles on proliferation and apoptosis of osteoblast-like cells10.1016/j.actbio.2008.07.023 ↗
37. Estimation of the surface free energy of polymers10.1002/app.1969.070130815 ↗
38. Dispersion-polar surface tension properties of organic solids10.1080/0021846708544582 ↗
39. Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions10.2298/JSC0710921M ↗
40. Comparative spectroscopic and mechanistic study of chelation properties of fisetin with iron in aqueous buffered solutions. Implications on in vitro antioxidant activity10.1039/c0dt01834a ↗
41. Determination of dissociation constants of flavonoids by capillary electrophoresis10.1002/elps.200410258 ↗
42. Fluorescence behavior of (selected) flavonols: a combined experimental and computational study10.1039/c3cp44267e ↗
43. Dependence of flavonol functions on their chemical structure in chloroplast energy reactions
44. Synthesis of Sr-morin complex and its: in vitro response: decrease in osteoclast differentiation while sustaining osteoblast mineralization ability10.1039/C8TB02045K ↗
45. Excited state proton-transfer spectroscopy of 3-Hydroxyflavone and quercetin10.1016/0009-2614(79)87221-8 ↗
46. Effect of reverse micelles on the intramolecular excited state proton transfer (ESPT) and dual luminescence behaviour of 3-hydroxyflavone10.1016/0584-8539(95)01622-8 ↗
47. Time resolved fluorescence spectroscopy of quercetin and morin complexes with Al3+10.1016/S1386-1425(01)00515-7 ↗
48. Ultrathin films of lipids to investigate the action of a flavonoid with cell membrane models10.1016/j.msec.2014.11.061 ↗
49. Progress in characterization of Langmuir monolayers by consideration of compressibility10.1016/j.cis.2006.11.006 ↗
50. Determination of pKa at the air/water interface by second harmonic generation10.1016/0009-2614(90)85263-C ↗
51. Probing the proton excess at interfaces by second harmonic generation10.1016/0009-2614(89)85186-3 ↗
52. Luminescent Langmuir–Blodgett film of a new amphiphilic Eu3+ β-diketonate10.1016/j.jlumin.2008.01.002 ↗
53. In situ complex formation of long-chain Schiff bases with silver(I) ion in monolayers and their Langmuir-Blodgett films10.1021/la0010121 ↗
54. Osseointegrated titanium implants: requirements for ensuring a long-lasting, direct bone-to-implant anchorage in man10.3109/17453678108991776 ↗
55. Titanium coatings and surface modifications: toward clinically useful bioactive implants10.1021/acsbiomaterials.6b00604 ↗
56. Understanding and controlling the bone-implant interface10.1016/S0142-9612(99)00160-X ↗
57. The role played by modified bioinspired surfaces in interfacial properties of biomaterials10.1007/s12551-017-0306-2 ↗
58. Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers10.1016/j.biomaterials.2007.03.013 ↗
59. The role of surface free energy in osteoblast–biomaterial interactions10.1179/1743280414Y.0000000038 ↗
60. Integrin expression and osteopontin regulation in human fetal osteoblastic cells mediated by substratum surface characteristics10.1089/ten.2005.11.19 ↗
61. Collagen-supported CaCO3 cylindrical particles enhance Ti bioactivity10.1016/j.surfcoat.2018.11.071 ↗
62. Osteoblast differentiation at a glance10.12659/MSMBR.901142 ↗
63. Lipid-mediated growth of SrCO3/CaCO3 hybrid films as bioactive coatings for Ti surfaces10.1016/j.msec.2019.02.023 ↗