Electrospun linear polyethyleneimine scaffolds for cell growth

Unique biocompatible scaffolds were produced by electrospinning cross-linked linear polyethyleneimine (PEI) with succinic anhydride and 1,4-butanediol diglycidyl ether. Nonwoven mats of PEI fibers in the range of 1600-687nm were evaluated as interaction scaffolds for normal human fibroblast (NHF) cells. The electrospun scaffolds were characterized by Fourier transform infrared spectroscopy and ultraviolet-visible spectroscopy. The growth of the NHF cells was followed by scanning electron microscopy as well as optical and fluorescence microscopies. Cell viability was evaluated by staining with propidium iodide for dead cells and fluorescein diacetate for live cells. Immunofluorescence with fixed cells on the scaffolds was examined by staining the endoplasmic reticulum with rabbit anti-GRP 78/Alexa 488 goat anti-rabbit and staining the nuclei with 4'-6'-diamidino-2-phenylindole. Fluorescence studies confirmed that NHF cells attached and spread throughout the cross-linked linear polyethyleneimine scaffold. The attachment and spreading of cells suggests that electrospun linear polyethyleneimine scaffolds support growth of normal human fibroblasts cells. Thus, these biomaterial scaffolds may be useful in tissue engineering.