DataVXSmallPaper
journal article·2024-05Open access

Single‐Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications

Sergi Brosel‐Oliu, Gemma Rius, Anna Aviñó, Nako Nakatsuka, Xavi Illa, Elena del Corro, Marta Delgà‐Fernández, Eduard Masvidal‐Codina, Natalia Rodríguez, Juan Pedro Merino, Alejandro Criado, Maurizio Prato, Raphaela Tkatchenko, Ramón Eritja, Philippe Godignon, José Antonio Garrido, Rosa Villa, Anton Guimerà, Elisabet Prats‐Alfonso

Small· Volume 20· Issue 18
Publisher pageOpen full text
Source abstract

Abstract

Abstract Graphene solution‐gated field‐effect transistors (gSGFETs) offer high potential for chemical and biochemical sensing applications. Among the current trends to improve this technology, the functionalization processes are gaining relevance for its crucial impact on biosensing performance. Previous efforts are focused on simplifying the attachment procedure from standard multi‐step to single‐step strategies, but they still suffer from overreaction, and impurity issues and are limited to a particular ligand. Herein, a novel strategy for single‐step immobilization of chemically modified aptamers with fluorenylmethyl and acridine moieties, based on a straightforward synthetic route to overcome the aforementioned limitations is presented. This approach is benchmarked versus a standard multi‐step strategy using thrombin as detection model. In order to assess the reliability of the functionalization strategies 48‐gSGFETs arrays are employed to acquire large datasets with multiple replicas. Graphene surface characterization demonstrates robust and higher efficiency in the chemical coupling of the aptamers with the single‐step strategy, while the electrical response evaluation validates the sensing capability, allowing to implement different alternatives for data analysis and reduce the sensing variability. In this work, a new tool capable of overcome the functionalization challenges of graphene surfaces is provided, paving the way toward the standardization of gSGFETs for biosensing purposes.
Source: crossref · Quality: baseline

Research context

NanomaterialsElectronic materials

OpenAlex topics: Graphene research and applications · Molecular Junctions and Nanostructures · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Instituto de Microelectrónica de Barcelona IMB‐CNM (CSIC) Campus UAB Bellaterra 08193 Barcelona Spain

Institute for Advanced Chemistry of Catalonia (IQAC‐CSIC) Jordi Girona 18–26 Barcelona 08034 Spain

Centro de Investigación Biomédica en Red de Bioingeniería Biomateriales y Nanomedicina Instituto de Salud Carlos III Madrid 28029 Spain

Laboratory of Biosensors and Bioelectronics Institute for Biomedical Engineering, ETH Zürich Zürich 8092 Switzerland

Catalan Institute of Nanoscience and Nanotechnology (ICN2) CSIC and BIST Campus UAB Bellaterra 08193 Barcelona Spain

Center for Cooperative Research in Biomaterials (CIC biomaGUNE) Basque Research and Technology Alliance (BRTA) Paseo de Miramon 194 Donostia‐San Sebastián 20014 Spain

CICA‐Centro Interdisciplinar de Química e Bioloxía Rúa as Carballeiras Universidade da Coruña A Coruña 15071 Spain

Ikerbasque Basque Foundation for Science Bilbao 48013 Spain

Department of Chemical and Pharmaceutical Sciences University of Trieste Via L. Giorgieri 1 Trieste 3412 7 Italy

ICREA Pg. Lluís Companys 23 Barcelona 08010 Spain

Funding

Generalitat de Catalunya · Ministerio de Ciencia e Innovación · Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina · Instituto de Salud Carlos III · Consejo Superior de Investigaciones Científicas

References (74)

Show source-provided references
1. 10.1088/0957-4484/24/35/35550210.1088/0957-4484/24/35/355502 ↗
2. 10.1016/B978-0-12-813349-1.00006-810.1016/B978-0-12-813349-1.00006-8 ↗
3. 10.3390/diagnostics703004510.3390/diagnostics7030045 ↗
4. 10.1016/j.sna.2017.12.02810.1016/j.sna.2017.12.028 ↗
5. 10.1098/rsfs.2016.013210.1098/rsfs.2016.0132 ↗
6. 10.1016/j.bioelechem.2020.10762010.1016/j.bioelechem.2020.107620 ↗
7. 10.1007/s00216-014-7804-x10.1007/s00216-014-7804-x ↗
8. 10.1016/j.bios.2020.11247110.1016/j.bios.2020.112471 ↗
9. 10.1021/acsami.7b0706310.1021/acsami.7b07063 ↗
10. 10.1039/C8RA09577A10.1039/C8RA09577A ↗
11. 10.1002/adfm.20170397610.1002/adfm.201703976 ↗
12. 10.1088/1361-6463/aadcca10.1088/1361-6463/aadcca ↗
13. 10.1002/adfm.20100072410.1002/adfm.201000724 ↗
14. 10.1016/j.sse.2022.10825910.1016/j.sse.2022.108259 ↗
15. 10.1039/D2TC01631A10.1039/D2TC01631A ↗
16. A.Guimerà‐Brunet N.Schaefer A.Barbero P.Godignon G.Rius E.Del Corro J.Bousquet C.Hébert R. C.Wykes M. V.Sanchez‐Vives R.Villa E.Masvidal‐Codina J. A.Garrido X.Illa M.Dasilva A.Bonaccini‐Calia E.Prats‐Alfonso J.Martínez‐Aguilar J. M.De la Cruz R.Garcia‐Cortadella at 2019 IEEE Int. Electron Devices Meeting (IEDM) IEEE Piscataway NJ USA2019.
17. 10.1063/5.005859110.1063/5.0058591 ↗
18. 10.1186/s12951-023-01898-z10.1186/s12951-023-01898-z ↗
19. 10.1021/acs.analchem.2c0339910.1021/acs.analchem.2c03399 ↗
20. 10.1039/D0AN01661F10.1039/D0AN01661F ↗
21. Szunerits S. · Anal. Bioanal. Chem. · 2023 · 1
22. 10.1016/j.carbon.2020.01.06610.1016/j.carbon.2020.01.066 ↗
23. 10.1038/s41563-018-0249-410.1038/s41563-018-0249-4 ↗
24. 10.1038/s41565-021-01041-910.1038/s41565-021-01041-9 ↗
25. 10.1088/2053-1583/ab797610.1088/2053-1583/ab7976 ↗
26. 10.1038/s41467-020-20546-w10.1038/s41467-020-20546-w ↗
27. 10.1002/smll.20190282010.1002/smll.201902820 ↗
28. 10.1021/cr300041210.1021/cr3000412 ↗
29. 10.1021/acs.chemrev.5b0062010.1021/acs.chemrev.5b00620 ↗
30. 10.1021/jz201273r10.1021/jz201273r ↗
31. 10.1002/smll.20120219610.1002/smll.201202196 ↗
32. 10.1039/b920539j10.1039/b920539j ↗
33. 10.1038/ncomms1490210.1038/ncomms14902 ↗
34. 10.1021/acsami.5b0394110.1021/acsami.5b03941 ↗
35. 10.1073/pnas.160375311310.1073/pnas.1603753113 ↗
36. 10.1126/science.aao675010.1126/science.aao6750 ↗
37. 10.1002/smll.20140307310.1002/smll.201403073 ↗
38. 10.29245/2578-2967/2020/3.118610.29245/2578-2967/2020/3.1186 ↗
39. 10.1016/j.aca.2014.10.02310.1016/j.aca.2014.10.023 ↗
40. 10.3390/molecules2503068010.3390/molecules25030680 ↗
41. 10.1021/acs.langmuir.6b0423710.1021/acs.langmuir.6b04237 ↗
42. 10.1021/acsanm.8b0042010.1021/acsanm.8b00420 ↗
43. 10.1002/adhm.20170073610.1002/adhm.201700736 ↗
44. 10.1016/j.apsusc.2020.14540910.1016/j.apsusc.2020.145409 ↗
45. 10.3390/s2104133510.3390/s21041335 ↗
46. 10.1016/j.bmcl.2008.02.07410.1016/j.bmcl.2008.02.074 ↗
47. 10.1016/j.bmc.2010.09.02310.1016/j.bmc.2010.09.023 ↗
48. 10.1016/j.snb.2013.10.06110.1016/j.snb.2013.10.061 ↗
49. 10.1039/D1SD00036E10.1039/D1SD00036E ↗
50. 10.1021/acsomega.8b0086610.1021/acsomega.8b00866 ↗
51. 10.1103/PhysRevB.96.11540110.1103/PhysRevB.96.115401 ↗
52. 10.1021/acs.analchem.5b0173610.1021/acs.analchem.5b01736 ↗
53. 10.1021/acs.analchem.0c0503810.1021/acs.analchem.0c05038 ↗
54. 10.1021/ac201778h10.1021/ac201778h ↗
55. 10.1021/la025911w10.1021/la025911w ↗
56. 10.1126/sciadv.170124710.1126/sciadv.1701247 ↗
57. 10.1016/S0021-9258(18)89113-X10.1016/S0021-9258(18)89113-X ↗
58. 10.1021/bi201651910.1021/bi2016519 ↗
59. 10.1039/c3nr00511a10.1039/c3nr00511a ↗
60. 10.1038/s41598-019-50412-910.1038/s41598-019-50412-9 ↗
61. 10.1016/j.aca.2014.04.05510.1016/j.aca.2014.04.055 ↗
62. 10.1016/j.transci.2007.12.00510.1016/j.transci.2007.12.005 ↗
63. 10.1016/j.tibtech.2021.01.00510.1016/j.tibtech.2021.01.005 ↗
64. 10.3389/fchem.2019.0033910.3389/fchem.2019.00339 ↗
65. 10.1016/j.carbon.2019.06.08110.1016/j.carbon.2019.06.081 ↗
66. 10.1038/s41467-020-19053-910.1038/s41467-020-19053-9 ↗
67. 10.1038/s41467-020-16979-y10.1038/s41467-020-16979-y ↗
68. 10.1016/j.bios.2021.11389010.1016/j.bios.2021.113890 ↗
69. 10.1021/acs.analchem.1c0312910.1021/acs.analchem.1c03129 ↗
70. 10.1039/D0AN00251H10.1039/D0AN00251H ↗
71. 10.1002/adhm.20210073410.1002/adhm.202100734 ↗
72. 10.1016/j.bios.2021.11360510.1016/j.bios.2021.113605 ↗
73. 10.1063/1.477558310.1063/1.4775583 ↗
74. 10.1038/nmeth.281310.1038/nmeth.2813 ↗