journal article·2021-07-12

Fabrication of 2D and 3D Cell Cluster Arrays Using a Cell-Friendly Photoresist

Jeehun Park, Seong-Eun Kim, Yongbum Cho, Seongmin An, Dowon Moon, Inae Park, Junsang Doh

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Abstract

Cells in 3D behave differently than cells in 2D. We develop a new method for the fabrication of 2D and 3D cell cluster arrays on an identical substrate using a cell-friendly photoresist, which enables comparative study between cells in 2D and 3D cell clusters. The fabricated cell cluster arrays maintain their structure up to 3 days with good viability. Using this method, 2D and 3D cancer cell clusters with comparable sizes are fabricated, and natural killer (NK) cell cytotoxicity assays are performed to assess how dimensionality of cancer cell clusters influence their susceptibility to immune cell-mediated killing.
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Research context

OpenAlex topics: CAR-T cell therapy research · 3D Printing in Biomedical Research · Microfluidic and Bio-sensing Technologies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Research Institute of Advanced Materials (RIAM), Seoul National University, 1, Gwanak-ro, Seoul 08826, South Korea

Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77, Cheongam-ro, Pohang, Gyeongbuk 37673, South Korea

School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), 77, Cheongam-ro, Pohang, Gyeongbuk 37673, South Korea

Department of Materials Science and Engineering, Institute of Engineering Research, BioMAX, Seoul National University, 1, Gwanak-ro, Seoul 08826, South Korea

Funding

National Research Foundation of Korea · Seoul National University

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