Biomimetic Extracellular Scaffolds by Microfluidic Superstructuring of Nanofibers

Jack Kolberg-Edelbrock; Thomas J. Cotey; Steven Y. Ma; Litsa M. Kapsalis; Delaney M. Bondoc; Sieun Ruth Lee; Hiroaki Sai; Cara S. Smith; Feng Chen; Alexandra N. Kolberg-Edelbrock; Madison E. Strong; Samuel I. Stupp

doi:10.1021/acsbiomaterials.2c01098

journal article·2023-03-13

Biomimetic Extracellular Scaffolds by Microfluidic Superstructuring of Nanofibers

Jack Kolberg-Edelbrock, Thomas J. Cotey, Steven Y. Ma, Litsa M. Kapsalis, Delaney M. Bondoc, Sieun Ruth Lee, Hiroaki Sai, Cara S. Smith, Feng Chen, Alexandra N. Kolberg-Edelbrock, Madison E. Strong, Samuel I. Stupp

ACS Biomaterials Science & Engineering· Volume 9· Issue 3

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Source abstract

Abstract

The extracellular matrix is a dynamic framework bearing chemical and morphological cues that support many cellular functions, and artificial analogs with well-defined chemistry are of great interest for biomedical applications. Herein, we describe hierarchical, extracellular-matrix-mimetic microgels, termed "superbundles" (SBs) composed of peptide amphiphile (PA) supramolecular nanofiber networks created using flow-focusing microfluidic devices. We explore the effects of altered flow rate ratio and PA concentration on the ability to create SBs and develop design rules for producing SBs with both cationic and anionic PA nanofibers and gelators. We demonstrate the morphological similarities of SBs to decellularized extracellular matrices and showcase their ability to encapsulate and retain proteinaceous cargos with a wide variety of isoelectric points. Finally, we demonstrate that the novel SB morphology does not affect the well-established biocompatibility of PA gels.

Source: openalex_inverted_index · Quality: baseline

Research context

Nanomaterials Biomaterials

OpenAlex topics: Supramolecular Self-Assembly in Materials · Polydiacetylene-based materials and applications · Luminescence and Fluorescent Materials

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Materials Science and Engineering, McCormick School of Engineering, Northwestern University, 2220 Campus Drive, Room 2036, Evanston, Illinois 60208-0893, United States

Medical Scientist Training Program, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Morton 1-670, Chicago, Illinois 60611-3008, United States

Department of Chemistry, Weinberg College of Arts and Sciences, Northwestern University, 2145 Sheridan Road, Tech K148, Evanston, Illinois 60208-0834, United States

Simpson Querrey Institute for BioNanotechnology, Northwestern University, 303 East Superior Street, Lurie 11, Chicago, Illinois 60611-3015, United States

Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University, 2145 Sheridan Road, Tech E310, Evanston, Illinois 60208-0893, United States

Department of Medicine, Feinberg School of Medicine, Northwestern University, 676 North Saint Clair Street, Arkes Suite 2330, Chicago, Illinois 60611-2915, United States

Funding

Basic Energy Sciences · Division of Graduate Education

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