DataVXACS Biomaterials Science & EngineeringPaper
journal article·2016-09-12

Increase in Dye:Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells

Sriram Vaidyanathan, Milan Kaushik, Casey Dougherty, Rahul Rattan, Sascha N. Goonewardena, Mark M. Banaszak Holl, Janet Monano, Stassi DiMaggio

ACS Biomaterials Science & Engineering· Volume 2· Issue 9
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Source abstract

Abstract

Neutral generation 3 poly(amidoamine) dendrimers were labeled with Oregon Green 488 (G3-OG n ) to obtain materials with controlled fluorophore:dendrimer ratios (n = 1–2), a mixture containing mostly 3 dyes per dendrimer, a mixture containing primarily 4 or more dyes per dendrimer ( n = 4+), and a stochastic mixture ( n = 4 avg ). The UV absorbance of the dye conjugates increased linearly as n increased and the fluorescence emission decreased linearly as n increased. Cellular uptake was studied in RAW cells and HEK 293A cells as a function of the fluorophore:dendrimer ratio (n). The cellular uptake of G3-OG n ( n = 3, 4+, 4 avg ) into RAW cells was significantly lower than G3-OG n ( n = 1, 2). The uptake of G3-OG n ( n = 3, 4+, 4 avg ) into HEK 293A cells was not significantly different from G3-OG 1 . Thus, the fluorophore:dendrimer ratio was observed to change the extent of uptake in the macrophage uptake mechanism but not in the HEK 293A cell. This difference in endocytosis indicates the presence of a pathway in the macrophage that is sensitive to hydrophobicity of the particle.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Dendrimers and Hyperbranched Polymers · RNA Interference and Gene Delivery · Advanced biosensing and bioanalysis techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biomedical Engineering, University of Michigan, 1107 Carl A. Gerstacker Building, 2200 Bonisteel Boulevard, Ann Arbor, Michigan 48109, United States

Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, United States

Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, 9220 MSRB III, 1150 West Medical Center Drive, SPC 5648, Ann Arbor, Michigan 48109, United States

Division of Cardiovascular Medicine, Internal Medicine, University of Michigan, 1500 E Medical Center Drive, Ann Arbor, Michigan 48109, United States

Veterans Affairs Health System, 2215 Fuller Road, Ann Arbor, Michigan 48105, United States

Macromolecular Science and Engineering, University of Michigan, 3062C H.H. Dow Building, 2300 Hayward Street, Ann Arbor, Michigan 48109, United States

Department of Chemistry, Xavier University of Louisiana, 1 Drexel Drive, New Orleans, Louisiana 70125, United States

Funding

National Heart, Lung, and Blood Institute · National Institute on Minority Health and Health Disparities · National Institute of General Medical Sciences · National Science Foundation · Louisiana Cancer Research Consortium

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