journal article·2020-03-09Open access

Growth and Spatial Control of Murine Neural Stem Cells on Reflectin Films

Rylan Kautz, Long Phan, Janahan Arulmoli, Atrouli Chatterjee, Justin P. Kerr, Mahan Naeim, James Long, Alex Allevato, Jessica E. Leal-Cruz, LeAnn Le, Parsa Derakhshan, Francesco Tombola, Lisa A. Flanagan, Alon A. Gorodetsky

Abstract

Stem cells have attracted significant attention due to their regenerative capabilities and their potential for the treatment of disease. Consequently, significant research effort has focused on the development of protein- and polypeptide-based materials as stem cell substrates and scaffolds. Here, we explore the ability of reflectin, a cephalopod structural protein, to support the growth of murine neural stem/progenitor cells (mNSPCs). We observe that the binding, growth, and differentiation of mNSPCs on reflectin films is comparable to that on more established protein-based materials. Moreover, we find that heparin selectively inhibits the adhesion of mNSPCs on reflectin, affording spatial control of cell growth and leading to a >30-fold change in cell density on patterned substrates. The described findings highlight the potential utility of reflectin as a stem cell culture material.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Silk-based biomaterials and applications · Tissue Engineering and Regenerative Medicine · 3D Printing in Biomedical Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemical Engineering and Materials Science, University of California, Irvine, 916 Engineering Tower, Irvine, California 92697, United States

Department of Biomedical Engineering, University of California, Irvine, 3120 Natural Sciences II, Irvine, California 92697, United States

Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, 845 Health Sciences Road, Irvine, California 92697, United States

Department of Mechanical and Aerospace Engineering, University of California, Irvine, 4200 Engineering Gateway Building, Irvine, California 92697, United States

Department of Physiology and Biophysics, University of California, Irvine, 825 Health Sciences Road, Irvine, California 92697, United States

Department of Neurology, University of California, Irvine, 200 South Manchester Avenue, Orange, California 92868, United States

Department of Chemistry, University of California, Irvine, 1102 Natural Sciences II, Irvine, California 92697, United States

Funding

Division of Integrative Organismal Systems · Division of Graduate Education · Air Force Office of Scientific Research · National Institute of General Medical Sciences

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