journal article·2025-01-13

Repair of Cartilage Defects Using ATDC5 Cells Treated with BBR Loaded in Chitosan Hydrogel

Yixiao Chen, Guoqing Li, Yufeng Ge, Su Liu, Jian Weng, Jianjing Lin, Ao Xiong, Hui Zeng, Xinbao Wu, Jun Yang, Fei Yu

Publisher page

Abstract

In this study, we explore the cartilage defect repair mechanism by phosphocreatine-grafted chitosan hydrogels loaded with berberine-treated ATDC5 cells (CSMP@BBR@ATDC5). Under the optimal concentrations of LPS and BBR ideal conditions, ATDC5 cell toxicity and proliferation were detected with AM/PI and EdU staining. Additionally, qPCR and Western blot were employed to detect the expression of the SIRT1/BMP4 signaling pathway and chondrogenic-related factors in ATDC5 cells. Moreover, BBR-treated ATDC5 was seeded into a phosphocreatine-grafted chitosan hydrogel system. Subsequently, the cartilage defect was established in mice. After 4, 8, and 12 weeks, knee specimens were collected to evaluate the repair of cartilage defects. According to our findings, BBR can increase ATDC5 viability by LPS treatment. Likewise, it upregulates the SIRT1/BMP4 signaling pathway expression and chondrogenic-related factors. Another, it was shown by histological observation that the cartilage defect had been repaired more effectively in the CSMP@BBR@ATDC5 group than in the other groups. Finally, the expressions of chondrogenic-related factors and SIRT1/BMP4 signaling pathway were upregulates in CSMP@BBR@ATDC5 than in other groups. In vitro, BBR protects inflammatory ATDC5 cells and maintains the expression of chondrogenic-related factors. Subsequently, we successfully use CSMP@BBR@ATDC 5 to repair knee cartilage defects in mice.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Osteoarthritis Treatment and Mechanisms · Silk-based biomaterials and applications · RNA Interference and Gene Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Orthopaedics and Traumatology, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100000, China

National Center of Orthopaedics, Beijing 100000, China

Department of Orthopaedics, Beijing Jishuitan Hospital, Capital Medical University, Beijing 100000, China

Department of Orthopaedics Surgery, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China

Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen 518036, China

Department of Sports Medicine and Rehabilitation, Peking University Shenzhen Hospital, Shenzhen 518036, China

Department of Orthopaedics, Shenzhen Second People’s Hospital, Shenzhen 518035, China

Department of Radiology, Peking University Shenzhen Hospital, Shenzhen 518036, China

Department of Spine Surgery, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen 518035, China

Funding

Peking University · Basic and Applied Basic Research Foundation of Guangdong Province · Sanming Project of Medicine in Shenzen Municipality · Shenzhen Research and Development Program · National Natural Science Foundation of China · Shenzhen University · Shenzhen High level Hospital Construction Fund · Synthetic Biology Research Center of Shenzhen University · Shenzhen Key Medical Discipline Construction Fund

References (47)

Show source-provided references
1. 10.3390/ijms22241332910.3390/ijms222413329 ↗
2. 10.1038/s41584-019-0255-110.1038/s41584-019-0255-1 ↗
3. 10.1016/j.bioactmat.2021.05.01110.1016/j.bioactmat.2021.05.011 ↗
4. 10.1021/acs.biomac.9b0004310.1021/acs.biomac.9b00043 ↗
5. 10.1021/acsbiomaterials.9b0193910.1021/acsbiomaterials.9b01939 ↗
6. 10.26599/NBE.2023.929003910.26599/NBE.2023.9290039 ↗
7. 10.2174/1574888X1366618012215190910.2174/1574888X13666180122151909 ↗
8. 10.1089/ten.teb.2020.037310.1089/ten.teb.2020.0373 ↗
9. 10.26599/FMH.2024.942000310.26599/FMH.2024.9420003 ↗
10. 10.1016/j.phymed.2019.15289210.1016/j.phymed.2019.152892 ↗
11. 10.1016/j.phrs.2020.10469010.1016/j.phrs.2020.104690 ↗
12. 10.1002/ptr.335910.1002/ptr.3359 ↗
13. 10.1016/j.ejphar.2016.07.02710.1016/j.ejphar.2016.07.027 ↗
14. 10.1002/ddr.2154110.1002/ddr.21541 ↗
15. 10.1089/ten.tea.2020.032310.1089/ten.tea.2020.0323 ↗
16. 10.1631/jzus.B150003610.1631/jzus.B1500036 ↗
17. 10.1016/j.ijbiomac.2017.06.12410.1016/j.ijbiomac.2017.06.124 ↗
18. 10.1021/acsami.2c1195610.1021/acsami.2c11956 ↗
19. 10.1039/D0BM01852J10.1039/D0BM01852J ↗
20. 10.3390/ma1002019110.3390/ma10020191 ↗
21. 10.22203/eCM.v033a0510.22203/eCM.v033a05 ↗
22. 10.1136/annrheumdis-2020-21707210.1136/annrheumdis-2020-217072 ↗
23. 10.1186/s13075-022-02983-810.1186/s13075-022-02983-8 ↗
24. 10.1007/s11010-020-04047-410.1007/s11010-020-04047-4 ↗
25. 10.18632/aging.10316110.18632/aging.103161 ↗
26. 10.1002/jev2.1208810.1002/jev2.12088 ↗
27. 10.1021/acsami.1c2126010.1021/acsami.1c21260 ↗
28. 10.1021/acs.biomac.8b0121110.1021/acs.biomac.8b01211 ↗
29. 10.3389/fbioe.2021.60770910.3389/fbioe.2021.607709 ↗
30. 10.1016/j.actbio.2017.01.03610.1016/j.actbio.2017.01.036 ↗
31. 10.1016/j.carbpol.2021.11863110.1016/j.carbpol.2021.118631 ↗
32. 10.1021/acsbiomaterials.8b0025310.1021/acsbiomaterials.8b00253 ↗
33. 10.1016/j.jep.2021.11424910.1016/j.jep.2021.114249 ↗
34. 10.1111/jcmm.1218610.1111/jcmm.12186 ↗
35. 10.1002/term.279110.1002/term.2791 ↗
36. 10.1002/stem.189010.1002/stem.1890 ↗
37. 10.1016/j.carbpol.2018.11.05010.1016/j.carbpol.2018.11.050 ↗
38. 10.1016/j.msec.2018.05.03410.1016/j.msec.2018.05.034 ↗
39. 10.1016/j.mtbio.2022.10048510.1016/j.mtbio.2022.100485 ↗
40. 10.1042/BSR2019018910.1042/BSR20190189 ↗
41. 10.1016/j.joca.2018.03.00710.1016/j.joca.2018.03.007 ↗
42. 10.1016/j.msec.2019.11028610.1016/j.msec.2019.110286 ↗
43. 10.1111/iwj.1369910.1111/iwj.13699 ↗
44. 10.1111/acel.1245610.1111/acel.12456 ↗
45. 10.2147/IJN.S18185510.2147/IJN.S181855 ↗
46. 10.1177/2041731422111461610.1177/20417314221114616 ↗
47. 10.1016/j.actbio.2018.01.03910.1016/j.actbio.2018.01.039 ↗