journal article·2024-06-10

A Pump-Free Strategy for the Controllable Generation of Alginate Microgels as Cellular Microcarriers

Xinyuan Qin, Zhongqiao Gan, Haitao Liu, Tingting Tao, Jia He, Xianliang Li, Dong Shang, Xiang Li, Fuwei Xie, Jianhua Qin

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Abstract

Microgels are advanced scaffolds for tissue engineering due to their proper biodegradability, good biocompatibility, and high specific surface area for effective oxygen and nutrient transfer. However, most of the current monodispersed microgel fabrication systems rely heavily on various precision pumps, which highly increase the cost and complexity of their downstream application. In this work, we developed a simple and facile system for the controllable generation of uniform alginate microgels by integrating a gas-shearing strategy into a glass microfluidic device. Importantly, the cell-laden microgels can be rapidly prepared in a pump-free manner under an all-aqueous environment. The three-dimensional cultured green fluorescent protein-human A549 cells in alginate microgels exhibited enhanced stemness and drug resistance compared to those under two-dimensional conditions. The pancreatic cancer organoids in alginate microgels exhibited some of the key features of pancreatic cancer. The proposed microgels showed decent monodispersity, biocompatibility, and versatility, providing great opportunities in various biomedical applications such as microcarrier fabricating, organoid engineering, and high-throughput drug screening.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: 3D Printing in Biomedical Research · Pancreatic function and diabetes · Innovative Microfluidic and Catalytic Techniques Innovation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

University of Chinese Academy of Science, Beijing 100049, China

Department of HBP Surgery, Beijing Chao Yang Hospital, the Capital Medical University, Beijing 100020, China

Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, No.222 Zhongshan Road, Dalian 116011, China

Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, No.222 Zhongshan Road, Dalian 116011, China

Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou 450001, China

Beijing Institute for Stem Cell and Regeneration, CAS, Beijing 100101, China

University of Science and Technology of China, Hefei 230026, China

Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou 215123, China

Funding

Dalian Medical University · National Key Research and Development Program of China · Dalian Institute of Chemical Physics, Chinese Academy of Sciences · China National Tobacco Corporation · National Natural Science Foundation of China

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