journal article·2022-08-08

Temporal Control over Macrophage Phenotype and the Host Response via Magnetically Actuated Scaffolds

Lindsay A. Steele, Kara L. Spiller, Smadar Cohen, Slava Rom, Boris Polyak

Publisher page

Abstract

Cyclic strain generated at the cell–material interface is critical for the engraftment of biomaterials. Mechanosensitive immune cells, macrophages regulate the host–material interaction immediately after implantation by priming the environment and remodeling ongoing regenerative processes. This study investigated the ability of mechanically active scaffolds to modulate macrophage function in vitro and in vivo. Remotely actuated magnetic scaffolds enhance the phenotype of murine classically activated (M1) macrophages, as shown by the increased expression of the M1 cell–surface marker CD86 and increased secretion of multiple M1 cytokines. When scaffolds were implanted subcutaneously into mice and treated with magnetic stimulation for 3 days beginning at either day 0 or day 5 post-implantation, the cellular infiltrate was enriched for host macrophages. Macrophage expression of the M1 marker CD86 was increased, with downstream effects on vascularization and the foreign body response. Such effects were not observed when the magnetic treatment was applied at later time points after implantation (days 12–15). These results advance our understanding of how remotely controlled mechanical cues, namely, cyclic strain, impact macrophage function and demonstrate the feasibility of using mechanically active nanomaterials to modulate the host response in vivo.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Cellular Mechanics and Interactions · Tissue Engineering and Regenerative Medicine · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Surgery, College of Medicine, Drexel University, 245 N. 15th Street, Philadelphia 19102, Pennsylvania, United States

School of Biomedical Engineering, Science and Health Systems, Drexel University, 3141 Chestnut Street, Bossone 712, Philadelphia 19104, Pennsylvania, United States

The Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel

Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel

Regenerative Medicine and Stem Cell (RMSC) Research Center, Ben-Gurion University of the Negev, Beer Sheva Blvd. 1, Bldg. 42, Room 328, Beer-Sheva 84105, Israel

Department of Pathology and Laboratory Medicine, Temple University, Philadelphia 19140, Pennsylvania, United States

Center for Substance Abuse Research, Temple University, 3500 N. Broad Street, Medical Education and Research Building, Room 842, Philadelphia 19140, Pennsylvania, United States

Funding

Israel Science Foundation · National Heart, Lung, and Blood Institute · College of Medicine, Drexel University · Louis and Bessie Stein Family Foundation · W.W. Smith Charitable Trust

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